We narrowed to 9,202 results for: sacs

1. pFW2000

   Plasmid

   #224211

   Purpose

   E. coli-Bacteroides shuttle plasmid; pB8-51 (Bacteroides replication origin), p15A (E. coli replication origin)

   Depositor

   Ophelia Venturelli

   Article

   Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

   Type

   Empty backbone

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Sept. 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. p415TEF cyto roGFP2-Grx2(yeast)

   Plasmid

   #65005

   Purpose

   Yeast expression plasmid for roGFP2-Grx2; yeast codon optimized roGFP; yeast Grx2

   Depositor

   Tobias Dick

   Article

   Morgan et al Nat Chem Biol. 2013 Feb;9(2):119-25. doi

   Insert

   GRX2
   

   Use

   Tags

   roGFP2

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   June 29, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pPMVAu8

   Plasmid

   #98297

   Purpose

   Enhancing the upper-part mevalonate pathway; overexpressing codon-optimized Enterococcus faecalis mevalonate pathway genes under the control of consitutive promoters.

   Depositor

   Claudia Vickers

   Article

   Peng et al Metab Eng. 2017 Jan;39:209-219. doi: 10.

   Insert

   PRPL4A-EfmvaS-TEFM1-PRPL15A-EfmvaE -TEBS1
   

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 7, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. OA-1053B

   Plasmid

   #184007

   Purpose

   Expresses arrays of gRNA targeting hh promoter under U6b/c promoter

   Depositor

   Omar Akbari

   Article

   Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

   Insert

   U6b:sgRNAhh1 - U6c:sgRNAhh2
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCRISPEY-Z3-Nat

   Plasmid

   #232100

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of CRISPR guide RNA and repair template for homologous recombination, with Z3 promoter and Z3EV transcription factor.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Feb. 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCas9-EcRT-Z3-Hyg

   Plasmid

   #232095

   Purpose

   Yeast CEN plasmid for estradiol-inducible expression of spCas9 and Ec86 reverse transcriptase.

   Depositor

   Jeffrey Lewis

   Article

   Stuecker et al G3 (Bethesda). 2025 Aug 4:jkaf175. doi:

   Insert

   Z3 promoter and Z3EV transcription factor
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Z3

   Available since

   Feb. 20, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBPGAL4.2::p65Uw

   Plasmid

   #26229

   Depositor

   Gerald Rubin

   Article

   Pfeiffer et al Genetics. 2010 Oct;186(2):735-55. doi: 1

   Insert

   GAL4::p65 (RELA Budding Yeast, Human)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Oct. 22, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTH847-gst-optsc

   Plasmid

   #96908

   Purpose

   Plasmid for yeast expression of GST encoded by preferred codons

   Depositor

   Tobias von der Haar

   Article

   Jossé et al bioRxiv 200006

   Insert

   yeast codon optimised glutathione-S transferase
   

   Use

   Tags

   8xhistidine

   Expression

   Yeast

   Mutation

   Promoter

   TDH3

   Available since

   March 20, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. LT2_12_GlyRNA

   Plasmid

   #177710

   Purpose

   Plasmid for genome integration in X2 site expressing GlyRNA (glycolytic flux biosensor)

   Depositor

   Lisbeth Olsson

   Article

   Torello Pianale et al Front Microbiol. 2022 Jan 7;12:802169. d

   Inserts

   pTEFmut8-mTurquoise2-RNA_2.6--tCYC1
   pTEFmut8-mCherry-tADH1
   

   Use

   Used as donor dna in genome integration after lin…

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Jan. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pFW2100

