4,181 results

1. Raptor_1 shRNA

   Plasmid

   #1857

   Depositor

   David Sabatini

   Article

   Sarbassov et al Science 2005 Feb 18;307(5712):1098-101.

   Insert

   Raptor (RPTOR Human)
   

   Use

   Lentiviral and RNAi

   Expression

   Mammalian

   Available Since

   April 5, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX330A-FBL/PITCh

   Plasmid

   #63671

   Purpose

   Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

   Depositor

   Takashi Yamamoto

   Article

   Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

   Insert

   humanized S. pyogenes Cas9
   

   Use

   CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Promoter

   CBh

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

3. pTP1183

   Plasmid

   #104163

   Purpose

   Bacterial expression plasmid of anti-rabbit IgG Fc nanobody TP897 (1x Cysteine)

   Depositor

   Dirk Görlich

   Article

   Pleiner et al J Cell Biol. 2018 Mar 5;217(3):1143-1154

   Insert

   Anti-rabbit IgG Fc specific nanobody TP897 (1xCysteine)
   

   Tags

   14xHistidine tag and NEDD8 from Brachypodium dist…

   Expression

   Bacterial

   Available Since

   Dec. 21, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBS-L1PA1-CH-mneo

   Plasmid

   #51288

   Purpose

   contains the fully codon optimized ORF1 and ORF2 of the human L1RP

   Depositor

   Astrid Roy-Engel

   Article

   Wagstaff et al PLoS One. 2011 May 10;6(5):e19672. doi:

   Insert

   L1 (L1RE1 Human)
   

   Tags

   mneoI indicator cassette containing an inverted n…

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLBH531_MBP-Cas14a1 expression

   Plasmid

   #112500

   Purpose

   Expresses MBP fused to Cas14a1

   Depositor

   Jennifer Doudna

   Article

   Harrington et al Science. 2018 Oct 18. pii: science.aav42

   Insert

   Cas14a1
   

   Use

   CRISPR

   Tags

   10xHis-MBP

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Oct. 26, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. dCas9-hHDAC3

   Plasmid

   #98591

   Purpose

   3rd generation lenti vector encoding dCas9-hHDAC3 (EF1a-NLS-dCas9(N863)-hHDAC3). Expresses dCas9 fused to human HDAC3.

   Depositor

   Zhaolan Zhou

   Article

   Kwon et al Nat Commun. 2017 May 12;8:15315. doi: 10

   Insert

   dCAS9-HDAC3 (HDAC3 Human, S. pyogenes, Synthetic)
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   EF1A

   Available Since

   Oct. 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pZR071_SFFV-dCas9-mCherry-KRAB

   Plasmid

   #180264

   Purpose

   Lentiviral expression vector for dCas9-KRAB in mammalian cells

   Depositor

   Alexander Marson

   Article

   Schmidt et al Science. 2022 Feb 4;375(6580):eabj4008.

   Insert

   dCas9
   

   Use

   CRISPR and Lentiviral

   Tags

   KRAB and mCherry

   Expression

   Mammalian

   Mutation

   Nuclease dead Cas9

   Promoter

   SFFV

   Available Since

   Feb. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA3-Tet1

   Plasmid

   #60938

   Purpose

   Expresses Tet1 in mammalian cells

   Depositor

   Yi Zhang

   Article

   Wang et al Cell Rep. 2014 Jan 30;6(2):278-84. doi:

   Insert

   Ten-Eleven Translocation 1 (Tet1 Mouse)
   

   Tags

   flag

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 10, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-CMV-GFP

   Plasmid

   #67634

   Purpose

   AAV vector expressing GFP

   Depositor

   Connie Cepko

   Article

   Xiong et al J Clin Invest. 2015 Apr;125(4):1433-45.

   Insert

   GFP
   

   Use

   AAV

   Promoter

   CMV

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. Nme2Cas9_AAV

    Plasmid

    #119924

    Purpose

    All-in-one AAV plasmid expressing Nme2Cas9 with sgRNA cassette

    Depositor

    Erik Sontheimer

    Article

    Edraki et al Mol Cell. 2018 Dec 18. pii: S1097-2765(1

    Insert

    Nme2Cas9
    

    Use

    AAV

    Tags

    NLS-3xHA-NLS and NLS-NLS

    Expression

    Mammalian

    Promoter

    U1a

    Available Since

    Jan. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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