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DUSP6

Plasmid

#27975

Depositor

Igor Astsaturov

Article

Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

Insert

DUSP6 (DUSP6 Human)

Use

Lentiviral

Tags

HA and Myc

Expression

Mammalian

Promoter

CMV

Available Since

April 8, 2011

Availability

Academic Institutions and Nonprofits only

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Pet22b-mEGFP

Plasmid

#166439

Purpose

Bacterial expression of 6x His tagged mEGFP

Depositor

Shuguo Sun

Article

Yu et al Mol Cell. 2021 Feb 13. pii: S1097-2765(2

Insert

mEGFP

Expression

Bacterial

Available Since

May 12, 2021

Availability

Academic Institutions and Nonprofits only

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pPgiCas13b

Plasmid

#184833

Purpose

Inducible expression of PgiCas13b nuclease in bacteria.

Depositor

Chase Beisel

Article

Wandera et al Mol Cell. 2022 May 24. pii: S1097-2765(2

Insert

Cas13b nuclease from Porphyromonas gingivalis

Use

CRISPR

Expression

Bacterial

Promoter

T7 promoter

Available Since

Nov. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pSFFV-dNme2Cas9

Plasmid

#193190

Purpose

Lentiviral vector expressing nuclease-dead Cas9 from Neisseria meningitis (dNme2)

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

dNme2Cas9

Tags

NLS and NLS-3xHA-NLS

Expression

Mammalian

Mutation

H588A, D16A

Promoter

SFFV

Available Since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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∆DUSP6

Plasmid

#27977

Depositor

Igor Astsaturov

Article

Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

Insert

DUSP6 (DUSP6 Human)

Use

Lentiviral

Tags

HA and MYC

Expression

Mammalian

Mutation

C293S: mutation in the phosphatase domain

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only
pSLQ9834_sglacZ: pHR-hU6-CasMINI sgRNA_#2 (sgLacZ); EF1a-Puro-T2A-BFP- WPRE

Plasmid

#176272

Purpose

Expression of non-targeting sgRNA of CasMINI

Depositor

Stanley Qi

Article

Xu et al Mol Cell. 2021 Aug 26. pii: S1097-2765(2

Insert

CasMINI non-targeting sgRNA (sgLacZ) and BFP

Use

Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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mCherry-CRY2-5ptaseOCRL

Plasmid

#66836

Purpose

Expression of inositol 5-phosphatase domain of OCRL. Binds to CIBN upon blue light illumination.

Depositor

Pietro De Camilli

Article

Idevall-Hagren et al Proc Natl Acad Sci U S A. 2012 Aug 28;10

Insert

5ptaseOCRL (OCRL Human)

Tags

mCherry-CRY2

Expression

Mammalian

Promoter

CMV

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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p306-BrdU-Inc

Plasmid

#71792

Purpose

BrdU-incorporating plasmid with selectable marker URA3

Depositor

Oscar Aparicio

Article

Viggiani et al Yeast. 2006 Oct-Nov;23(14-15):1045-51.

Inserts

Thymidine Kinase
ENT1 (SLC29A1 Human)

Expression

Yeast

Promoter

ADH1 and GPD

Available Since

Feb. 17, 2016

Availability

Academic Institutions and Nonprofits only

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mTagRFP-T-Mito-7

Plasmid

#58023

Purpose

Localization: Mitochondria, Excitation: 555, Emission: 584

Depositor

Michael Davidson

Article

Day et al Chem Soc Rev. 2009 Oct;38(10):2887-921.

Insert

Mito (COX8A Human)

Tags

mTagRFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 4, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pET28a-MH6-EsCas13d

Plasmid

#108303

Purpose

Expresses E. coli codon optimized EsCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

EsCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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PLKO.1-G3BP1-CDS

Plasmid

#136039

Purpose

G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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SAP145

Plasmid

#11239

Depositor

Robin Reed

Article

Gozani et al Genes Dev. 1996 Jan 15. 10(2):233-43.

Insert

SAP145 (SF3B2 Human)

Expression

Bacterial

Mutation

First 7 amino acids are missing compared to NP_00…

Available Since

Jan. 19, 2006

Availability

Academic Institutions and Nonprofits only

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Beta-Globin-WT

Plasmid

#130700

Purpose

Expresses Beta-Globin WT NMD reporter

Depositor

Marvin Tanenbaum

Article

Hoek et al Mol Cell. 2019 May 23. pii: S1097-2765(1

Insert

Beta Globin (HBB Human)

Expression

Mammalian

Promoter

Pcmv

Available Since

Sept. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pN256

Plasmid

#188472

Purpose

scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

Depositor

Le Cong

Article

Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

Insert

eGFP-WPRE-DAISY-4T

Available Since

Sept. 6, 2022

Availability

Academic Institutions and Nonprofits only

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pET28a-MH6-RspCas13d

Plasmid

#108305

Purpose

Expresses E. coli codon optimized RspCas13d in the pET28a backbone.

Depositor

Arbor Biotechnologies

Article

Yan et al Mol Cell. 2018 Mar 9. pii: S1097-2765(18

Insert

RspCas13d

Use

CRISPR

Tags

mH6 tag

Expression

Bacterial

Promoter

lac

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJW1512

Plasmid

#62366

Purpose

N-terminal 3X FLAG fused to wt BirA - mCherry - sec61ß

Depositor

Jonathan Weissman

Article

Jan et al Science. 2014 Nov 7;346(6210):1257521. d

Insert

3xFLAG-BirA-mCherry-Sec61beta tail anchor (SEC61B Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pTRE2

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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Nme2Cas9_pCDest

Plasmid

#119923

Purpose

Nme2Cas9 CMV-driven mammalian expression plasmid

Depositor

Erik Sontheimer

Article

Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

Insert

Nme2Cas9

Tags

NLS-3xHA-NLS

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 18, 2019

Availability

Academic Institutions and Nonprofits only

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RA-Sec61β

Plasmid

#153978

Purpose

Dimerization dependent fluorescent protein RA domain fused to ER protein Sec61β

Depositor

Gia Voeltz

Article

Lee et al Science. 2020 Jan 31;367(6477). pii: 367

Insert

Sec61 Transolocon Subunit Beta (SEC61B Human)

Tags

pAcGFP-C1

Expression

Mammalian

Available Since

July 21, 2020

Availability

Academic Institutions and Nonprofits only

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pCS2-3HA-Mefv iso1

Plasmid

#92105

Purpose

Expression in eukaryotic cells

Depositor

Feng Shao

Article

Gao et al Proc Natl Acad Sci U S A. 2016 Aug 16;11

Insert

Mefv isoform1 (Mefv Mouse)

Tags

3xHA

Expression

Mammalian

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only
3xFLAG-SENP7

Plasmid

#42886

Depositor

Edward Yeh

Article

Bawa-Khalfe et al Proc Natl Acad Sci U S A. 2012 Oct 23;10

Insert

SENP7 (SENP7 Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

T102A, R969C, L996S

Available Since

April 23, 2013

Availability

Academic Institutions and Nonprofits only

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