    Plasmid

    #224212

    Purpose

    Bacteroides genomic editing tool; IPTG inducible-Fncpf1

    Depositor

    Ophelia Venturelli

    Article

    Feng et al Cell Host Microbe. 2022 Feb 9;30(2):200-

    Insert

    Fncpf1
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pEx-insertion-erm

    Plasmid

    #172210

    Purpose

    For genetic insertion in erythromycin-sensitive Prevotella copri strains

    Depositor

    Till Strowig

    Article

    Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

    Type

    Empty backbone

    Use

    Genetic insertion in prevotella copri

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEx-insertion-erm-T1T2

    Plasmid

    #172212

    Purpose

    For genetic insertion in erythromycin-sensitive Prevotella copri strains, with T1-T2 terminator

    Depositor

    Till Strowig

    Article

    Li et al EMBO J. 2021 Dec 1;40(23):e108287. doi:

    Type

    Empty backbone

    Use

    Genetic insertion in prevotella copri

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. OA-1053A

    Plasmid

    #184006

    Purpose

    Expresses arrays of gRNA targeting eve promoter under U6b/c promoter

    Depositor

    Omar Akbari

    Article

    Bui et al PLoS Pathog. 2023 Jan 19;19(1):e1010842.

    Insert

    U6b:sgRNAeve1 - U6c:sgRNAeve2
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-Cas9-162A

    Plasmid

    #84918

    Purpose

    for in vitro transcription of Cas9 with polyA tail encoded

    Depositor

    Ralf Kuehn

    Article

    Chu et al BMC Biotechnol. 2016 Jan 16;16(1):4. doi

    Insert

    Cas9
    

    Use

    Tags

    Flag, NLS, and polyA tail

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Oct. 31, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAUC40

    Plasmid

    #71298

    Purpose

    Suicide Vector, Gateway attR-Chloramphenicol resistance cassette cloned in pKNG101

    Depositor

    Susanne von Bodman

    Article

    Carlier et al Mol Microbiol. 2009 Nov;74(4):903-13. do

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. p5M-PR-A

    Plasmid

    #89124

    Purpose

    mammalian expression of full-length human progesterone receptor-A in pCMV5 with 3 restriction sites deleted in polylinker

    Depositor

    Elizabeth Wilson

    Article

    Su et al J Biol Chem. 2012 Oct 5;287(41):34809-24

    Insert

    human progesterone receptor-A (PGR Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28b-His tagged nuclear Pif1

    Plasmid

    #65047

    Purpose

    expression of the nuclear form of Pif1p (Pif1p-delta40), Nterminal His tag

    Depositor

    Jean-Baptiste Boulé, Virgina Zakian

    Article

    Boule et al Nature. 2005 Nov 3;438(7064):57-61. Epub

    Insert

    Yeast Pif1, nuclear form, N-terminal 6x His-tag (PIF1 Budding Yeast)
    

    Use

    Tags

    histag

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Feb. 21, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCfB2312 (TEF1p-Cas9-CYC1t_kanMX)

    Plasmid

    #83946

    Purpose

    Cas9-carrying vector with kanMX marker

    Depositor

    Irina Borodina

    Article

    Stovicek et al Metab Eng Commun. 2015 Mar 20;2:13-22. d

    Insert

    Cas9
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Oct. 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. MmSphk1

    Plasmid

    #118599

    Purpose

    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate in mice

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Mus musculus sphingosine kinase 1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, 10

    Available since

    April 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. HsSPHK1

    Plasmid

    #118091

    Purpose

    Expresses sphingosine kinase 1, which catalyzes the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P)

    Depositor

    Kevin Lynch

    Article

    Kharel et al PLoS One. 2018 Apr 19;13(4):e0192179. do

    Insert

    Homo sapiens Sphingosine Kinase 1
    

    Use

    Tags

    FLAG

    Expression

    Yeast

    Mutation

    Promoter

    GAL1, 10

    Available since

    Aug. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. 12URA-B-ScTop2∆CTD

    Plasmid

    #214924

    Purpose

    Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

    Depositor

    James Berger

    Article

    Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

    Insert

    topoisomerase II
    

    Use

    Tags

    6x His-TEV

    Expression

    Yeast

    Mutation

    Deleted amino acids 1178-1428

    Promoter

    Gal 1/10

    Available since

    March 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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22. pBJ1806 pRS413-COF1RFP

