We narrowed to 125,794 results for: CIL

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opCas12a-T8N (pZZ34, TAT-6MycNLS-opCas12a-2SV40NLS-sfGFP)

Plasmid

#199605

Purpose

Expression of cell permeable opAsCas12a in E. Coli.

Depositor

Junwei Shi

Article

Zhang et al Nat Biotechnol. 2023 Apr 24. doi: 10.103

Insert

opCas12a-T8N

Use

CRISPR

Tags

6xHis, Twin-strep, SUMO

Expression

Bacterial

Available Since

April 28, 2023

Availability

Academic Institutions and Nonprofits only

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SQT1665

Plasmid

#78744

Purpose

Human expression plasmid for LbCpf1-NLS-3xHA

Depositor

Keith Joung

Article

Kleinstiver et al Nat Biotechnol. 2016 Jun 27. doi: 10.103

Insert

mammalian codon-optimized Lachnospiraceae bacterium ND2006 Cpf1-NLS-3xHA

Use

CRISPR

Tags

NLS-3xHA

Expression

Mammalian

Promoter

CAG

Available Since

June 16, 2016

Availability

Academic Institutions and Nonprofits only

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pGL2-AN

Plasmid

#20950

Depositor

William Kaelin

Article

Neuman et al Mol Cell Biol. 1994 Oct . 14(10):6607-15

Insert

E2F-1 promoter, genomic fragment (E2F1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

wt human E2F-1 promoter sequence

Available Since

Aug. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) ZKSCAN1 MCS-WT Split GFP + Sense IRES

Plasmid

#69909

Purpose

Expresses a GFP circular RNA containing the sense EMCV IRES in mammalian cells

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

ZKSCAN1, GFP (ZKSCAN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLemir9-124

Plasmid

#31779

Depositor

Jerry Crabtree

Article

Yoo et al Nature. 2011 Jul 13. doi: 10.1038/nature

Insert

miR-9/9* and miR-124 (Mir124a-2, Mir9-3 Mouse)

Use

Lentiviral

Available Since

Sept. 22, 2011

Availability

Academic Institutions and Nonprofits only

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CARPID BASU-dCasRx

Plasmid

#153209

Purpose

express CARPID BASU-dCasRx fusion protein in mammalian cells

Depositor

Jian Yan, Liang Zhang

Article

Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

Insert

BASU-dCasRx

Use

CRISPR and Lentiviral

Expression

Mammalian

Mutation

R239A/H244A/R858A/H863A in RfxCas13d

Promoter

EF-1a

Available Since

June 29, 2020

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-Nanoluc-ccdB

Plasmid

#87078

Purpose

Lentiviral Gateway-compatible expression vector backbone with an N-terminal Nanoluc luciferase

Depositor

Mikko Taipale

Type

Empty backbone

Use

Lentiviral

Tags

Nanoluc luciferase

Expression

Mammalian

Available Since

March 7, 2017

Availability

Academic Institutions and Nonprofits only

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mVenus-pBAD

Plasmid

#54845

Purpose

Localization: Protein Expression Vector , Excitation: 515, Emission: 528

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Nagai et al Nat Biotechnol. 2002 Jan;20(1):87-90.

Type

Empty backbone

Tags

6xHis and mVenus

Expression

Bacterial

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pLVX-EF1alpha-SARS-CoV-2-nsp15-2xStrep-IRES-Puro

Plasmid

#141381

Purpose

Lentiviral expression of SARS-CoV-2 protein; transient expression and generate lentivirus

Depositor

Nevan Krogan

Article

Gordon et al Nature. 2020 Jul;583(7816):459-468. doi:

Insert

SARS-CoV-2-nsp15 (ORF1ab SARS-CoV-2)

Use

Lentiviral

Tags

2xStrep

Expression

Mammalian

Mutation

human codon optimized

Promoter

EF1alpha

Available Since

April 9, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.2/DEST/hTfR-HA

Plasmid

#69610

Purpose

human transferrin receptor with an HA tag fused at the C-terminus

Depositor

Robin Shaw

Article

Smyth et al Circ Res. 2012 Mar 30;110(7):978-89. Epu

Insert

human transferrin receptor (TFRC Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 1, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-AsCas12f1

Plasmid

#171614

Purpose

AsCas12f1-based Human cell genome editing

Depositor

Quanjiang Ji

Article

Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

Insert

AsCas12f1 and sgRNA_v1

Expression

Mammalian

Available Since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

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pLentiCRISPR-mCherry

Plasmid

#75161

Purpose

Derived from LentiCRISPR v1 but expresses mCherry instead of puromycin resistance. 3rd generation.

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

mCherry

Expression

Mammalian

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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pTT5-hSDC1

Plasmid

#52326

Purpose

expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture.

Depositor

Gordon Laurie

Article

Zhang et al J Biol Chem. 2013 Apr 26;288(17):12090-1

Insert

SDC1 (SDC1 Human)

Expression

Mammalian

Mutation

R117Q mutation (R95Q according to numbering in Fi…

Promoter

CMV

Available Since

Dec. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pJRH-1179 U6-reci Gag-Cas9 v2

Plasmid

#201914

Purpose

Cas9-EDV production plasmid. Expresses the v2 Gag-Cas9 polypeptide (CAG promoter) and sgRNA (human U6 promoter. BsmBI cutsites for spacer insertion)

Depositor

Jennifer Doudna

Article

Hamilton et al Nat Biotechnol. 2024 Jan 11. doi: 10.103

Insert

Gag-Cas9 v2

Expression

Mammalian

Promoter

CAG

Available Since

Aug. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pLH-stsgRNA3.1

Plasmid

#64118

Purpose

Vector for expression of St1 sgRNA3.1 in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

St1 sgRNA3.1

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

April 24, 2015

Availability

Academic Institutions and Nonprofits only

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mTFP1-pBAD

Plasmid

#54553

Purpose

Localization: Protein Expression Vector , Excitation: 462, Emission: 492

Depositor

Robert Campbell, Michael Davidson

Article

Ai et al Biochem J. 2006 Dec 15. 400(3):531-40.

Type

Empty backbone

Tags

6xHis and mTFP1

Expression

Bacterial

Available Since

July 25, 2014

Availability

Academic Institutions and Nonprofits only

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pGL3b-8XGliBS:EGFP

Plasmid

#84602

Purpose

Gli protein-dependent EGFP reporter for analysis of Hedgehog signaling

Depositor

James Chen

Article

Hyman et al Proc Natl Acad Sci U S A. 2009 Aug 18;10

Insert

eGFP

Expression

Mammalian

Promoter

8X Gli-binding site

Available Since

Oct. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pHAGE-TO-nmdCas9-3XGFP

Plasmid

#64109

Purpose

Expression of Nm dCas9-3XGFP in mammalian cells

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2015 Mar 10;11

Insert

Nm dCas9

Use

CRISPR and Lentiviral

Tags

3XsfGFP

Expression

Mammalian

Promoter

CMV-TO

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DIO-Sun1GFP-WPRE-pA

Plasmid

#160141

Purpose

Cre- dependent expression of Sun1GFP for nuclei isolation

Depositor

Andreas Pfenning

Article

Lawler et al J Neurosci. 2020 Nov 10. pii: JNEUROSCI.

Insert

Sun1-GFP (Sun1 Mouse)

Use

AAV

Tags

GFP

Expression

Mammalian

Mutation

N-truncated: removed amino acids 1-207, retained …

Promoter

Ef1a

Available Since

March 6, 2021

Availability

Academic Institutions and Nonprofits only

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pJ3 SHP1 WT

Plasmid

#8572

Depositor

Ben Neel

Insert

SHP1 (PTPN6 Human)

Expression

Mammalian

Available Since

June 17, 2005

Availability

Academic Institutions and Nonprofits only