We narrowed to 11,240 results for: AGA

Showing: 3741 - 3760 of 11240 results

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pRK5_mCherry_P2AT2A_GFP-nb-KS

Plasmid

#238233

Purpose

For overexpression of mCherry_P2AT2A_GFP-nb-KS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-KS

Expression

Mammalian

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-6085x

Plasmid

#108653

Purpose

A tyrosine kinase biosensor based on fluorescence resonance energy transfer (FRET) and those based on bioluminescence energy transfer (BRET)

Depositor

Michiyuki Matsuda

Article

Komatsu et al Sci Rep. 2018 Jun 12;8(1):8984. doi: 10.

Insert

PicchuEV

Use

Lentiviral

Tags

XFP

Expression

Mammalian

Available Since

April 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCASB

Plasmid

#190175

Purpose

Plasmid for yeast expression of Cas9; contains a golden gate cloning site to additionally express a gRNA for a target sequence

Depositor

Jessica Tyler

Article

Aguilar et al Methods Protoc. 2022 Oct 4;5(5):79. doi:

Insert

sgRNA cloning site

Expression

Bacterial and Yeast

Mutation

Added 5'-agagacc-3' upstream and 5'…

Available Since

Oct. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pMK419 (GAL-OsTIR1(F74G))

Plasmid

#140656

Purpose

GAL-OsTIR1(F74G)

Depositor

Masato Kanemaki

Article

Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

Insert

GAL-OsTIR1(F74G)

Expression

Yeast

Available Since

Nov. 12, 2020

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck3

Plasmid

#136136

Purpose

L1 in position 3, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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HDAC5 Flag

Plasmid

#13822

Purpose

Mammalian expression of human histone deacetylase 5 with flag tag

Depositor

Eric Verdin

Article

Fischle et al J Biol Chem. 1999 Apr 23. 274(17):11713-

Insert

HDAC5 (HDAC5 Human)

Tags

flag

Expression

Mammalian

Available Since

Feb. 19, 2007

Availability

Academic Institutions and Nonprofits only

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L1_lacZgRNA-Ck2

Plasmid

#136137

Purpose

L1 in position 2, to clone gRNA target using BbsI, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

p5-MpU6:lacZgRNA

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Academic Institutions and Nonprofits only

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nlsSalicS1

Plasmid

#232190

Purpose

Novel FRET-based biosensor for salicylic acid

Depositor

Alexander Jones

Article

Tang et al Science. 2025 Oct 9;390(6769):188-194. d

Insert

nlsSalicS1

Tags

His-tag

Expression

Plant

Promoter

p16

Available Since

Oct. 10, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9n(BB)-2A-Puro (PX462)

Plasmid

#48141

Purpose

NOTE: A new version of this plasmid is now available. See Addgene plasmid 62987

Depositor

Feng Zhang

Article

Ran et al Nat Protoc. 2013 Nov;8(11):2281-308. doi

Insert

hSpCas9n-2A-Puro

Use

CRISPR

Tags

2A-Puro and 3XFLAG

Expression

Mammalian

Mutation

Cas9 D10A nickase mutant

Promoter

Cbh

Available Since

Oct. 29, 2013

Availability

Academic Institutions and Nonprofits only
pTorPE-B-GECO1

Plasmid

#32469

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

B-GECO1.0

Use

Lab constructed

Tags

6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…

Expression

Bacterial

Mutation

GCaMP3 S40P/L60P/D86G/K119I/L173Q/T223S/Y224H/D30…

Available Since

Oct. 14, 2011

Availability

Academic Institutions and Nonprofits only

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PB533-42B3mutAC2-sfGFP

Plasmid

#186777

Purpose

RNAP2 Ser2ph-mintbody [42B3(R78K/A80T/M95V)-scFv] expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Uchino et al J Cell Biol. 2022 Feb 7;221(2). pii: 212

Insert

42B3mutAC2-scFv

Tags

sfGFP

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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OR52-consensus

Plasmid

#208080

Purpose

Consensus OR52 engineered from human OR52 family members

Depositor

Hiroaki Matsunami

Article

Ikegami et al Proc Natl Acad Sci U S A. 2020 Feb 11;11

Insert

OR52-consensus

Tags

Rhodopsin tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-G-GECO1.2

Plasmid

#32446

Purpose

Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.2)

Depositor

Robert Campbell

Article

Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

Insert

G-GECO1.2

Expression

Mammalian

Mutation

GCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…

Promoter

CMV

Available Since

Sept. 29, 2011

Availability

Academic Institutions and Nonprofits only

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pLKO.1-TetON-Puro-HIF1a 3809

Plasmid

#118705

Purpose

Lentivirus for expression of shRNA1 against human HIF1a (Dox-inducible)

Depositor

William Kaelin

Article

Briggs et al Cell. 2016 Jun 30;166(1):126-39. doi: 10

Insert

shRNA against human HIF1a

Use

Lentiviral

Available Since

Nov. 8, 2018

Availability

Academic Institutions and Nonprofits only

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PB_TetON_mCherry_P2AT2A_GFP-nb-2xKS

Plasmid

#238243

Purpose

For integration of mCherry_P2AT2A_GFP-nb-2xKS

Depositor

Denes Hnisz

Article

Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

Insert

mCherry_P2AT2A_GFP-nb-2xKS

Expression

Mammalian

Available Since

June 4, 2025

Availability

Academic Institutions and Nonprofits only

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pMK287 (mAID-Hygro)

Plasmid

#72825

Purpose

C-terminal tagging with mAID using CRISPR/Cas9

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

mAID-Hygro

Use

CRISPR

Tags

mAID

Expression

Mammalian

Available Since

Feb. 23, 2016

Availability

Academic Institutions and Nonprofits only

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pISCba-reRNA

Plasmid

#226823

Purpose

Expressing ISCba controled by PlacM promoter, guide RNA controled byPJ23119 promoter

Depositor

Sheng Yang

Article

Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

Insert

ISCba and its corresponding guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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pISClsp3-reRNA

Plasmid

#226824

Purpose

Expressing ISClsp3 controled by PlacM promoter, guide RNA controled by PJ23119 promoter

Depositor

Sheng Yang

Article

Wang et al ACS Synth Biol. 2025 May 8. doi: 10.1021

Insert

ISClsp3 and its corresponding guide RNA

Expression

Bacterial

Available Since

March 27, 2025

Availability

Academic Institutions and Nonprofits only

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PLKO.1-G3BP1-3'UTR

Plasmid

#136038

Purpose

G3BP1 shRNA (Targeting 3'UTR) inserted into the PLKO.1 plasmid (GCCTGTAAGAAATACAGGATT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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PLKO.1-G3BP1-CDS

Plasmid

#136039

Purpose

G3BP1 shRNA (Targeting CDS) inserted into the PLKO.1 plasmid (CGGGAATTTGTGAGACAGTAT)

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

G3BP1 (G3BP1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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