    Plasmid

    #37101

    Depositor

    John Cooper

    Article

    Lin et al J Cell Sci. 2010 Apr 15;123(Pt 8):1329-4

    Insert

    COF1-RFP
    

    Use

    Tags

    RFP

    Expression

    Yeast

    Mutation

    Single RFP internal insertion at Cof147T48D with …

    Promoter

    Available since

    July 13, 2012

    Availability

    Academic Institutions and Nonprofits only

23. LV-TRE-WT mouse MyoD-T2A-dsRedExpress2

    Plasmid

    #60624

    Purpose

    Expresses WT mouse MyoD and dsRedExpress2 in response to doxycycline

    Depositor

    Charles Gersbach

    Article

    Kabadi et al ACS Synth Biol. 2014 Dec 10.

    Insert

    WT mouse MyoD (Myod1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    TRE

    Available since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pEF6 VPS52-V5-6His

    Plasmid

    #175096

    Purpose

    V5 tagged VPS52 is expressed in mammalian cells

    Depositor

    Juan Bonifacino

    Article

    Perez-Victoria et al Mol Biol Cell. 2008 Jun;19(6):2350-62. d

    Insert

    VPS52 (VPS52 Human)
    

    Use

    Tags

    V5

    Expression

    Mammalian

    Mutation

    N666D- please see depositor comment

    Promoter

    Available since

    Dec. 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. AB.pCCL.sin.cPPT.GFP.miR-126-3p.sensor.PGK.dNGFR.WPRE

    Plasmid

    #85908

    Purpose

    Sensor Plasmid

    Depositor

    Brian Brown

    Article

    Mullokandov et al Nat Methods. 2012 Jul 1;9(8):840-6. doi:

    Insert

    miR-126-3p Sensor (MIR126 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRG706_lexO-SbfI_LexA-TF_LEU2MX

    Plasmid

    #154818

    Purpose

    Inducible expression of SbfI endonuclease in S. cerevisiae

    Depositor

    Lorraine Symington

    Article

    Gnugge et al Nucleic Acids Res. 2020 Oct 14. pii: 592

    Inserts

    SbfI endonuclease
    LexA-ER-B112
    

    Use

    Tags

    FLAG and NLS

    Expression

    Yeast

    Mutation

    Promoter

    ACT1 and lexO_4-CYC1_core

    Available since

    Oct. 26, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pcDNA3-JNKAR1

    Plasmid

    #61625

    Purpose

    FRET-based biosensor for monitoring JNK activity

    Depositor

    Jin Zhang

    Article

    Fosbrink et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    JNKAR1
    

    Use

    Tags

    Citrine and ECFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pFA6a-GST-kanMX6

    Plasmid

    #39293

    Depositor

    John Pringle, Jurg Bahler

    Article

    Bahler et al Yeast. 1998 Jul;14(10):943-51.

    Insert

    KanR
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Aug. 20, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pDSAR-Cre-F2A-I-SceI

    Plasmid

    #62302

    Purpose

    Anopheles docking site transgenesis plasmid expressing Cre recombinase and I-SceI nuclease, vasa promoter, DsRed marker

    Depositor

    Eric Marois

    Article

    Volohonsky et al G3 (Bethesda). 2015 Apr 13. pii: g3.115.

    Insert

    Cre recombinase, I-SceI nuclease
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    vasa

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pFA6a-GST-His3MX6

    Plasmid

    #41604

    Depositor

    John Pringle

    Article

    Longtine et al Yeast. 1998 Jul;14(10):953-61.

    Insert

    His3
    

    Use

    Yeast genomic targeting

    Tags

    A. gossypii translation elongation factor 1a gene…

    Expression

    Mutation

    Promoter

    Available since

    Dec. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge