We narrowed to 52,914 results for: plasmid

1. pLIX403-EBFP2-ccdB-NatMx

   Plasmid

   #158559

   Purpose

   Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

   Depositor

   Alejandro Chavez

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   EBFP2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. DFNB31-TwinStrepII

   Plasmid

   #83196

   Purpose

   Expression of tagged protein in mammalian cells

   Depositor

   Peter Barr-Gillespie

   Insert

   DFNB31 (Whrn Mouse)
   

   Use

   Tags

   Twin Strep Tag II

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. FU_tetO_dCas9-VP192-T2A-EGFP

   Plasmid

   #121173

   Purpose

   Lentiviral construct for inducible expression of dCas9-VP192 transcriptional activator and EGFP.

   Depositor

   Timo Otonkoski

   Insert

   dCas9-VP192-T2A-EGFP
   

   Use

   CRISPR and Lentiviral; Inducible by doxycycline

   Tags

   T2A-EGFP-ires-puro

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCR8/GW/TOPO PCGF2

   Plasmid

   #69791

   Purpose

   Gateway entry vector for PCGF2

   Depositor

   Gerard Cagney

   Insert

   PCGF2 (PCGF2 Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 23, 2015

   Availability

   Academic Institutions and Nonprofits only

5. pET28a_PTK2b

   Plasmid

   #224237

   Purpose

   Expression of human PTK2b Kinase in E. coli

   Depositor

   Andrew Mesecar

   Insert

   ptk2b (PTK2B Human)
   

   Use

   Tags

   6xHis and 6xHis-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promotor

   Available since

   Jan. 16, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. Optopatch2 (QuasAr2-mOrange2-P2A-CheRiff-eGFP)

   Plasmid

   #51656

   Purpose

   hsyn promoting optopatch, which includes a ChR64, GFP, Quasar2 and mOrange

   Depositor

   Adam Cohen

   Insert

   Quasar2-mOrange-Chr64-GFP
   

   Use

   Lentiviral

   Tags

   GFP and mOrange

   Expression

   Mammalian

   Mutation

   Promoter

   human synapsin

   Available since

   March 10, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. acat 1-906

   Plasmid

   #24194

   Depositor

   David Rimm

   Insert

   alpha catenin (CTNNA1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Full length

   Promoter

   Available since

   March 9, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDD400

   Plasmid

   #137024

   Purpose

   SapTrap mNG-GLO donor

   Depositor

   Daniel Dickinson, Bob Goldstein

   Insert

   C. elegans GLO-optimized mNeonGreen
   

   Use

   CRISPR; Saptrap donor for cloning repair templat…

   Tags

   C. elegans GLO-optimized mNeonGreen

   Expression

   Worm

   Mutation

   Promoter

   Available since

   March 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. YEpADE1

   Plasmid

   #22495

   Depositor

   Beth Montelone

   Insert

   ADE1 (ADE1 Budding Yeast)
   

   Use

   Yeast shuttle

   Tags

   Expression

   Mutation

   Original 4067 bp HindIII-HindIII insertion (see Y…

   Promoter

   Available since

   Jan. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBHM2642

    Plasmid

    #229526

    Purpose

    Carries OsTIR1-t2a-mNG-mIAA7_opt, used to insert TIR1 expressing construct into the C. neoformans genome.

    Depositor

    Hiten Madhani

    Insert

    OsTIR1
    

    Use

    Tags

    t2a-mNeonGreen-mIAA7_opt

    Expression

    Bacterial and Yeast

    Mutation

    F74G

    Promoter

    Available since

    March 26, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pET11a_PTK2b_Kinase

    Plasmid

    #224242

    Purpose

    Expression of human PTK2b Kinase domain in E. coli

    Depositor

    Andrew Mesecar

    Insert

    ptk2b_kinase (PTK2B Human)
    

    Use

    Tags

    6xHis-TEV

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promotor

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pDONR221-WT-long-UBASH3A-Ntag

    Plasmid

    #192098

    Purpose

    Human wild-type long-form UBASH3A in Gateway pDONR221 (for N-terminal tag)

    Depositor

    Patrick Concannon

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pDONR221-WT-long-UBASH3A-Ctag

    Plasmid

    #192097

    Purpose

    Human wild-type long-form UBASH3A in Gateway pDONR221 (for C-terminal tag)

    Depositor

    Patrick Concannon

    Insert

    UBASH3A (UBASH3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLIX403-EYFP-ccdB-Blast

    Plasmid

    #158551

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLU-CMV-PL3 Flag-humanTRAF2-WT (1108)

    Plasmid

    #66924

    Purpose

    3rd generation lentiviral Expression of TRAF2

    Depositor

    Michael Karin

    Insert

    TRAF2 (TRAF2 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. MEIS1A-pVP16

    Plasmid

    #21016

    Depositor

    Corey Largman

    Insert

    MEIS1A (Meis1 Mouse)
    

