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pJBL705

Plasmid

#140375

Purpose

Expresses TetR-regulated 3WJdB RNA aptamer driven by T7 promoter with the tetO sequence 5BP dowsntream from the promoter

Depositor

Julius Lucks

Article

Jung et al Nat Biotechnol. 2020 Jul 6. pii: 10.1038

Insert

T7-5BP-tetO-3WJdB-T

Use

Synthetic Biology

Available Since

July 8, 2020

Availability

Academic Institutions and Nonprofits only

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pSR-siKlf5

Plasmid

#41740

Depositor

Jonathan Katz

Article

Yang et al FASEB J. 2007 Feb;21(2):543-50. Epub 200

Insert

Klf5 (Klf5 Mouse)

Use

RNAi

Available Since

Jan. 18, 2013

Availability

Academic Institutions and Nonprofits only
pGEX6P1-merlin 1-339

Plasmid

#168128

Purpose

Bacterial expression of human Nf2/merlin 1-339

Depositor

Tina Izard

Article

Primi et al Matrix Biol Plus. 2021 Jul 1;12:100074.

Insert

Merlin (NF2 Human)

Tags

Glutathione S-transferase (GST)

Expression

Bacterial

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pDESTFLAGHApuro3-Ago2K533AK570A

Plasmid

#14104

Depositor

Thomas Tuschl

Article

Ma et al Nature. 2005 Mar 31. 434(7033):666-70.

Insert

Ago2 (AGO2 Human)

Tags

FLAG/HA

Expression

Mammalian

Mutation

changed Lys533 to Ala, Lys570 to Alanine

Available Since

Feb. 22, 2007

Availability

Academic Institutions and Nonprofits only
pcDNA-h-mmPtch1-B

Plasmid

#120888

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

Ptch1 (Ptch1 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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EK0545 SFFV-mCherry-SGc(GAPDH.s1)cSG-fs(EGFP)-(3xMS2) (FLP-IN)

Plasmid

#191177

Purpose

Mammalian expression of EK0493 with frameshift mutation (EGFP is frameshifted).

Depositor

Xiaojing Gao

Article

Kaseniit et al Nat Biotechnol. 2022 Oct 5. pii: 10.1038

Insert

mCherry:GAPDH.s1:EGFP (frameshifted)

Expression

Mammalian

Mutation

EGFP is frameshifted

Promoter

SFFV

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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YFP Gamma 4

Plasmid

#36104

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 4 (Gng4 Mouse)

Tags

YFP

Promoter

CMV

Available Since

May 3, 2012

Availability

Academic Institutions and Nonprofits only
pRS316-RGR-GFP-mm

Plasmid

#51059

Purpose

Same as pRS316-RGR-GFP except for the HH ribozyme region which has a 13 bp deletion at its 5' end, and for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pRS316-RGR-GFP-mHDV

Plasmid

#51058

Purpose

Same as pRS316-RGR-GFP except for the HDV ribozyme region which has a 15 bp deletion at its 3' end.

Depositor

Yunde Zhao

Article

Gao et al J Integr Plant Biol. 2013 Dec 30. doi: 1

Insert

RGR-GFP

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

Yeast ADH1 promoter

Available Since

Feb. 11, 2014

Availability

Academic Institutions and Nonprofits only

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pBS-TRE-cofilin1(S3A)-P2A-mTurquoise2

Plasmid

#163610

Purpose

Expresses constitutively active cofilin and mTurquoise2 under the TRE promoter in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

cofilin1 (Cfl1 Mouse)

Tags

mTurquoise2

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX2T merlin 340-590

Plasmid

#11633

Depositor

Vijaya Ramesh

Article

Gonzalez-Agosti et al J Biol Chem. 1999 Nov 26. 274(48):34438-

Insert

merlin (NF2 Human)

Tags

GST

Expression

Bacterial

Mutation

Carboxy-terminal half of merlin isoform 2.

Available Since

May 3, 2006

Availability

Academic Institutions and Nonprofits only
MolBC

Plasmid

#92210

Purpose

Expression of H. influenzae MolBC (ABC Transporter) in E. coli

Depositor

Douglas Rees

Article

Pinkett et al Science. 2007 Jan 19;315(5810):373-7. Ep

Inserts

MolC (HI1471 )
MolB (HI1470 )

Tags

8x Histidine Tag and Enterokinase Cleavage Site b…

Expression

Bacterial

Promoter

T7 Promoter

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA-h-mmPtch1-B-HA

Plasmid

#120902

Purpose

Expresses Ptch1-B for function assays

Depositor

Philip Beachy

Article

Zhang et al Cell. 2018 Nov 15;175(5):1352-1364.e14.

Insert

ptch1 (Ptch1 Mouse)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 620-643, truncated at 1291

Promoter

CMV

Available Since

Jan. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pPB-tetpA-iRPT

Plasmid

#140763

Purpose

Expresses iRFP713, PuroR and rtTAM2 in mammalian cells, with a TRE cassette

Depositor

Hideyuki Okano

Article

Yoshimatsu et al Neurosci Res. 2019 Oct 3. pii: S0168-010

Inserts

iRFP713
PuroR
rtTAM2

Expression

Mammalian

Available Since

Sept. 25, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-His:FRB:nls:VPR

Plasmid

#135987

Purpose

Expresses the FRB rapamycin-inducible domain fused to the VPR transcriptional activation domain in mammalian cells

Depositor

Roman Jerala

Article

Lebar et al Nat Chem Biol. 2020 Jan 6. pii: 10.1038/

Insert

FRB domain fused to the VPR activation domain

Tags

His

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pWay21-MKP-1CH2AB

Plasmid

#13475

Depositor

Anton Bennett

Article

Wu et al Mol Cell Biol. 2005 Jun . 25(11):4792-80

Insert

MKP-1CH2AB (Dusp1 Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Contains aa 1-136

Available Since

Dec. 8, 2006

Availability

Academic Institutions and Nonprofits only
pENN.AAV.CB7.CI.PM20D2-flag.WPRE.rBG

Plasmid

#132684

Purpose

AAV plasmid encoding mouse PM20D2 with C-terminal flag tag

Depositor

Jonathan Long

Article

Kim et al Cell Chem Biol. 2019 Nov 21;26(11):1623-

Insert

Peptidase M20 domain containing 2 (Pm20d2 Mouse)

Use

AAV

Tags

Flag

Promoter

Chicken b-actin

Available Since

Nov. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pAPcrRNA_Eco

Plasmid

#106276

Purpose

E.coli CRISPR Type I-E crRNA cloning vector for mammalian expression driven by U6 promoter

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pEcoCascade-IL1RNcrRNA

Plasmid

#126481

Purpose

Encodes E.coli CRISPR Type I-E crRNA targeting IL1RN

Depositor

Charles Gersbach

Article

Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

Insert

Eco IL1RN cr26

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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CBP-Bromo-HAT-SP65

Plasmid

#21090

Depositor

Corey Largman

Article

Shen et al Mol Cell Biol. 2001 Nov;21(21):7509-22

Insert

CBP (Crebbp Mouse)

Use

In vitro tnt

Tags

Flag

Mutation

Bromo+HAT domains aa 1107-1621

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only