We narrowed to 17,639 results for: URE
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Plasmid#126644PurposeCloning vector FnoCas12a-DR-GC-swap-crRNA for expression in mammalian cellsDepositorInsertFnoCas12a Full Length Direct Repeat GC Swap crRNA
UseCRISPRExpressionMammalianPromoterU6 PromoterAvailable SinceJune 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAN1779
Plasmid#127165PurposeVector for integrating the process (pGAL1 expression of Z3PM and mKate2 both fused to psd) and the circuit output (pZ3-Venus-cODC)DepositorInsertpGAL1-Z3PM-psd-tADH1-pGAL1-mKate2-psd-tPGK1-pZ3-Venus-cODC-tTDH1
UseSynthetic BiologyAvailable SinceAug. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SHANK1-1/1
Plasmid#91517PurposeProtein expression and purification of human SH3 domain construct SHANK1-1/1DepositorInsertSHANK1-1/1 (SHANK1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_NCK2-3/3
Plasmid#91256PurposeProtein expression and purification of human SH3 domain construct NCK2-3/3DepositorInsertNCK2-3/3 (NCK2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
RBMS3_2xRRM_pGEX
Plasmid#135142PurposeRecombinant expression of RBMS3 with dual-affinity tagDepositorAvailable SinceJuly 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
pSBL_DK198
Plasmid#156425PurposeInsect cell expression of 3a delta N(aa 42-275)-sfGFP, needs to be introduced into baculoviral bacmidDepositorInsertSARS-CoV-2 3a protein (aa 42-275)
TagssfGFP-HisExpressionInsectMutationaa 42-275PromoterPolyhedrinAvailable SinceAug. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
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RBM24_RRM_pGEX
Plasmid#135148PurposeRecombinant expression of RBM24 with dual-affinity tagDepositorAvailable SinceJune 3, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAGW-dpse-GAL4-DBD
Plasmid#125153PurposeGateway-compatible vector to express a fusion protein containing the GAL4-DBD (N-terminally) followed by the protein of interest under the control of a D.pseudoobscura enhancer and CPDepositorInsertD. pseudoobscura peak #102 enhancer and D. pseudoobscura CG13116 CP
UseGateway fly expression vectorExpressionInsectAvailable SinceAug. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
Bambi NGFR
Plasmid#44628DepositorAvailable SinceApril 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pmCellFree _KA2_ZIKV NS5
Plasmid#145368PurposeCell-free/mammalian expression vector for Zika virus NS5 protein with N-term mCherryDepositorInsertZIKV NS5
Tags8xHis and mCherryExpressionBacterialPromoterT7- CMV/CAGAvailable SinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 CTNNB1
Plasmid#67119PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
SLIRP.HMG6.wt.fl
Plasmid#49821Purposeexpression vector for MBP-SLIRP fusionDepositorInsertSLIRP SRA stem-loop interacting RNA binding protein (SLIRP Human)
TagsE. coli Maltose Binding ProteinExpressionBacterialMutationwildtype codon-optimized for E. coli expressionPromoterT7Available SinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
CaM-RhoA(DP)-Venus
Plasmid#31994DepositorInsertCaM-RhoA(DP) (RHOA Human)
TagsYFPExpressionBacterial and MammalianMutationQ63L mutation in RhoA, E7S and D65Y in CaMAvailable SinceJuly 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter dme-mir-125
Plasmid#46667DepositorInsertdme-mir-125
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJune 26, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PTK6-1/1
Plasmid#91441PurposeProtein expression and purification of human SH3 domain construct PTK6-1/1DepositorInsertPTK6-1/1 (PTK6 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
UKNP1.19.1
Plasmid#98192PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
ExpressionMammalianAvailable SinceAug. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS M_tTA-BFP
Plasmid#58874PurposeMESA target chain with mCherry ectodomain, no linker domain, M cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (M in P1')
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceSept. 26, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF1-1/4
Plasmid#91544PurposeProtein expression and purification of human SH3 domain construct SH3RF1-1/4DepositorInsertSH3RF1-1/4 (SH3RF1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF1-2/4
Plasmid#91457PurposeProtein expression and purification of human SH3 domain construct SH3RF1-2/4DepositorInsertSH3RF1-2/4 (SH3RF1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF1-4/4
Plasmid#91374PurposeProtein expression and purification of human SH3 domain construct SH3RF1-4/4DepositorInsertSH3RF1-4/4 (SH3RF1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF3-2/2
Plasmid#91356PurposeProtein expression and purification of human SH3 domain construct SH3RF3-2/2DepositorInsertSH3RF3-2/2 (SH3RF3 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF1-3/4
Plasmid#91366PurposeProtein expression and purification of human SH3 domain construct SH3RF1-3/4DepositorInsertSH3RF1-3/4 (SH3RF1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3RF3-1/2
Plasmid#91280PurposeProtein expression and purification of human SH3 domain construct SH3RF3-1/2DepositorInsertSH3RF3-1/2 (SH3RF3 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFLAG-4EHP(K121/130/134/222R)
Plasmid#17353DepositorAvailable SinceFeb. 19, 2008AvailabilityAcademic Institutions and Nonprofits only -
p3E-Dnm2b-DN
Plasmid#109574PurposeMultisite gateway vector for 3' tagging with dominant negative Dynamin2bDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
p3E-EHD1b-dEH
Plasmid#109567PurposeMultisite gateway vector for 3' tagging with EHD1b-delta-EHDepositorAvailable SinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT1100
Plasmid#8618DepositorInsertribonuclease Sa3, barstar
TagsphoA signal sequence to RNase Sa3ExpressionBacterialMutationIn addition to the substitution of the barnase ge…Available SinceJan. 17, 2007AvailabilityAcademic Institutions and Nonprofits only -
pKP4010
Plasmid#113336PurposeBeta-estradiol inducible 3X-FLAG Ty3 Integrase; Z4 promoter-3XFLAG Ty3 IN-CYC1 terminator; artificial transcription factor under ACT1 promoterDepositorInsertsArtificial Transcription Factor (McIsaac et al, 2013)
Ty3 Integrase
Tags3X-FLAG/8X-Gly linkerExpressionYeastAvailable SinceAug. 16, 2018AvailabilityAcademic Institutions and Nonprofits only -
mCherry_LD 0_CS A_tTA-BFP
Plasmid#58873PurposeMESA target chain with mCherry ectodomain, no linker domain, A cleavage sequence, and tTA-BFP fusionDepositorInsertMESA target chain with mCherry ectodomain, cd28 transmembrane domain, mutated TEV cleavage sequence (A in P1')
UseAAVTagsEBFPExpressionMammalianPromoterCMVAvailable SinceNov. 10, 2014AvailabilityAcademic Institutions and Nonprofits only -
HM3C NaVSp1_p pET24b
Plasmid#127852PurposeBacterial expression vector for NaVSp1 bacterial sodium channel residues 122-258. It expresses HIStag-MBP-3C-channel protein.DepositorInsertNaVSp1
Tags3C Cleavage site, His Tag, and MBPExpressionBacterialAvailable SinceMay 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
-962 human cyclin D1 promoter TCF (1)-(3) sites mutant pGL3Basic
Plasmid#32735DepositorInsertCCND1 Promoter (CCND1 Human)
UseLuciferaseMutation-75 TCF(1) and -68 TCF(2) CTTTGATCTTTGCT deletion…Promoter-962 CCND1Available SinceJan. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
Psicheck2 Sox4 WT 3'UTR oligo
Plasmid#26990DepositorInsertSox4 wt UTR oligo (SOX4 Human)
UseLuciferaseTagsRenilla LuciferaseExpressionMammalianMutationbp 449-509 of SOX4 3' UTRAvailable SinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SH3BP4-1/1
Plasmid#91532PurposeProtein expression and purification of human SH3 domain construct SH3BP4-1/1DepositorInsertSH3BP4-1/1 (SH3BP4 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SORBS2-1/3
Plasmid#91387PurposeProtein expression and purification of human SH3 domain construct SORBS2-1/3DepositorInsertSORBS2-1/3 (SORBS2 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_VAV1-1/2
Plasmid#91355PurposeProtein expression and purification of human SH3 domain construct VAV1-1/2DepositorInsertVAV1-1/2 (VAV1 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOGG113
Plasmid#133124PurposepL0M-PU-NoP, promotorless region used as negative controlDepositorInsertNoP promotorless region from plasmid pIJJ11268
ExpressionBacterialMutationDomesticated for Golden Gate cloningAvailable SinceDec. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_STAM-1/1
Plasmid#91428PurposeProtein expression and purification of human SH3 domain construct STAM-1/1DepositorInsertSTAM-1/1 (STAM Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PACSIN3-1/1
Plasmid#91335PurposeProtein expression and purification of human SH3 domain construct PACSIN3-1/1DepositorInsertPACSIN3-1/1 (PACSIN3 Human)
ExpressionBacterialAvailable SinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
M26V DJ-1 pET15b
Plasmid#60688PurposeRecombinant protein expression of Park7DepositorAvailable SinceOct. 24, 2014AvailabilityAcademic Institutions and Nonprofits only -
pMath3-luc
Plasmid#51312PurposeLuciferase reporter driven by the Math3 promoterDepositorAvailable SinceMarch 7, 2014AvailabilityAcademic Institutions and Nonprofits only -
pDESTFLAGHApuro3-Ago2Q545AK570A
Plasmid#14105DepositorInsertAgo2 (AGO2 Human)
TagsFLAG/HAExpressionMammalianMutationchanged Gln545 to Ala and Lys570 to AlaAvailable SinceFeb. 22, 2007AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 mir-1-1 reporter mir129-2-wt
Plasmid#46730DepositorInsertmir129-2-wt (MIR129-2 Synthetic, Human)
UseRNAiTagsV5 and hsa-mir-1-1ExpressionMammalianPromoterCMVAvailable SinceJune 29, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 GTF2H4
Plasmid#67094PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pENTR-R4R3-stop
Plasmid#113745PurposeContains three stop codons in every frame to ensure a premature stop codon when introduced into the genome via homology-directed repair. Gateway multisite entry clone.DepositorInsertstop cassette
UseCRISPR; Gateway entry vectorMutationAdded 3 stop codons to the original sequence; see…Available SinceSept. 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCellFree_G03 MAPK13
Plasmid#67077PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pIS47 C20orf139 3'UTR mut
Plasmid#14490DepositorInsertC20orf139 3'UTR mutant (SRXN1 Human)
UseLuciferaseTagsluciferaseExpressionMammalianMutationCATTCC’s were changed to AAGTAC'sAvailable SinceMarch 7, 2007AvailabilityAcademic Institutions and Nonprofits only -
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pCellFree_G03 ATF3
Plasmid#67057PurposeStandard for cell-free expression benchmarking.DepositorAvailable SinceNov. 6, 2015AvailabilityAcademic Institutions and Nonprofits only