    Use

    Tags

    VP16-AD

    Expression

    Mammalian

    Mutation

    VP16 activation domain fused to N-terminal

    Promoter

    Available since

    Aug. 14, 2009

    Availability

    Academic Institutions and Nonprofits only

17. WARS_pNIC-CTB10H

    Plasmid

    #153058

    Purpose

    Expression construct for producing Avi tagged Tryptophanyl-tRNA synthetase

    Depositor

    Susanne Gräslund

    Insert

    WARS (WARS1 Human)
    

    Use

    Tags

    TEV-Avi-His10

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pHEE-R-kan5A9si

    Plasmid

    #222836

    Purpose

    CRISPR/Cas9 for multi-gene knockout in Arabidopsis (single intron-containing Cas9, RPS5A promoter, kanamycin and red fluorescent seed selection)

    Depositor

    Hidenori Takeuchi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Oct. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pT7-MS2-HA-C1-HsYTHDF3_AB

    Plasmid

    #148322

    Purpose

    Mammalian Expression of HsYTHDF3

    Depositor

    Elisa Izaurralde

    Insert

    HsYTHDF3 (YTHDF3 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p404GAL1

    Plasmid

    #17417

    Depositor

    Nicolas Buchler, Fred Cross

    Insert

    GAL1 promoter + polylinker + CYC1 terminator (GAL1 Budding Yeast)
    

    Use

    Tags

    Expression

    Bacterial and Yeast

    Mutation

    GAL1 promoter is between SacI and XbaI restrictio…

    Promoter

    Available since

    March 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. ecALDH

    Plasmid

    #67921

    Purpose

    Expresses aldehyde dehydrogenase from Escherichia coli, codon optimized for expression in E. coli

    Depositor

    Justin Siegel

    Insert

    Escherichia coli aldehyde dehydrogenase (puuC Escherichia coli)
    

    Use

    Tags

    6x-His

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. AiP909-Bxb1-AttP-PGK-gb2-Neo-PGK-Bxb1-AttP-SA-hygro2

    Plasmid

    #181778

    Purpose

    Bxb1 AttP in TIGRE landing pad vector

    Depositor

    Tanya Daigle

    Insert

    Neomycin
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. katushka-FMDV-P2A-pTRIP

    Plasmid

    #163522

    Purpose

    Used to construct lentivirus to express katushka in mammalian cells with option for a second insert

    Depositor

    Thomas Weber

    Insert

    stopless Katushka-P2A
    

    Use

    Lentiviral

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pLIX403-EBFP2-ccdB-Puro

    Plasmid

    #158552

    Purpose

    Set of empty Gateway Cloning compatible, inducible, lentiviral vectors with various mammalian selection markers and N-terminal fluorescent protein fusions

    Depositor

    Alejandro Chavez

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    EBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. LZRS-Mek2-wt (2-3)

    Plasmid

    #21201

    Depositor

    Paul Khavari

    Insert

    Mek2 (MAP2K2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 9, 2009

    Availability

    Academic Institutions and Nonprofits only

26. PP-G6PI

    Plasmid

    #28283

    Depositor

    Cheryl Arrowsmith

    Insert

    PP-G6PI (PF14_0341 Plasmodium falciparum)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 14, 2011

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

27. pLG1 library vector (pU6-sgRNA Ef1alpha-Puro-T2A-BFP)

    Plasmid

    #217305

    Purpose

    sgRNA BFP + Puromycin

    Depositor

    Luke Gilbert

    Insert

    pU6-sgRNA, Ef1alpha-Puro-T2A-BFP
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    U6, Ef1alpha

    Available since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMT ERK1

    Plasmid

    #12656

    Depositor

    John Kyriakis

    Insert

    ERK1 (Mapk3 Rat)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 21, 2006

    Availability

    Academic Institutions and Nonprofits only

29. TC9pAB

    Plasmid

    #71310

    Purpose

    used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

    Depositor

    Karl Degenhardt

    Insert

    Cas9
    

    Use

    In vitro transcription

    Tags

    NLS

    Expression

    Mutation

    Promoter

    T7

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pEBG-hPKAalpha-Flag

    Plasmid

    #185381

    Purpose

    For mammalian expression of human GST-PKAalpha-FLAG

    Depositor

    Ayaz Najafov

    Insert

    PRKACA (PRKACA Human)
    

    Use

    Tags

    FLAG and GST

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    Available since

    June 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCLXSN(GFP)-hp100 SS/AA

    Plasmid

    #174735

    Purpose

    Gene overexpression

    Depositor

    Shao-Cong Sun

    Insert

    p100 SS/AA (NFKB2 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    SS/AA

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pGAPZ-STE2-1D4

    Plasmid

    #133423

    Purpose

    Yeast STE2 coding sequence in vector for protein expression in the methanogenic yeast Pichia pastoris, with GAP promoter.

