We narrowed to 7,702 results for: 104
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Plasmid#104397PurposeThe designed sgRNA cloned into this plasmid directs the specific DNA cleavage exerted by Cas9 nuclease in a region of exon 5 of the human TK2 gene. The plasmid also includes the Cas9 nuclease and CD4.DepositorInsertTK2 (TK2 Human)
UseTagsExpressionMammalianMutationPromoterU6Available sinceApril 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4L WW domain #1
Plasmid#104204PurposeBacterial expression of WW domain #1 from NEDD4LDepositorInsertNEDD4L WW domain #1 (NEDD4L Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSR26-2
Plasmid#104335PurposeExpresses ompR from a TetR reuglated promoter under inducible ATC control.DepositorInsertOmpR (ompR Escherichia coli str. K-12 substr. MG1655)
UseTagsExpressionBacterialMutationPromoterPLTetO1Available sinceApril 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
MBP-hnRNPA2_FL_P298L
Plasmid#104468Purposeexpress MBP-tagged full length hnRNPA2 P298LDepositorInserthnRNPA2 (HNRNPA2B1 Human)
UseTagsHis-MBPExpressionBacterialMutationP298LPromoterAvailable sinceJan. 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC-elav1.8kb-TALE1-VP64-P10
Plasmid#104609PurposeExpresses TALE1 under the control of a 1.8kb elav enhancer elementDepositorInsert1.8kb elav enhancer element (elav Fly)
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
mGarnet-RITA
Plasmid#104313Purposecodes for a fusion of fluorescent protein mGarnet and RITA (RBP-J Interacting and tubulin-associated), stains microtubular stucturs in live cellsDepositorInsertmGarnet-RITA
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceOct. 25, 2018AvailabilityAcademic Institutions and Nonprofits only -
JG674: CAG-human dLbCpf1(D832A)-NLS-3xHA-3xFLAG-DmrA(X1)
Plasmid#104568PurposeMammalian expression vector for catalytically inactive Cpf1 from Lachnospiraceae bacterium (dLbCpf1) fused to one DmrA domainDepositorInsertdLbCpf1(D832A)-DmrA(x1)
UseTags3x FLAG, 3x HA, and NLSExpressionMammalianMutationD832APromoterCAGAvailable sinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 NEDD4L WW domain #4
Plasmid#104207PurposeBacterial expression of WW domain #4 from NEDD4LDepositorInsertNEDD4L WW domain #4 (NEDD4L Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
LZF70: Cre-off hSyn-QuasAr3-dark Citrine (FAS)
Plasmid#104117PurposesynOptopatch constructDepositorInsertQuasAr3-dark Citrine
UseLentiviralTagsExpressionMammalianMutationPromoterhuman synapsin IAvailable sinceOct. 12, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28b – E. coli RecE residues 564-868
Plasmid#104534PurposeExpresses E. coli RecE residues 564-868DepositorInsertRecE residues 564-866 (C-terminal nuclease domain)
UseTags6X-His tag followed by thrombin siteExpressionBacterialMutationPromoterT7Available sinceDec. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
GST-AR-334-566
Plasmid#104198PurposeExpresses human AR-334-566 in pGEX-4T-1 bacterial expression vector as fusion protein with GSTDepositorInserthuman androgen receptor aa 334-566 (AR Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSF4 TetCMV intron 4xGCN4 Renilla FKBP STOP 24xMS2v5 SV40 CTE polyA
Plasmid#104984PurposeExpresses ST-Renilla reporter in mammalian cellsDepositorInsertRenilla luciferase
UseTags24xMS2v5 stem loops in 3'UTR, 4xGCN4 repeats…ExpressionBacterial and MammalianMutationPromoterTetCMVAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC43
Plasmid#104817PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from Gmubi promoter from rolD promoterDepositorInsertGlyma.04g057400
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSC44
Plasmid#104818PurposeCRISPR/Cas9 1xplex gRNA targeting Glyma.04g057400 (Dcl3a-1). Also expresses Cas9 from AtUBQ10 promoter.DepositorInsertGlyma.04g057400
UseCRISPRTagsExpressionPlantMutationPromoterAvailable sinceFeb. 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 C20orf67 WW domain
Plasmid#104273PurposeBacterial expression of WW domain from C20orf67DepositorInsertC20orf67 WW domain (PCIF1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
JC021
Plasmid#104944PurposeEstrogen inducing G-CSFDepositorInsertG-CSF
UseTagsExpressionMutationPromoterAvailable sinceJune 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pENTR-HLA-B15-His
Plasmid#104837PurposeHLA-B150101 cloned as a His tagDepositorInsertmajor histocompatibility complex, class I, B (HLA-B Human)
UseEntry vector for gateway systemTagshisExpressionMutation2 differences compared to original B15: L2->R …PromoterAvailable sinceDec. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJC-mhc2.4kb-TALE3-VP64-P10
Plasmid#104611PurposeExpresses TALE3 under the control of a 2.4kb mhc enhancer elementDepositorInsert2.4kb mhc enhancer element (Mhc Fly)
UseTALENTagsExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103 IQGAP1 WW domain
Plasmid#104279PurposeBacterial expression of WW domain from IQGAP1DepositorInsertIQGAP1 WW domain (IQGAP1 Human)
UseTagsGSTExpressionBacterialMutationcodon optimized for expression in bacteriaPromotertacAvailable sinceAug. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
PB-eDIO-ChR2-EYFP
Plasmid#104387PurposePiggyBac plasmid encoding channelrhodopsin 2 fused to EYFP in an inverse orientation flanked by loxP and lox2722 sites under the control of the EF1a promotor.DepositorInsertChannelrhodopsin 2
UseCre/Lox; PiggybacTagsEYFPExpressionMammalianMutationE134RPromoterAvailable sinceFeb. 15, 2018AvailabilityAcademic Institutions and Nonprofits only