We narrowed to 13,808 results for: TIM

1. pcDNA3.1-OGT_opt

   Plasmid

   #190858

   Purpose

   For mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.

   Depositor

   Gerald Hart

   Article

   Zhu et al Cell. 2022 Dec 30:S0092-8674(22)01529-X.

   Insert

   O-GlcNAc Transferase (OGT Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Codon optimized for expression in human cells.

   Promoter

   CMV

   Available since

   Jan. 9, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. FLAG-p54

   Plasmid

   #35379

   Depositor

   Benjamin Blencowe

   Article

   Rosonina et al Mol Cell Biol. 2005 Aug;25(15):6734-46.

   Insert

   p54 (NONO Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   A464V

   Promoter

   CMV

   Available since

   March 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMIR-Dsred-IRES-His-Halo-Tev-Keap C151S

   Plasmid

   #62458

   Purpose

   expresses his-tagged C151S human Keap1 protein

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Keap1 (KEAP1 Human)
   

   Use

   Tags

   His-Halo-Tev

   Expression

   Mammalian

   Mutation

   Changed Cysteine 151 of Keap1 to serine

   Promoter

   CMV

   Available since

   March 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. CMV500 A-CREB

   Plasmid

   #33371

   Depositor

   Charles Vinson

   Article

   Ahn et al Mol Cell Biol. 1998 Feb . 18(2):967-77.

   Insert

   CREB (Creb1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Dominant Negative (see notes)

   Promoter

   CMV

   Available since

   Dec. 19, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. RGS-6xHis-BLIMP-1-pcDNA3.1-

   Plasmid

   #52518

   Purpose

   expresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Blimp1 (PRDM1 Human)
   

   Use

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA3-AKAR4-NES

   Plasmid

   #64727

   Purpose

   Cytosol-targeted, FRET-based PKA activity reporter.

   Depositor

   Jin Zhang

   Article

   Herbst et al Mol Cell Biol. 2011 Oct;31(19):4063-75.

   Insert

   AKAR4-NES
   

   Use

   Tags

   Cerulean, NES, and cpVenus[E172]

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCDNA5/FRT/TO_TOMM20_HaloTag9_T2A_EGFP

   Plasmid

   #169333

   Purpose

   Expression of HaloTag9 at the mitochondrial surface of mammalian cells

   Depositor

   Kai Johnsson

   Article

   Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

   Insert

   TOMM20-HaloTag9 (TOMM20 Human)
   

   Use

   Tags

   HaloTag9

   Expression

   Mammalian

   Mutation

   HaloTag9 = HaloTag7-Q165H-P174R

   Promoter

   Available since

   Nov. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBBR1.cas9-NT

   Plasmid

   #197871

   Purpose

   Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)

   Depositor

   Baojun Wang

   Article

   Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72

   Inserts

   trancCRNA
   Cas9
   crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. pet28a-His6-Halo-TEV-Nrf2

   Plasmid

   #62455

   Purpose

   Halo-His6 tagged Nrf2 for E.coli expression

   Depositor

   Yimon Aye

   Article

   Parvez et al J Am Chem Soc. 2014 Dec 31.

   Insert

   Nrf2 (NFE2L2 Human)
   

   Use

   Tags

   His6-Halo-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   April 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pLenti-EFS-MCS-WPRE

    Plasmid

    #163362

    Purpose

    The empty pLenti backbone with multiple cloning site that allows shuttling between pMY and pMX vectors

    Depositor

    Louis Ates, Teunis Geijtenbeek

    Article

    van der Donk et al Biol Open. 2022 Feb 15;11(2):bio059032.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. p415 TEF roGFP2‐Tsa2ΔCR

    Plasmid

    #83238

    Purpose

    The genetically encoded fluorescent H2O2 sensor roGFP2-Tsa2ΔCR cloned in the yeast p415 vector under the control of a TEF promoter. For cytosolic expression.

    Depositor

    Tobias Dick

    Article

    Morgan et al Nat Chem Biol. 2016 Jun;12(6):437-43. do

    Insert

    roGFP2-Tsa2dCr
    

    Use

    Tags

    roGFP2 fusion with Tsa2dCr

    Expression

    Yeast

    Mutation

    Promoter

    TEF

    Available since

    Sept. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLNCX2-mCherry-CHMP2A

    Plasmid

    #115330

    Purpose

    expresses mCherry-CHMP2A in mammalian cells

    Depositor

    Sanford Simon

    Article

    Johnson et al Elife. 2018 Jul 4;7. pii: 36221. doi: 10

    Insert

    CHMP2A (CHMP2A Human)
    

    Use

    Retroviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 28, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. lenti CAG-FLAG-dCas9-VPR

    Plasmid

    #114197

    Purpose

    Lentivirus compatible dCas9-VPR construct driven by the CAG promoter

    Depositor

    Jeremy Day

    Article

    Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

    Insert

    dCas9-VPR
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Sept. 10, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. mEGFP-HRas G12V

    Plasmid

    #18666

    Depositor

    Karel Svoboda

    Article

    Yasuda et al Nat Neurosci. 2006 Feb . 9(2):283-91.

    Insert

    H-Ras (HRAS Human)
    

    Use

    Tags

    monomeric EGFP

    Expression

    Mammalian

    Mutation

    G12V mutation in HRas

    Promoter

    Available since

    July 2, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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15. piggyBAC-H2B FR-MQV

    Plasmid

    #194079

    Purpose

    Expresses nuclear-localized FR-MQV in mammalian cells

    Depositor

    Ralph Jimenez

    Article

    Mukherjee et al Biochemistry. 2020 Oct 6;59(39):3669-368

    Insert

    FR-MQV
    

    Use

    Tags

    Histone H2B

    Expression

    Mammalian

    Mutation

    Methionine 42 to Glutamine, Cystiene 159 to Valin…

    Promoter

    CMV7

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. GST-BHMT-I/G

    Plasmid

    #104442

    Purpose

    Autophagy cargo assay

    Depositor

    Carol Mercer

    Article

    Mercer et al Autophagy. 2008 Feb;4(2):185-94. Epub 20

    Insert

    BHMT (BHMT Human)
    

    Use

    Tags

    GFP-myc GFP is from Aequorea victoria and GST

    Expression

    Mammalian

    Mutation

    N43A in GST (in a potential KFERQ-like sequence).…

    Promoter

    CMV

    Available since

    Dec. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. ErkKTR-irFP

    Plasmid

    #111510

    Purpose

    Reporter of Erk activity

    Depositor

    Jared Toettcher

    Article

    Dine et al Cell Syst. 2018 May 24. pii: S2405-4712(

    Insert

    ERK KTR (Mapk1 Mouse)
    

    Use

    Lentiviral

    Tags

    iRFP

    Expression

    Mutation

    Promoter

    SSFV

    Available since

    June 14, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3.1-hACE2-LgBiT

    Plasmid

    #215837

    Purpose

    Expresses hACE2 receptor with LgBiT tag in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    hACE2-LgBiT (ACE2 )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRK5-HA-Sestrin2

    Plasmid

    #72593

    Purpose

    overexpression, codon optimized for mammalian expression

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell Rep. 2014 Oct 9;9(1):1-8. doi: 10.1

    Insert

    SESN2 (SESN2 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    codon optimized for mammalian expression, see dep…

    Promoter

    CMV

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pCDH-TfR-mApple

    Plasmid

    #179384

    Purpose

    Lentiviral vector for expression of Human Transferrin receptor-1 fused mApple

    Depositor

    Angus Johnston

    Article

    Rennick et al Nat Commun. 2022 Oct 12;13(1):6023. doi:

    Insert

    Transferrin receptor 1 - mApple (TFRC Human, Synthetic)
    

    Use

    Lentiviral

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    EF1

    Available since

    Oct. 11, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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