    Depositor

    Gerhard Wagner

    Insert

    STE2 (STE2 Budding Yeast)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    C59S, C252A. No alpha factor secretion signal in …

    Promoter

    Available since

    Jan. 3, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pBabepuro-IL11

    Plasmid

    #189609

    Purpose

    Retroviral expression vector for IL11

    Depositor

    Thierry Tchenio

    Insert

    IL11 (IL11 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    SV40 early promoter

    Available since

    Aug. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. Lenti-EF1alpha-VP64-dCas9-VP64-ZsG

    Plasmid

    #192667

    Purpose

    3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A ZsGreen1

    Depositor

    Jeffrey Miner

    Insert

    VP64-dCas9-VP64, ZsGreen1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    D10A and N863A in Cas9

    Promoter

    EF1a

    Available since

    Dec. 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. Cul1-EYFP

    Plasmid

    #111214

    Purpose

    fluorescent fusion protein

    Depositor

    Johannes A. Schmid

    Insert

    CUL1 (CUL1 Human)
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pGEX-5X-1 U1-C-FL

    Plasmid

    #129077

    Purpose

    generation of GST-tagged U1-C protein using bacteria

    Depositor

    Takeshi Tsubata

    Insert

    U1-C (SNRPC Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pcDNA3-Xiap-Myc K322/328R

    Plasmid

    #11834

    Depositor

    Guy Salvesen

    Insert

    XIAP K322/328R (XIAP Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    K322/328R

    Promoter

    Available since

    May 12, 2006

    Availability

    Academic Institutions and Nonprofits only

38. pAAV-tMCK-Cyto-TurboID

    Plasmid

    #149411

    Purpose

    Muscle-specific expression of cytosolic TurboID, N-term V5 tag, in AAV expression plasmid. Amp selection

    Depositor

    Jonathan Long

    Insert

    Cyto-TurboID
    

    Use

    AAV

    Tags

    V5

    Expression

    Mutation

    Promoter

    tMCK

    Available since

    Nov. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pDSP32

    Plasmid

    #199374

    Purpose

    Encodes dual cistronic nuclear localized jGCaMP7s and mScarlet-I optimized for C. elegans expression

    Depositor

    Hang Lu

    Insert

    NLS::jGCaMP7s::egl-13NLS::SL2::NLS::mScarlet-I::egl-13NLS
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    no mutations, except added subcellular localizati…

    Promoter

    Available since

    April 25, 2023

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

40. pET15b/GgVcl 884-1066-ECFP

    Plasmid

    #46182

    Depositor

    Susan Craig

    Insert

    Vcl 884-1066 (VCL Chicken)
    

    Use

    Tags

    ECFP and His

    Expression

    Bacterial

    Mutation

    aa 884-1066 (tail domain)

    Promoter

    T7

    Available since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

41. pBS-MK3

    Plasmid

    #30190

    Depositor

    Bruce Tempel

    Insert

    MK-3 (Kcna3 Mouse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 3, 2011

    Availability

    Academic Institutions and Nonprofits only

42. pUC57_beta-globin-5'UTR_Nanoluc

    Plasmid

    #219989

    Purpose

    betaglobin-5'UTR-NanoLuc mRNA synthesis

    Depositor

    Simon Alberti

    Insert

    betaglobin-5'UTR-NanoLuc (HBB Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    June 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

43. pJR255

    Plasmid

    #78549

    Purpose

    For expressing small noncoding RNAs from U6 promoter. One step cloning of oiigonucleotide pairs containing CACC and AAAA overhangs. CMV driven mCherry visible marker.

    Depositor

    John Rossi

    Type

    Empty backbone

    Use

    RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6, CMV

    Available since

    Jan. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

44. IDG_PANX2_OE_1

    Plasmid

    #161697

    Purpose

    Overexpresses PANX2

    Depositor

    Mike McManus

    Insert

    PANX2 (PANX2 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

45. pLNHA-C1-HseIF4E_K

    Plasmid

    #146732

    Purpose

    Mammalian Expression of HseIF4E

    Depositor

    Elisa Izaurralde

    Insert

    HseIF4E (EIF4E Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

46. pET14b_tev_PDZ1

    Plasmid

    #59739

    Purpose

    expresses PDZ1 domain of human ZO-1 in bacteria

    Depositor

    Alan Fanning

    Insert

    zonula occludens-1 (TJP1 Human)
    

    Use

    Tags

    HIS tag and thrombin clvg

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

47. pcDNA-p50

    Plasmid

    #107978

    Purpose

    Expresssion of NF-kB p50

    Depositor

    Georg Weber

    Insert

    NF-kB p50 (Nfkb1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Amino acids VQACI added to C-terminus

    Promoter

    CMV

    Available since

    Dec. 3, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

48. pcDNA3 mId1

    Plasmid

    #16060

    Depositor

    Robert Benezra

    Insert

    Id1 (Id1 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

49. SPHK1

    Plasmid

    #73260

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    SPHK1 (SPHK1 Human)
    

    Use

    Tags

    His-6-TEV

    Expression

    Insect

    Mutation

    Promoter

    Polyhedrin

    Available since

    April 21, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

50. pKT-PDGFRa-IRES-Katushka

    Plasmid

    #133081

    Purpose

    Expresses PDGFRa receptor and katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

    Depositor

    Maria Castro

    Insert

    PDGFRa (Pdgfra Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge