We narrowed to 167,150 results for: Gene

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pTK_BsmBI_LacZ_Citrine_Nanotag_24

Plasmid

#130522

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_70

Plasmid

#130532

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cerulean_Nanotag_75

Plasmid

#130534

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_9

Plasmid

#130516

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_BsmBI_LacZ_Citrine_Nanotag_5

Plasmid

#130515

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Oct. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Cherry_Nanotag_109

Plasmid

#130548

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 26, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_19_neg_Control

Plasmid

#130561

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_Citrine_Nanotag_32_neg_Control

Plasmid

#130563

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_BsmBI_LacZ_Cerulean_Nanotag_66

Plasmid

#130531

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_BsmBI_LacZ_Citrine_Nanotag_58

Plasmid

#130527

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_BsmBI_LacZ_Citrine_Nanotag_35

Plasmid

#130524

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_BsmBI_LacZ_Citrine_Nanotag_18

Plasmid

#130519

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pTK_BsmBI_LacZ_Citrine_Nanotag_17

Plasmid

#130518

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_11

Plasmid

#130517

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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lenti SYN-SVI-dCas9-VPR

Plasmid

#164575

Purpose

Expresses an intron-containing dCas9-VPR fusion driven by human SYN promoter

Depositor

Jeremy Day

Article

Carullo et al eNeuro. 2021 Jul 26. pii: ENEURO.0188-21

Insert

FLAG-dCas9-VPR containing an SV40 intron

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_Psyn-sgRNAmreB

Plasmid

#149653

Purpose

all-in-one CRISPRi vector for targeting B. burgdorferi mreB

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Insert

dCas9, lacI, sgRNAmreB

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30, Psyn

Available Since

Dec. 1, 2020

Availability

Academic Institutions and Nonprofits only

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pCGN-ELK-1 R mut

Plasmid

#27159

Purpose

For mammalian expression of human Elk-1 with an HA tag and a mutation in the R repression domain

Depositor

Ron Prywes

Article

Lee et al J Biol Chem. 2010 Jul 16. 285(29):22036-

Insert

ELK1 (ELK1 Human)

Tags

HA

Expression

Mammalian

Mutation

R box mutant: K249A/V250A/E251A

Available Since

Feb. 28, 2011

Availability

Academic Institutions and Nonprofits only
BC-A1-014

Plasmid

#78702

Purpose

A weak promoter drives the expression of a hypersensitive luxR mutant, which regulates expression of GFP under the control of a strong RBS.

Depositor

Brian Chow

Article

Magaraci et al ACS Synth Biol. 2016 Apr 25.

Insert

BBa_J23109-BBa_B0034-luxR(G2F)-Terminator-pLux-BBa_B0034-GFP-Terminator

Use

Synthetic Biology

Available Since

Jan. 31, 2017

Availability

Academic Institutions and Nonprofits only

Enlarge
pAc5.1B-lambdaN-HA-DmTral

Plasmid

#21721

Depositor

Elisa Izaurralde

Article

Eulalio et al Mol Cell Biol. 2007 Jun . 27(11):3970-81

Insert

Trailer hitch (tral Fly)

Use

Drosophila expression

Tags

lambdaN-HA

Expression

Mammalian

Available Since

Aug. 5, 2009

Availability

Academic Institutions and Nonprofits only

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pEE385

Plasmid

#149289

Purpose

T-DNA encoding TRV2 with Truncated FT augmented gRNA targeting NbAG

Depositor

Daniel Voytas

Article

Ellison et al Nat Plants. 2020 Jun 1. pii: 10.1038/s41

Insert

p35S:TRV2_NbAGsgRNA2_TrunFT:tNos

Expression

Plant

Promoter

35S

Available Since

July 20, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-ZKSCAN1 548-1417 (No intron)

Plasmid

#69904

Purpose

Expresses a miniature version of the ZKSCAN1 circular RNA in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1 (ZKSCAN1 Human)

Expression

Mammalian

Mutation

F169L in ZKSCAN1

Promoter

CMV

Available Since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-PGK-SNAP-MDMUT-SOX2

Plasmid

#115688

Purpose

Constitutive expression of a SOX2 protein tagged with SNAP-tag and a non-functional mitotic degron (MDMUT) for control

Depositor

David Suter

Article

Deluz et al Genes Dev. 2016 Nov 15;30(22):2538-2550.

Insert

SNAP-MDMUT-SOX2

Use

Lentiviral

Available Since

Sept. 18, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA-Evf2

Plasmid

#99478

Purpose

Mammalian expression of the Evf2 long non-coding RNA

Depositor

Jhumku Kohtz

Article

Feng et al Genes Dev. 2006 Jun 1;20(11):1470-84. Ep

Insert

Evf2

Expression

Mammalian

Available Since

Nov. 8, 2017

Availability

Academic Institutions and Nonprofits only

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pBbdCas9S_arr2

Plasmid

#149639

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Nov. 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBRIT Pax7 N281

Plasmid

#17524

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 281 amino acids

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
HOXC5-pCT

Plasmid

#20999

Depositor

Corey Largman

Article

Lawrence et al Cell Growth Differ. 1993 Aug . 4(8):665-

Insert

HOXC5 (HOXC5 Human)

Expression

Mammalian

Available Since

Aug. 14, 2009

Availability

Academic Institutions and Nonprofits only
pU6Rosa-CAG-Gal4-Rad18UBD

Plasmid

#119012

Purpose

Mammalian expression of Rosa26 sgRNA, fusion protein of yeast Gal4 with human Rad18 Ubiquitin binding domain and BFP expression

Depositor

Ralf Kuehn

Article

Bashir et al BMC Biotechnol. 2020 Oct 23;20(1):57. do

Insert

Gal4 (GAL4 Budding Yeast)

Expression

Mammalian

Promoter

CAG

Available Since

Oct. 28, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbR-HIS-CBM-CohI

Plasmid

#58709

Purpose

Probing molecule to perform single molecule force spectroscopy experiments

Depositor

Hermann Gaub

Article

Otten et al Nat Methods. 2014 Sep 7. doi: 10.1038/nm

Insert

CBM-CohI

Tags

HIS, HRV 3C, and ybbR

Expression

Bacterial

Mutation

changed Alanine 2 to Cysteine in CBM domain

Promoter

T7

Available Since

Aug. 25, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTRdeltaGFPmirGE-DroshaT1.5.5

Plasmid

#61084

Purpose

pENTR plasmid containing a mutated GFP followed by 3 miRNA haipins: recommended as backbone.

Depositor

Patrick Salmon

Article

Myburgh et al Mol Ther Nucleic Acids. 2014 Oct 28;3:e2

Insert

deltaGFP

Use

RNAi

Available Since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrichoGate-10

Plasmid

#160710

Purpose

For C-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic mCherry

Use

Synthetic Biology

Available Since

March 2, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrichoGate-8

Plasmid

#160708

Purpose

For N-terminal tags with Trichoderma optimised Synthetic eGFP bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic eGFP

Use

Synthetic Biology

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pTrichoGate-7

Plasmid

#160707

Purpose

For N-terminal tags with Trichoderma optimised Synthetic mCherry bordered by BsaI restriction sites

Depositor

Artemio Mendoza

Article

Nogueira-Lopez et al Front Microbiol. 2019 Dec 10;10:2794. do

Insert

Trichoderma optimised Synthetic mCherry

Use

Synthetic Biology

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
LacD_WT_pET21a

Plasmid

#29785

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LacD_L165E_pET21a

Plasmid

#29786

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Mutation

L165E

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LacD_L275S_pET21a

Plasmid

#29787

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Mutation

L275S

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
LacD_L165E/L275S_pET21a

Plasmid

#29788

Depositor

SeWon Suh

Article

Lee et al FEBS Lett. 2011 Jan 21. 585(2):307-12.

