We narrowed to 4,337 results for: U6 gRNA

1. pBPK1520-SNCA-A53T-ng

   Plasmid

   #181739

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A53T mutation in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for SNCA-A53T (SNCA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pTE4889

   Plasmid

   #88905

   Purpose

   Expresses FnCpf1 crRNA and inactive/dead, humanized FnCpf1 nuclease

   Depositor

   Ervin Welker

   Article

   Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

   Inserts

   Fn crRNA
   hFnCpf1(D917A)
   

   Use

   CRISPR

   Tags

   3xHA and NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV and human U6

   Available since

   April 14, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLV-shEsr2

   Plasmid

   #120722

   Purpose

   Lentiviral vector that expresses GFP and an shRNA targeting Esr2 (in pLL3.7).

   Depositor

   Amy Lasek

   Article

   Vandegrift et al J Neurosci. 2020 Jul 1;40(27):5196-5207.

   Insert

   Esr2 (Esr2 Mouse)
   

   Use

   Lentiviral and RNAi

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   mouse U6

   Available since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. BE3-hA3A-R128A

   Plasmid

   #132944

   Purpose

   Gene editing. See Gene/Insert section of the plasmid page for targeting sequence cloned into this plasmid.

   Depositor

   Hui Yang

   Article

   Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

   Insert

   BE3-hA3A(R128A)
   

   Use

   CRISPR; Base editor

   Tags

   Expression

   Mammalian

   Mutation

   BE3-hA3A(R128A)

   Promoter

   Available since

   Nov. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLKO_HOXB13_#2

   Plasmid

   #70094

   Purpose

   Expression of shRNA to human HOXB13, puromycin selection

   Depositor

   William Hahn

   Article

   Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

6. pLKO_HOXB13_#1

   Plasmid

   #70093

   Purpose

   Expression of shRNA to human HOXB13, puromycin selection

   Depositor

   William Hahn

   Article

   Pomerantz et al Nat Genet. 2015 Nov;47(11):1346-51. doi:

   Insert

   HOXB13 (HOXB13 Human)
   

   Use

   Lentiviral and RNAi

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 6, 2015

   Availability

   Academic Institutions and Nonprofits only

7. pBPK1520-SNCA-A30P-ng

   Plasmid

   #180018

   Purpose

   Transiently expressing a nicking sgRNA to facilitate the introduction of SNCA-A30P mutation in PE3 approach

   Depositor

   Dirk Hockemeyer

   Article

   Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

   Insert

   Prime editing ngRNA for SNCA-A30P (SNCA Human)
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Human U6

   Available since

   Sept. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Lenti_CRISPRi_sgAR2

   Plasmid

   #167001

   Purpose

   Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   AR Promoter sgRNA 2 (AR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Lenti_CRISPRi_sgAR1

   Plasmid

   #167000

   Purpose

   Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

   Depositor

   Gian-Paolo Dotto

   Article

   Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

   Insert

   AR Promoter sgRNA 1 (AR Human)
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   U6

   Available since

   April 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. lentiCRISPR v2-sgCOQ2

    Plasmid

    #186025

    Purpose

    knock out COQ2 in mammalian cells

    Depositor

    Boyi Gan

    Article

    Mao et al Nature. 2021 May;593(7860):586-590. doi:

    Insert

    Coenzyme Q2, Polyprenyltransferase (COQ2 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    June 21, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. BE3-hA3A-Y130F

    Plasmid

    #132945

    Purpose

    Gene editing. See Gene/Insert section of plasmid page for targeting sequence cloned into this plasmid.

    Depositor

    Hui Yang

    Article

    Zhou et al Nature. 2019 Jul;571(7764):275-278. doi:

    Insert

    BE3-hA3A(Y130F)
    

    Use

    CRISPR; Base editor

    Tags

    Expression

    Mammalian

    Mutation

    BE3-hA3A(Y130F)

    Promoter

    Available since

    Nov. 24, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSico shSMYD3-C human

    Plasmid

    #85654

    Purpose

    expression of shRNA targeting human SMYD3

    Depositor

    Julien Sage

    Article

    Mazur et al Nature. 2014 Jun 12;510(7504):283-7. doi

    Insert

    shRNA targeting 3'UTR of SMYD3 (SMYD3 Human)
    

    Use

    Lentiviral and RNAi

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti_CRISPRi_sgAR3

    Plasmid

    #167002

    Purpose

    Lentiviral expression of sgRNA targeted to the androgen receptor (AR) promoter for CRISPRi knockdown

    Depositor

    Gian-Paolo Dotto

    Article

    Ma et al J Exp Med. 2021 Feb 1;218(2). pii: 21150

    Insert

    AR Promoter sgRNA 3 (AR Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    April 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pTE4566

    Plasmid

    #88904

    Purpose

    Expresses MbCpf1 crRNA and inactive/dead, humanized MbCpf1 nuclease

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    hMbCpf1(D986A)
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV and human U6

    Available since

    April 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Lenti-sgNotch2#2/Cre

    Plasmid

    #193226

    Purpose

    Lentiviral vector expressing Cre recombinase (driven by mPGK promoter) and an sgRNA against the mouse Notch2 gene

    Depositor

    Julien Sage

    Article

    Lee et al Cell Rep. 2023 Jan 13;42(1):111990. doi:

    Insert

    sgNotch2#2 (Notch2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pTE3328

    Plasmid

    #107539

    Purpose

    Expresses As crRNA and human codon optimized AsCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    As crRNA
    hAsCpf1(RR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    S542R, K607R

    Promoter

    CMV and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pTE3329

    Plasmid

    #107538

    Purpose

    Expresses Lb crRNA and human codon optimized LbCpf1(RR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Lb crRNA
    hLbCpf1(RR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    G532R, K595R

    Promoter

    CMV and human U6

    Available since

    March 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pTE3327

    Plasmid

    #107535

    Purpose

    Expresses As crRNA and human codon optimized AsCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    As crRNA
    hAsCpf1(RVR mutant)
    

    Use

    Tags

    3xHA, NLS, and SV40 NLS

    Expression

    Mammalian

    Mutation

    S542R, K548V, N552R

    Promoter

    CMV and human U6

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEN_hU6miR-G12-E

    Plasmid

    #25757

    Purpose

    Entry vector with human U6 promoter driving mouse G alpha 12 miR30-based shRNA.

    Depositor

    Iain Fraser

    Article

    Shin et al Proc Natl Acad Sci U S A. 2006 Sep 12. 1

    Insert

    G alpha 12 miR-shRNA (Gna12 Mouse)
    

    Use

    Entry vector

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 14, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pTE3334

    Plasmid

    #107537

    Purpose

    Expresses Mb crRNA and human codon optimized MbCpf1(RVR mutant) in mammalian cells.

    Depositor

    Ervin Welker

    Article

    Toth et al Nucleic Acids Res. 2018 Sep 20. pii: 510

    Inserts

    Mb crRNA
    hMbCpf1(RVR mutant)
    

    Use

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    N576R, K582V, N586R

    Promoter

    CMV and human U6

    Available since

    March 30, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge