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Plasmid#236228PurposeAAV expression of a marker for the endoplasmic reticulum, Sec61b, fused to mEmerald.DepositorInsertmEmerald-Sec61b (SEC61B Synthetic, Human)
UseAAVTagsmEmeraldExpressionMutationPromoterhuman Synapsin 1Available sinceMay 1, 2025AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT-TO-NOD2-EGFP
Plasmid#131207Purposemammalian expression, tet RDepositorInsertNOD2 (NOD2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterpCMVAvailable sinceSept. 5, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCMV-monSTIM1
Plasmid#161795PurposeMammalian expression plasmid of monSTIM1 (ultra-photosensitive optogenetically-activated STIM1 protein)DepositorInsertMonSTIM1 (STIM1 Mustard Weed, Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceAug. 10, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-Synaptojanin 1-170
Plasmid#22292DepositorInsertSynaptojanin 1_170 (SYNJ1 Human)
UseTagsFlagExpressionBacterial and MammalianMutationK334R compared to Genbank ID NM_003895PromoterAvailable sinceOct. 9, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1-GFP (A317-318; A341)
Plasmid#204198PurposeMammalian expression of SREBP-1 (isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTags3xHA, eGFP and T7x2ExpressionMammalianMutationGly to Ala 317,318,341PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pLenti-gfaABC1D-monSTIM1
Plasmid#161797PurposeLentiviral vector expressing monSTIM1 in astrocytes.DepositorInsertEGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromotergfaABC1DAvailable sinceAug. 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CamKIIa-monSTIM1
Plasmid#161796PurposeLentiviral vector expressing monSTIM1 in neurons.DepositorInsertEGFP-CRY2PHR(mon)-STIM1 (STIM1 Human)
UseLentiviralTagsEGFPExpressionMutationPromoterCamKIIaAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3- SREBP-1 (A317-318; L340)
Plasmid#204195PurposeMammalian expression of SREBP-1 (Isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationGly to Ala 317,318; Gly to Leu 340PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3- SREBP-1 (A317-318;A341; A1171)
Plasmid#204196PurposeMammalian expression of SREBP-1 (isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationGly to Ala 317,318,341,1171PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-cka
Plasmid#195067PurposeExpresses FLAG-tagged Drosophila cka proteinDepositorInsertConnector of kinase to AP-1 (Cka Fly)
UseTagsExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
CHMP2B_Halo_C_allele
Plasmid#178153PurposeDonor vector for endogenous tagging of human CHMP2B at the C-terminus with halotagInsertHalotag (CHMP2B Human)
UseDonor vectorTagsExpressionMutationPromoterAvailable sinceDec. 2, 2021AvailabilityAcademic Institutions and Nonprofits only -
anxA2-GFP
Plasmid#107196PurposeExpresses human annexin A2-GFP fusion protein for live cell imagingDepositorInsertannexin A2 (ANXA2 Human)
UseTagsGreen Fluorescent ProteinExpressionMammalianMutationAla residue 65 in human sequence replaced by Glu …PromoterCMVAvailable sinceJune 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
6-His-MBP-TEV-FnCpf1
Plasmid#90094PurposeBacterial expression plasmid for protein purificationDepositorInsertFnCpf1 (humanized)
UseTags3xHA, 6xHis, MBP, Nucleoplasmin NLS, and TEV site…ExpressionBacterialMutationPromoterT7Available sinceJune 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+FLAG-KrasnatG12D
Plasmid#206842PurposeTo express mouse Kras encoded by native mouse codons and with a G12D mutation with an N-terminal FLAG tag.DepositorInsertKras (Kras Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Glycine 12 to Aspartic AcidPromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 _3xHA-Ago2
Plasmid#73538PurposeInducible lentiviral expression of Ago2DepositorInsertAgo2 (Ago2 Mouse)
UseLentiviralTags3xHAExpressionMammalianMutationPromoterTRE promoter, Tet ONAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET22-His-TEV-DOT1L
Plasmid#89610PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L residues 1-416DepositorInsertDot1L (DOT1L Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceAug. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1-GFP (A317-318; A341; A1171)
Plasmid#204199PurposeMammalian expression of SREBP-1 (isoform 1)DepositorInsertSREBP-1 (SREBF1 Human)
UseTags3xHA, eGFP and T7x2ExpressionMammalianMutationGly to Ala 317,318,341,1171PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+FLAG-KrasnatQ61R
Plasmid#206843PurposeTo express mouse Kras encoded by native mouse codons and with a Q61R mutation with an N-terminal FLAG tag.DepositorInsertKras (Kras Mouse)
UseTagsFLAGExpressionMammalianMutationchanged Glutamine 61 to ArgininePromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pET22-His-TEV-DOT1L-MM
Plasmid#89611PurposeBacterial expression vector for an N-terminally His-tagged (TEV-cleavable) version of human Dot1L hypermorphic MM mutant residues 1-416DepositorInsertDot1L VVEL293MM Mutant (DOT1L Human)
UseTagsHis-TEVExpressionBacterialMutationPromoterT7Available sinceApril 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 _3xHA-Ago2_RNAmut
Plasmid#73539PurposeInducible lentiviral expression of Ago2_RNAmutDepositorInsertAgo2 (Ago2 Mouse)
UseLentiviralTags3xHAExpressionMammalianMutationSmall RNA binding mutant PAZ domain Y311A and F31…PromoterTRE promoter, Tet ONAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCW57.1 _3xHA-Ago2_CATmut
Plasmid#73540PurposeInducible lentiviral expression of Ago2_CATmutDepositorInsertAgo2 (Ago2 Mouse)
UseLentiviralTags3xHAExpressionMammalianMutationCatalytic mutant PIWI domain D670APromoterTRE promoter, Tet ONAvailable sinceFeb. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
LADL Bridge + Target Zfp462-Klf4SE
Plasmid#127668PurposeEncodes for CRY2-HA and mCherry proteins expressed from the EF1a promoter; 2 gRNAs targeting the Zfp462 promoter and 2 gRNAs targeting the Klf4 SE expressed from their individual hU6 promotersDepositorInsertCRY2-HA-2A-mCherry and gRNAs 129, 135, 115 and 117
UseCRISPRTagsHAExpressionMammalianMutationPromoterEF1a and hU6Available sinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
pEQ111m
Plasmid#40190DepositorInsertEcoRI endonuclease & EcoRI methylase
UseTagsExpressionBacterialMutationchanged Glutamic Acid 111 to GlutaminePromoterLambda PLAvailable sinceNov. 19, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBAD-I-SceI
Plasmid#60960PurposePlasmid that allows an arabinose-inducible expression of the I-SceI homing endonuclease - for the en passant (two-step Red-mediated) mutagenesisDepositorInsertI-SceI restriction enzyme
UseTagsExpressionMutationPromoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRM049 His-TEV-Cas9 triNLS 2C
Plasmid#196245PurposeExpression of NLS-rich Cas9 (2 Cys residues, mammalian codon optimized)DepositorInsertCas9
UseCRISPRTagsHA, His6 (N terminal on backbone), NLS, Nucleopla…ExpressionBacterialMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-SREBP-1 S1P and S2P mutations (U124AFG200-5)
Plasmid#210767PurposeMammalian expression of SREBP-1 (isoform 1) with mutations in residues reported to be critical for S1P and S2P cleavage.DepositorInsertSREBP-1 (SREBF1 Human)
UseTagsE-tag and T7x2ExpressionMammalianMutationDRSR 514-517 to AS; 557 R to A; multiple silent m…PromoterAvailable sinceApril 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Mts
Plasmid#195065PurposeExpresses FLAG-tagged Drosophila mts proteinDepositorInsertmicrotubule star (mts Fly)
UseTags3xFLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1+FLAG-KrascomG12D
Plasmid#206844PurposeTo express mouse Kras encoded by common mouse codons (to increase expression) and with a G12D mutation with an N-terminal FLAG tag.DepositorInsertKras (Kras Mouse)
UseTagsFLAGExpressionMammalianMutationCodons altered to most optimum based on mouse cod…PromoterAvailable sinceOct. 3, 2023AvailabilityAcademic Institutions and Nonprofits only