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MC177VSG221RNAi

Plasmid

#59730

Purpose

For use in trypanosome cell lines already expressing T7 polymerase and Tet-repressor. Tetracycline induction results in the production of dsRNA targeting VSG221.

Depositor

Gloria Rudenko

Article

Sheader et al Proc Natl Acad Sci U S A. 2005 Jun 14;10

Insert

VSG221 RNAi fragment

Use

RNAi

Promoter

opposing T7 promoters

Available Since

Aug. 6, 2015

Availability

Academic Institutions and Nonprofits only

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xCas9(3.6)-BE4

Plasmid

#108386

Purpose

Mammalian expression vector for xCas9(3.6)-BE4

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.6)-BE4

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-evoCjCas9-ABE8e_gRNA

Plasmid

#202561

Purpose

hSyn-evoCjCas9-ABE8e and hU6-BsmBI-gRNA in AAV2 backbone (ITRs)

Depositor

Gerald Schwank

Article

Schmidheini et al Nat Chem Biol. 2023 Sep 21. doi: 10.1038

Insert

evoCjCas9-ABE8e

Use

AAV

Available Since

Sept. 21, 2023

Availability

Academic Institutions and Nonprofits only

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ECFP-ER-0

Plasmid

#55342

Purpose

Localization: Endoplasmic Reticulum, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

ER (CALR Human)

Tags

ECFP

Expression

Mammalian

Mutation

ER Signal Peptide

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits
xCas9(3.6)-BE3

Plasmid

#108385

Purpose

Mammalian expression vector for xCas9(3.6)-BE3

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.6)-BE3

Tags

NLS

Expression

Mammalian

Mutation

E108G, S217A, A262T, S409I, E480K, E543D, M694I, …

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Vimentin-C-18

Plasmid

#55157

Purpose

Localization: Intermediate Filaments, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Article

Rizzo et al Cold Spring Harb Protoc. 2009 Dec;2009(1

Insert

Vimentin (VIM Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only
pEY41

Plasmid

#191045

Purpose

dat-1 fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

dat-1 (dat-1 Nematode)

Expression

Worm

Mutation

None

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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PRS_8-jRGECO1a

Plasmid

#210399

Purpose

AAV-vector for fluorescent calcium measurements in the red spectrum from noradrenergic neurons

Depositor

Simon Wiegert

Article

Wissing et al PLoS Biol. 2025 Jul 7;23(7):e3003228. do

Insert

jRGeco1a

Use

AAV

Expression

Mammalian

Promoter

PRSx8

Available Since

Sept. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Plasmid

#159859

Purpose

Three-fragment Gateway compatible flourophore for expression in C. elegans

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

[1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 18, 2020

Availability

Academic Institutions and Nonprofits only

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MS2_adRNA-MCP_ADAR2 DD (E488Q)_NLS

Plasmid

#124705

Purpose

Expresses MS2_adRNA targeting the RAB7A transcript along with the MCP_ADAR2 DD(E488Q)_NLS

Depositor

Prashant Mali

Article

Katrekar et al Nat Methods. 2019 Mar;16(3):239-242. doi

Insert

MCP_ADAR2 DD (E488Q)_NLS

Use

AAV

Tags

NLS

Expression

Mammalian

Mutation

E488Q hyperactive mutant

Promoter

CMV

Available Since

May 14, 2019

Availability

Academic Institutions and Nonprofits only

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pDAS12342_PEAR-GFP_2in1

Plasmid

#177184

Purpose

PEAR-GFP plasmid with a pegRNA that targets its own plasmid

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Inserts

EGFP split with an intron between amino acids 95-96
pegRNA targeting its own plasmid

Use

CRISPR

Expression

Mammalian

Mutation

disrupted 5' splice site

Promoter

CMV and U6

Available Since

Feb. 25, 2022

Availability

Academic Institutions and Nonprofits only

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pMDJ17 - pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Plasmid

#159803

Purpose

Expression construct with ubiquitous expression to test fluorescence level on microscope

Depositor

Christian Froekjaer-Jensen

Article

Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

Insert

pEXP[Peft-3 | dendra2 (NLS) | tbb-2 UTR]

Expression

Worm

Mutation

Not applicable

Available Since

Dec. 17, 2020

Availability

Academic Institutions and Nonprofits only

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mTFP1-Vinculin-23

Plasmid

#55516

Purpose

Localization: Focal Adhesions, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

Vinculin (VCL Human)

Tags

mTFP1

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
pDAS12137_sgRNA-PEAR-GFP-nick(+17)-mCherry

Plasmid

#177183

Purpose

plasmid expressing an sgRNA targeting the PEAR-GFP plasmid along with an mCherry marker

Depositor

Ervin Welker

Article

Simon et al Elife. 2022 Feb 23;11. pii: 69504. doi:

Insert

sgRNA targeting the PEAR-GFP plasmid

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Feb. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pDest-303

Plasmid

#159678

Purpose

Gateway destination vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Type

Empty backbone

Expression

Mammalian

Available Since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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pAB076i5

Plasmid

#79199

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2+3) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2+3); Ppro1

Available Since

Oct. 7, 2016

Availability

Academic Institutions and Nonprofits only

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pAB092a

Plasmid

#79217

Purpose

Directed Evolution of Protein-Protein Interactions

Depositor

David Liu

Article

Badran et al Nature. 2016 May 5;533(7601):58-63. doi:

Insert

PlacZ-opt (OR1+2) gIII, luxAB; Ppro1 434cI-SH2ABL1

Expression

Bacterial

Promoter

PlacZ-opt (OR1+2); Ppro1

Available Since

Nov. 8, 2016

Availability

Academic Institutions and Nonprofits only

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pYDE007

Plasmid

#194152

Purpose

sgRNA expression in A. baumannii for CRISPRi. Replicative plasmid, CarbR. Contains mrfp nontargeting control guide sequence.

Depositor

Edward Geisinger

Article

Bai et al J Bacteriol. 2021 May 20;203(12):e005652

Insert

sgRNA with mrfp control guide sequence

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 7, 2023

Availability

Academic Institutions and Nonprofits only

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pMXs-Sig10-Fc-IG

Plasmid

#183722

Purpose

Expresses the fusion protein containing the extracellular part of human Siglec-10 and the Fc region of human IgG1, and GFP

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

Insert

Siglec-10

Use

Retroviral

Tags

Fc of human IgG1

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFRT-PonA-BI-Gl-WT-24xMS2-PTC-18xPP7 (WP)

Plasmid

#189792

Purpose

Express wild-type and PTC-containing beta-globin genes bi-directionally under ponasteron A inducible promoter in mammalian cells

Depositor

Robert Singer

Article

Sato et al Nat Commun. 2021 Dec 10;12(1):7203. doi:

Insert

beta-globin (HBB Mouse, Human)

Expression

Mammalian

Promoter

Ponasterone A inducible

Available Since

March 9, 2023

Availability

Academic Institutions and Nonprofits only

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