Addgene

We narrowed to 622 results for: golgi

Showing: 541 - 570 of 622 results

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mPapaya-MannII-N-10

Plasmid

#56347

Purpose

Localization: Mannosidase/Golgi, Excitation: 530, Emission: 541

Depositor

Michael Davidson

Article

Hoi et al Chem Biol. 2013 Oct 24;20(10):1296-304.

Insert

MannII

Use

Tags

mPapaya

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Academic Institutions and Nonprofits only

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mTFP1-SiT-N-15

Plasmid

#55508

Purpose

Localization: Golgi TGN, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

SiT (ST6GAL1 Human)

Use

Tags

mTFP1

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available since

Oct. 2, 2014

Availability

Academic Institutions and Nonprofits only
GALT-SEP-mRuby3

Plasmid

#200938

Purpose

trans-Golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

GalT (B4GALT1 Human)
SEP-mRuby3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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MGAT-SEP-mRuby3

Plasmid

#200937

Purpose

cis/medial golgi localized pH sensor using sensorsuperecliptic pHluorin (SEP) and mRuby3

Depositor

Nir Hacohen

Article

Liu et al Science. 2023 Aug 4;381(6657):508-514. d

Inserts

MGAT2 (MGAT2 Human)
SEP-mRuby3

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-GalT-IRES-Puromycin

Plasmid

#134862

Purpose

Expresses EGFP-tagged Golgi marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

beta 1,4-galactosyltransferase (B4GALT1 Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 83-398

Promoter

EF1a

Available since

Jan. 27, 2021

Availability

Academic Institutions and Nonprofits only

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pYFP-NGAT(A193T,N194Y)

Plasmid

#11388

Depositor

Joel Swanson

Article

Beemiller et al PLoS Biol. 2006 May 9. 4(6):e162.

Insert

golgi associated, gamma adaptin ear containing, ARF binding protein 1 (GGA1 Human)

Use

Tags

YFP

Expression

Mammalian

Mutation

Changed Alanine 193 to Threonine, and Asparagine …

Promoter

Available since

March 3, 2006

Availability

Academic Institutions and Nonprofits only

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mRuby2-MannII-N-10

Plasmid

#55903

Purpose

Localization: Mannosidase/Golgi, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MannII

Use

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Clover-MannII-N-10

Plasmid

#56302

Purpose

Localization: Mannosidase/Golgi, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

MannII

Use

Tags

Clover

Expression

Mammalian

Mutation

aa 1-112 of MannII

Promoter

CMV

Available since

Dec. 18, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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XZ362 EF1a-VEGFR2sp-HA-VEGFR2(Ecto+TM)-TS-tdMCP-export

Plasmid

#233381

Purpose

Optimized VEGF-sensing eLIDAR with VEGFR2 ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

VEGFR2(ecto+TM)-tdMCP

Use

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ363 EF1a-VEGFR1sp-3xFlag-VEGFR1(ecto+TM)-TS-ADAR2dd(QA)-export

Plasmid

#233382

Purpose

Optimized VEGF-sensing eLIDAR with VEGFR1 ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

VEGFR1(ecto+TM)-ADAR2dd

Use

Tags

FLAG

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ375 EF1a-IL2Rbsp-3xFLAG-IL2Rb(ECD+TM)-TS-ADAR2dd-export

Plasmid

#233386

Purpose

Optimized IL-2-sensing eLIDAR with IL-2 beta chain ecto+TM domains fused with ADAR2dd, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IL2Rb(ecto+TM)-ADAR2dd

Use

Tags

FLAG

Expression

Mammalian

Mutation

E488Q, T501A on ADAR2dd

Promoter

EF1a

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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XZ374 EF1a-IL2Rgsp-3xHA-IL2Rg(ECD+TM)-TS-tdMCP-export

Plasmid

#233389

Purpose

Optimized IL-2-sensing eLIDAR with IL-2 gamma chain ecto+TM domains fused with tdMCP, with Golgi traffic sequence and ER export sequence

Depositor

Xiaojing Gao

Article

Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

Insert

IL2Rg(ecto+TM)-tdMCP

Use

Tags

HA

Expression

Mammalian

Mutation

WT

Promoter

EF1a

Available since

April 1, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-ANP1-FAPoptim

Plasmid

#221120

Purpose

FAP-tagged marker for the cis-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

ANP1-FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pRS413-TEF1pr-SEC7-FAPoptim

Plasmid

#221124

Purpose

FAP-tagged marker for the trans-Golgi (FAP optimized for yeast expression)

Depositor

Allyson O’Donnell

Article

Oppenheimer et al Mol Biol Cell. 2024 Jul 1;35(7):mr5. doi

Insert

SEC7-FAPoptim

Use

Tags

Expression

Yeast

Mutation

Promoter

TEF1

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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SEC7-2EGFP-ter-NAT

Plasmid

#184764

Purpose

Marker for yeast late Golgi/early endosome . Integration of 2xGFP tag at SEC7 C terminus. Uses antibiotic resistance marker natMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SYS1-2EGFP-ter-URA3

Plasmid

#184771

Purpose

Marker for yeast early Golgi. Integration of 2xGFP tag at SYS1 C terminus. Uses auxotrophic marker URA3(Candida albicans).

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SYS1 (SYS1 Budding Yeast)

Use

Tags

2EGFP

Expression

Yeast

Mutation

Promoter

Available since

Feb. 6, 2023

Availability

Academic Institutions and Nonprofits only

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SEC7-2Katushka-ter-Hygromycin

Plasmid

#184772

Purpose

Red marker for yeast late Golgi/early endosome. Integration of 2xKatushka tag at SEC7 C terminus. Uses antibiotic resistance marker hphMX6.

Depositor

Zhiping Xie

Article

Li et al BMC Biol. 2020 Aug 28;18(1):107. doi: 10

Insert

SEC7 (SEC7 Budding Yeast)

Use

Tags

2Katushka

Expression

Yeast

Mutation

Promoter

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pME-mScarlet3-Giantin (JDW 1356)

Plasmid

#224502

Purpose

Gateway compatible middle entry clone containing a V5-tagged mScarlet fused to Giantin (For visualizing the Golgi)

Depositor

Joshua Wythe

Article

Gillespie et al bioRxiv 2024.07.13.603267

Insert

mScarlet3-Giantin

Use

Gateway cloning

Tags

Expression

Mutation

Promoter

Available since

Sept. 27, 2024

Availability

Academic Institutions and Nonprofits only

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MGAT2-RpHLuorin2

Plasmid

#171718

Purpose

Cis-/medial-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to MGAT2 (MGAT2 Human)

Use

Tags

ratiometric pHluorin2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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GalT-RpHLuorin2

Plasmid

#171719

Purpose

Trans-Golgi expression of ratiometric pHLuorin2

Depositor

Geert van den Bogaart

Article

Linders et al ACS Chem Biol. 2022 Jan 21;17(1):240-251

Insert

Ratiometric pHLuorin2 fused to B4GALT1 (B4GALT1 Human)

Use

Tags

ratiometric pHluorin2

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2022

Availability

Academic Institutions and Nonprofits only

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pEF-GalNAc-T2-msGFP2

Plasmid

#140164

Purpose

Expresses the Golgi marker GalnacT2-msGFP2

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

GalNAc-T2-msGFP2 (GALNT2 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCMV-GalT-mCherry

Plasmid

#214271

Purpose

Mammalian expression of GalT-mCherry (Golgi marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

GalT(1-82)-mCherry (B4GALT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pEF-ManII-GFP

Plasmid

#160905

Purpose

Expresses the Golgi marker ManII-GFP in mammalian cells

Depositor

Benjamin Glick

Article

Casler et al Mol Biol Cell. 2020 Oct 28:mbcE20090591.

Insert

MAN2A1 (MAN2A1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

EF-1a

Available since

Nov. 4, 2020

Availability

Academic Institutions and Nonprofits only

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tdEos-VSVG-16

Plasmid

#57693

Purpose

Localization: Golgi/ER Transport, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Article

Shtengel et al Proc Natl Acad Sci U S A. 2009 Mar 3;106

Insert

VSVG

Use

Tags

tdEos

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mRuby2-SiT-N-15

Plasmid

#55912

Purpose

Localization: Golgi TGN, Excitation: 559, Emission: 600

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

SiT (ST6GAL1 Human)

Use

Tags

mRuby2

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available since

March 10, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
Clover-SiT-N-15

Plasmid

#56306

Purpose

Localization: Golgi TGN, Excitation: 505, Emission: 515

Depositor

Michael Davidson

Article

Lam et al Nat Methods. 2012 Sep 9. doi: 10.1038/nm

Insert

SiT (ST6GAL1 Human)

Use

Tags

Clover

Expression

Mammalian

Mutation

aa 1-45 of SiT

Promoter

CMV

Available since

Dec. 5, 2014

Availability

Industry, Academic Institutions, and Nonprofits

Enlarge
mTFP1-ELP-1-25

Plasmid

#55478

Purpose

Localization: Golgi/ER, Excitation: 462, Emission: 492

Depositor

Michael Davidson

Article

Ai et al BMC Biol. 2008 Mar 6;6:13. doi: 10.1186/

Insert

ELP (KDELR2 Human)

Use

Tags

mTFP1

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 10, 2014

Availability

Academic Institutions and Nonprofits only
ECFP-FRB-GiantinS

Plasmid

#210296

Purpose

Golgi marker and anchor for FKBP-FRB CID

Depositor

Takanari Inoue

Article

Nakamura et al Cell Rep. 2023 Sep 2:113089. doi: 10.101

Insert

ECFP-FRB-GiantinS

Use

Tags

ECFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pmScarlet_Giantin_C1

Plasmid

#85048

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Use

Tags

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge
pmScarlet-i_Giantin_C1

Plasmid

#85050

Purpose

In vivo visualization of the Golgi apparatus (can be used for colocalization studies)

Depositor

Dorus Gadella

Article

Bindels et al Nat Methods. 2016 Nov 21. doi: 10.1038/n

Insert

Giantin (GOLGB1 Human)

Use

Tags

Expression

Mammalian

Mutation

aa 3131-3259

Promoter

CMV

Available since

Dec. 6, 2016

Availability

Academic Institutions and Nonprofits only

Enlarge