We narrowed to 5,491 results for: SPI

1. pPRC6-ProD

   Plasmid

   #198431

   Purpose

   mkate2 fluorescent reporter gene under control of promoter ProD. Used as a control plasmid in reporter gene assays.

   Depositor

   Eveline Peeters

   Article

   Bernauw et al Microbiologyopen. 2023 Jun;12(3):e1356.

   Insert

   mKate2
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   ProD and no promoter

   Available since

   March 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pPB EF1a CCR6-eLOV-TEVcs-Gal4

   Plasmid

   #173119

   Purpose

   Expresses CCR6 eLOV receiver construct in DIPG

   Depositor

   Alice Ting

   Article

   Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

   Insert

   CCR6-eLOV-TEVcs-Gal4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPB EF1a CCR6-hLOV-TEVcs-Gal4

   Plasmid

   #173120

   Purpose

   Expresses CCR6 hLOV receiver construct in DIPG

   Depositor

   Alice Ting

   Article

   Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

   Insert

   CCR6-hLOV-TEVcs-Gal4
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pAAV CMV CCR6-eLOV-TEVcs-Gal4

   Plasmid

   #173113

   Purpose

   Expresses CCR6 receiver construct in mammalian cells

   Depositor

   Alice Ting

   Article

   Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

   Insert

   CCR6-eLOV-TEVcs-tTA
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Dec. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGPN

   Plasmid

   #187482

   Purpose

   Expression vector - GFP

   Depositor

   Lewis Cantley

   Article

   Kastenhuber et al Elife. 2022 Mar 23;11. pii: 77444. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   EFS

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pET28_redesigned_Caspase-4_ANR261ENP_D315H_M318K_T352K_enzymatic domain

   Plasmid

   #183387

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   redesigned Caspase-4 (AA94-377) (CASP4 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   ANR261ENP, D315H, M318K, T352K

   Promoter

   T7

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pMSCV-IRES-GFP-mouse-Caspase-1-H340D

   Plasmid

   #183362

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1 H340D (Casp1 Mouse)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   H340D

   Promoter

   MESV

   Available since

   July 18, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pMSCV-IRES-GFP-dog-Caspase-1/4a-triple mutant-H342D_K345M_K379T

   Plasmid

   #183364

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1/4a (CASP4 Canis lupus familiaris)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   H342D_K345M_K379T

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pMSCV-IRES-GFP-dog-Caspase-1/4b

   Plasmid

   #183365

   Purpose

   Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   Caspase-1/4b (CASP4 Canis lupus familiaris)
   

   Use

   Retroviral

   Tags

   Myc

   Expression

   Mutation

   Promoter

   MESV

   Available since

   May 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMSCV-IRES-GFP-dog-Caspase-1/4a

    Plasmid

    #183363

    Purpose

    Stable expression of mammalian inflammatory caspase gene in mammalian cells by retroviral transduction

    Depositor

    Jonathan Kagan

    Article

    Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

    Insert

    Caspase-1/4a (CASP4 Canis lupus familiaris)
    

    Use

    Retroviral

    Tags

    Myc

    Expression

    Mutation

    Promoter

    MESV

    Available since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TLCV2-sgHPDL #3

    Plasmid

    #174165

    Purpose

    knock out HPDL in human cell lines

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    sgRNA against HPDL
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. TLCV2-sgHPDL #9

    Plasmid

    #174166

    Purpose

    knock out HPDL in human cell lines

    Depositor

    Michael Pacold

    Article

    Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

    Insert

    sgRNA against HPDL
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGBW-m4136457

    Plasmid

    #149537

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 M (membrane)

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 M (membrane) (M Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    6xHIS;Linker_GGSG;GST;Linker_GGSG;Cleavable TEV

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pGBW-m4095418

    Plasmid

    #149525

    Purpose

    Bacterial expression plasmid for SARS-CoV-2 nsp3

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp3 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;6xHIS

    Expression

    Bacterial

    Mutation

    d1-1088 d1203-1945

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    May 14, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

15. pGBW-m4046784

    Plasmid

    #145704

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

16. pGBW-m4046911

    Plasmid

    #145695

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 helicase

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 helicase (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

17. pGBW-m4046838

    Plasmid

    #145696

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 endoRNAse

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 endoRNAse (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;HA

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

18. pGBW-m4046840

    Plasmid

    #145700

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp10

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp10 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;StrepII

    Expression

    Bacterial

    Mutation

    Escherichia coli recode 1

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

19. pGBW-m4046787

    Plasmid

    #145690

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 nsp9

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 nsp9 (ORF1ab Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

20. pGBW-m4046767

    Plasmid

    #145692

    Purpose

    Bacterial Expression plasmid for SARS-CoV-2 ORF6 protein

    Depositor

    Benjie Chen, Ginkgo Bioworks

    Insert

    SARS-CoV-2 ORF6 protein (ORF6 Escherichia coli str. K-12 substr. MG1655; Severe acute respiratory syndrome coronavirus 2)
    

    Use

    Tags

    Cleavable TEV;Myc

    Expression

    Bacterial

    Mutation

    wt

    Promoter

    Promoter | pT7 ; Operator | lacO ; RBS | T7_rbs ;…

    Available since

    April 29, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge