We narrowed to 9,854 results for: ada

1. Donor-PIGA

   Plasmid

   #153530

   Purpose

   Used as a donor vector for the targeted correction of a mutation in PIGA exon 6

   Depositor

   Hiroyuki Konishi

   Article

   Hyodo et al Cell Rep. 2020 Jan 28;30(4):1195-1207.e7

   Insert

   PIGA (a 1,991-bp fragment from intron 5 and exon 6)
   

   Use

   Donor plasmid

   Tags

   N/A

   Expression

   Mutation

   No mutation

   Promoter

   No promoter

   Available since

   Dec. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pEGFP-N1 gp78fl

   Plasmid

   #37375

   Depositor

   Allan Weissman

   Article

   Fang et al Proc Natl Acad Sci U S A. 2001 Dec 4. 98

   Insert

   gp78 (AMFR Human)
   

   Use

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   P179L and F262L

   Promoter

   Available since

   Nov. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pet42c(+)-GST-USP5

   Plasmid

   #61750

   Purpose

   express N-terminally GST tagged human USP5

   Depositor

   Yihong Ye

   Article

   Liu et al Elife. 2014;3:e01369. doi: 10.7554/eLife

   Insert

   USP5 (USP5 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Feb. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pCHC039 (∆phcA)

   Plasmid

   #226207

   Purpose

   Conjugation knockout plasmid (pK18msB) to delete the C. necator LysR-type transcriptional regulator PhcA.

   Depositor

   Christopher Johnson

   Article

   Calvey et al Metab Eng. 2023 Jan;75:78-90. doi: 10.10

   Insert

   Homology arms for PhcA
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Nov. 18, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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5. pHLP712

   Plasmid

   #203741

   Purpose

   Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus

   Depositor

   Mark Hall

   Article

   Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

   Insert

   OsTIR1
   

   Use

   Candida albicans integrating vector

   Tags

   3xMyc

   Expression

   Mutation

   Codon 74 changed from Phe to Ala (F74A)

   Promoter

   ACT1 (C. albicans)

   Available since

   Aug. 16, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pEF-Bos MAL Flag

   Plasmid

   #41554

   Depositor

   Kate Fitzgerald, Tom Maniatis

   Article

   Fitzgerald et al Nat Immunol. 2003 May;4(5):491-6.

   Insert

   MAL (TIRAP Human)
   

   Use

   Tags

   6xHis and Flag

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Feb. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

7. Rap1A FLARE

   Plasmid

   #134926

   Purpose

   Rap1A biosensor

   Depositor

   Klaus Hahn

   Article

   O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

   Insert

   Rap1A, mCerulean3, RaIGDS RBD, YPet
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTK331

   Plasmid

   #59572

   Purpose

   TC-83 I-SceI T7 5'UTR (A3G) P1234 (nsP2 Q739L) SGP(14) Kozak mKate-G8-L7Ae-PEST 4xFF4 attB2 SGP2(98/30) XbaI attB1 EBFP2 ochre attB2 (insert) AscI (truncated E1) 3'UTR Poly A I-SceI

   Depositor

   Ron Weiss

   Article

   Wroblewska et al Nat Biotechnol. 2015 Aug 3. doi: 10.1038

   Insert

   SGP(16) 2xK-turn Kozak EGFP attB2 AscI
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 3, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-ALK5 K232R

   Plasmid

   #80878

   Purpose

   mammalian expression of ALK5 K232R

   Depositor

   Aristidis Moustakas

   Article

   Moren et al J Biol Chem. 2005 Jun 10;280(23):22115-2

   Insert

   ALK5 (TGFBR1 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K232R (kinase dead)

   Promoter

   CMV

   Available since

   Aug. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. MAC_N_ETV6

    Plasmid

    #167755

    Purpose

    Expresses ETV6 in mammalian cells

    Depositor

    Markku Varjosalo

    Article

    Goos et al Nat Commun. 2022 Feb 9;13(1):766. doi: 1

    Insert

    ETV6 (EN2 Human)
    

    Use

    Tags

    MAC

    Expression

    Mammalian

    Mutation

    no mutations

    Promoter

    Available since

    Jan. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Rap1B FLARE

    Plasmid

    #134927

    Purpose

    Rap1B biosensor

    Depositor

    Klaus Hahn

    Article

    O'Shaughnessy et al J Cell Biol. 2019 Sep 2;218(9):3153-3160

    Insert

    RaIGDS RBD, YPet, Cerulean, Rap1B
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCFJ1410 - [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)

    Plasmid

    #159859

    Purpose

    Three-fragment Gateway compatible flourophore for expression in C. elegans

    Depositor

    Christian Froekjaer-Jensen

    Article

    Aljohani et al Nat Commun. 2020 Dec 9;11(1):6300. doi:

    Insert

    [1-2] ENTR - Fluor - mCherry(PATCs, NLS(2), no_atg, no_stop)
    

    Use

    Tags

    Expression

    Worm

    Mutation

    Not applicable

    Promoter

    Available since

    Dec. 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pIBA3-SpyCatcher-sfGFP

    Plasmid

    #107420

    Purpose

    For production of SpyCatcher protein fused N-terminally to sfGFP. Includes N-terminal His tag for purification and C-terminal StrepII tag for detection.

    Depositor

    Jack Leo

    Article

    Chauhan et al Mol Microbiol. 2019 Jan 1. doi: 10.1111/

    Insert

    SpyCatcher
    

    Use

    Tags

    His tag, StrepII, and superfolder GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Tet

    Available since

    Jan. 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCOM-CD63-COM

    Plasmid

    #138350

    Purpose

    Expressing COM-CD63-COM fusion protein in mammalian cells

    Depositor

    Baisong Lu

    Article

    Yao et al J Extracell Vesicles. 2021 Mar;10(5):e12

    Insert

    COM-CD63-COM fusion protein
    

    Use

    CRISPR and Lentiviral

    Tags

    COM is fused to the N and C termini of human CD63

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pTW072M

    Plasmid

    #115960

    Purpose

    Destination Vector

    Depositor

    Ron Weiss

    Article

    Wagner et al Nat Chem Biol. 2018 Nov;14(11):1043-1050

    Insert

    Lvl2 (nsP2 Q739L)
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mutation

    nsP2 Q739L

    Promoter

    Available since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. ABE8e-dimer

    Plasmid

    #138490

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(WT)-ecTadA(8e)-nSpCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAG-ChRmine-mRuby2-ST

    Plasmid

    #227777

    Purpose

    Cation channelrhodopsin ChRmine fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

    Depositor

    Hillel Adesnik

    Article

    Sridharan et al Neuron. 2022 Jan 27. pii: S0896-6273(22)

    Insert

    ChRmine-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pcDNA3-KLF5-FLAG

    Plasmid

    #40917

    Depositor

    Jin-Tang Dong

    Article

    Zhao et al Biochem J. 2011 Jul 15;437(2):323-33.

    Insert

    Kruppel-like factor 5 (KLF5 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 6, 2012

    Availability

    Academic Institutions and Nonprofits only

19. pCS2+_HA-mAID-nanobody (Kless)

    Plasmid

    #117718

    Purpose

    mRNA synthesis of lysine-less HA-mAID-nanobdy for injection

    Depositor

    Joerg Mansfeld

    Article

    Daniel et al Nat Commun. 2018 Aug 17;9(1):3297. doi:

    Insert

    mAID-nanobody
    

    Use

    Tags

    3xHA

    Expression

    Mammalian

    Mutation

    codon-optimized and lysine-less mAID for murine e…

    Promoter

    SP6

    Available since

    Dec. 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCRISPRi

    Plasmid

    #199808

    Purpose

    vector for constitutive CRISPRi-mediated gene knockdown in S. aureus

    Depositor

    Steve Salipante

    Article

    Long et al PLoS Pathog. 2024 Jul 11;20(7):e1012394.

    Inserts

    dCas9
    sgRNA with GFP cassette
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    P23 and PRAB17 (sgRNA) and SarAP1 (GFP)

    Available since

    July 31, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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