Insert

Tagatose 1,6-diphosphate aldolase

Tags

His

Expression

Bacterial

Mutation

L165E and L275S

Available Since

July 7, 2011

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-Fos Y345A

Plasmid

#8970

Depositor

John Blenis

Article

Murphy et al Nat Cell Biol. 2002 Aug . 4(8):556-64.

Insert

c-Fos (Fos Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Y345A

Available Since

Sept. 2, 2005

Availability

Academic Institutions and Nonprofits only
pmyc-GFP-TNRC6C-(928-1020)-NES-mut

Plasmid

#42027

Depositor

Kumiko Ui-Tei

Article

Nishi et al RNA. 2013 Jan;19(1):17-35. doi: 10.1261/

Insert

TNRC6C (TNRC6C Human)

Tags

EGFP and Myc

Expression

Mammalian

Mutation

contain aa928-1020, L943A, L947A, M950A, F952A

Promoter

CMV

Available Since

March 14, 2013

Availability

Academic Institutions and Nonprofits only

Enlarge
pENTR-L3-Cas9-L2

Plasmid

#141038

Purpose

Mutisite Gateway mediated vector construction

Depositor

Hideyuki Okano

Article

Yoshimatsu et al PLoS One. 2019 Aug 27;14(8):e0221164. do

Insert

Cas9

Expression

Mammalian

Available Since

June 25, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
TFF1_pCSdest

Plasmid

#53908

Depositor

Roger Reeves

Article

Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

Insert

TFF1 (TFF1 Human)

Expression

Mammalian

Promoter

SP6 and CMV

Available Since

March 27, 2018

Availability

Industry, Academic Institutions, and Nonprofits
px330BRG1 gRNA

Plasmid

#165585

Purpose

Insertion of BRG1 degron

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

BRG1 (Smarca4 Mouse)

Expression

Mammalian

Mutation

NA

Available Since

March 10, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFlox c-SPT5_EYFP_FKBPF36V_Neo

Plasmid

#163872

Purpose

Acute Depletion of SPT5-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Spt5 (Supt5 Mouse)

Expression

Mammalian

Available Since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
TAL3323

Plasmid

#42786

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-nr5a2-right (nr5a2 Zebrafish)

Use

t7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

March 19, 2013

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 N391

Plasmid

#17525

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

N-terminal 391 amino acids

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
pBbdCas9G_arr2

Plasmid

#149571

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
pBbdCas9G(RBSmut)_arr2

Plasmid

#149578

Purpose

parental, all-in-one CRISPRi vector for B. burgdorferi, parental for gRNA cloning

Depositor

Christine Jacobs-Wagner

Article

Takacs et al Appl Environ Microbiol. 2020 Nov 30. pii

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

PflaB, PpQE30

Available Since

Oct. 26, 2020

Availability

Academic Institutions and Nonprofits only

Enlarge
Nanog-t2A-mCherry

Plasmid

#127541

Purpose

Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

Codes for the last 110 a.a of Nanog

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFlox_N-BRG1_BSD_FKBPF36V_EYFP

Plasmid

#163874

Purpose

Acute Depletion of BRG1-EYFP

Depositor

Cornelis Murre

Article

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Insert

Brg1 (Smarca4 Mouse)

Expression

Mammalian

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pG3GB411

Plasmid

#134748

Purpose

pGreen3 CRISPR/Cas9

Depositor

Qi-Jun Chen

Article

Zhang et al Plant Physiol. 2019 Sep 26. pii: pp.19.0

Insert

CRISPR/Cas9

Use

CRISPR

Promoter

gRNA- OsU3, zCas9-Ubi1

Available Since

Dec. 12, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
Beta lactamase S68TAG

Plasmid

#68305

Purpose

Beta lactamase S68TAG gene

Depositor

Jesse Rinehart

Article

Pirman et al Nat Commun. 2015 Sep 9;6:8130. doi: 10.1

Insert

S68TAG beta lactamase

Use

Synthetic Biology

Expression

Bacterial

Available Since

Sept. 10, 2015

Availability

Academic Institutions and Nonprofits only

Enlarge

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We narrowed to 167,150 results for: Gene