We narrowed to 7,456 results for: pac

1. pCMV-SpaCas12f1-gRNA_MS13

   Plasmid

   #190877

   Purpose

   The plasmid of CRISPR/SpaCas12f1(Syntrophomonas palmitatica Cas12f1) for genome editing in human cells

   Depositor

   Quanjiang Ji

   Article

   Wang et al Cell Rep. 2022 Sep 27;40(13):111418. doi

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Oct. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAC-Kan-alphaGal4

   Plasmid

   #13421

   Depositor

   Keith Joung

   Article

   Wright et al Nat Protoc. 2006;1(3):1637-52. doi: 10.1

   Insert

   RNAP alpha-Gal4
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Feb. 8, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAcBac1-GFP(39TAG)

   Plasmid

   #217360

   Purpose

   Expression of reporter EGFP with a TAG stop codon at 39 aa position; can be packaged into bacmid

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Nat Methods. 2023 Jan;20(1):95-103. doi:

   Insert

   Enhanced green fluorescent protein
   

   Use

   Baculoviral

   Tags

   Expression

   Insect and Mammalian

   Mutation

   Y39TAG

   Promoter

   CMV

   Available since

   May 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSYN-PACE-001

   Plasmid

   #42503

   Depositor

   Robert Peters

   Article

   Peters et al J Thromb Haemost. 2012 Dec 4. doi: 10.11

   Insert

   soluble PACE (FURIN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   cmv

   Available since

   March 7, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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5. GFP-Epac1 CAAX

   Plasmid

   #118312

   Purpose

   Expression of human Epac1

   Depositor

   Philip Stork

   Article

   Wang et al Mol Cell Biol. 2006 Mar;26(6):2130-45. d

   Insert

   Epac1 CAAX (RAPGEF3 Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   C-terminal 20aa of KiRas

   Promoter

   CMV

   Available since

   Nov. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-mCherry-RspACFAST

   Plasmid

   #216349

   Purpose

   expresses mCherry-RspACFAST fusion cytosolically

   Depositor

   Alison Tebo

   Insert

   mCherry-RspACFAST
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   March 29, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSSRB274_pAC8-eGFP-hsCRBN

   Plasmid

   #218791

   Purpose

   Insect cell expression vector for FLAG-TEV-eGFP-Prescission-hsCRBN

   Depositor

   Eric Fischer

   Article

   Mercer et al Science. 2024 Mar 15;383(6688):eadk4422.

   Insert

   Protein cereblon (CRBN Human)
   

   Use

   Tags

   FLAG-TEV-eGFP-Prescission

   Expression

   Insect

   Mutation

   eGFP fused to N-terminus

   Promoter

   polyhedrin

   Available since

   May 6, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAcGP67A-mMD2-proA

   Plasmid

   #187878

   Purpose

   baculovirus protein expression of mouse MD-2 (residues 19–160) fused to a protein A tag

   Depositor

   Bruce Beutler

   Article

   Wang et al Proc Natl Acad Sci U S A. 2016 Feb 16;11

   Insert

   MD2 (Ly96 Mouse)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   includes residues 19–160 of mouse MD-2

   Promoter

   Available since

   July 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pAc-mCh-Tub

   Plasmid

   #24288

   Depositor

   Ron Vale

   Article

   Vale et al J Cell Biol. 2009 Sep 7. 186(5):727-38.

   Insert

   Alpha-Tubulin (alphaTub84B Fly)
   

   Use

   Tags

   mCherry

   Expression

   Insect

   Mutation

   Promoter

   Available since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

10. pPD937 ZF37x12-Compact_EYFP

    Plasmid

    #138932

    Purpose

    EYFP reporter vector for the ZF37x12-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF37x12-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF37x12-Compact

    Available since

    Feb. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAC90-pmax-DEST

    Plasmid

    #48222

    Purpose

    Gateway Destination vector derived from Clontech's pmax (CAGGS) expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Type

    Empty backbone

    Use

    CRISPR; Gateway destination

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAcBac1-Mb-Fluoro-Phe D6

    Plasmid

    #197577

    Purpose

    Expression of Methanosarcina barkeri (Mb) Fluoro-Phe D6 tRNA synthetase/Pyl-tRNA pair for the encoding of fluorinated phenylalanine derivatives at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Galles et al Nat Commun. 2023 Jan 4;14(1):59. doi: 10

    Inserts

    Mb Pyl Fluoro-Phe B5 synthetase
    Pyl-tRNA (4 copies)
    

    Use

    Tags

    Nuclear export sequence + FLAG

    Expression

    Mammalian

    Mutation

    N311A, C313M, V366G, W382T

    Promoter

    CMV and U6/H1

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pACYC-TtCas6-4xcrRNA4.5

    Plasmid

    #127764

    Purpose

    For expression of T. thermophilus Csm complex in E. coli (crRNA)

    Depositor

    Jennifer Doudna

    Article

    Liu et al Nat Commun. 2019 Jul 5;10(1):3001. doi:

    Inserts

    Synthetic CRISPR array containing four identical T. thermophilus spacers
    Cas6A
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimized for expression in E. coli

    Promoter

    T7

    Available since

    Aug. 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pPD296 ZF43x12-Compact_EYFP

    Plasmid

    #138907

    Purpose

    EYFP reporter vector for the ZF43x12-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x12-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x12-Compact

    Available since

    Feb. 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pPD290 ZF43x6-Compact_EYFP

    Plasmid

    #138901

    Purpose

    EYFP reporter vector for the ZF43x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x6-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x6-Compact

    Available since

    Feb. 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pNH605-PACE-YFP

    Plasmid

    #172002

    Purpose

    Fluorescent reporter for Rpn4 activity

    Depositor

    Johannes Herrmann

    Article

    Boos et al Nat Cell Biol. 2019 Apr;21(4):442-451. d

    Insert

    4xPACE-YFP
    

    Use

    Vector for genomic integration into the yeast gen…

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    CYC1 core promotor with 4 tandem repeats of Rpn4 …

    Available since

    Nov. 2, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pPD548 ZF54x6-Compact_EYFP

    Plasmid

    #138862

    Purpose

    EYFP reporter vector for the ZF54x6-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF54x6-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF54x6-Compact

    Available since

    Jan. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAC1825_pCR8-dCasRx-RBM38

    Plasmid

    #118662

    Purpose

    Gateway Donor; dCasRx-RBM38 ORF; No promoter

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    dCasRx-RBM38
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    No promoter

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAcBac1-Ma-sfGFP 150TAG

    Plasmid

    #197568

    Purpose

    Expression of sfGFP-150TAG with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    sfGFP 150TAG
    M. alvus Pyl-tRNA (6) (4x copies)
    

    Use

    Tags

    V5-His6

    Expression

    Mammalian

    Mutation

    N150TAG

    Promoter

    CMV and U6/H1

    Available since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAcCAST-sgRNA_entry (CJT83)

    Plasmid

    #181785

    Purpose

    Expresses AcCAST. New sgRNA spacer sequences can be added using Golden Gate assembly (via SapI sites).

    Depositor

    Benjamin Kleinstiver

    Article

    Tou et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

    Insert

    AcTnsB, AcTnsC, AcTniQ, AcCas12k
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Lac and J23119

    Available since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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21. pX459-HypaCas9-RHOA_sgRNA

    Plasmid

    #183878

    Purpose

    pX459V2.0-HypaCas9 plasmid with RHOA sgRNA for N-terminal tagging of RhoA in human cells.

    Depositor

    Alisa Piekny

    Article

    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:

    Insert

    RHOA sgRNA spacer (RHOA Human)
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    May 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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22. pAC1821_pCR8-SMN2-DN-RG6-SA

    Plasmid

    #118655

    Purpose

    Transient transfection; Expressed DR-SMN2-DN-RG6-SA-DR CasRx polycistronic gRNA with 3 spacers targeting SMN2 intron and 1 spacer targeting RG6 splice acceptor; Gateway Donor

    Depositor

    Albert Cheng

    Article

    Du et al Nat Commun. 2020 Jun 12;11(1):2973. doi:

    Insert

    SMN2-DN-RG6-SA
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Jan. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pPD292 ZF43x8-Compact_EYFP

    Plasmid

    #138903

    Purpose

    EYFP reporter vector for the ZF43x8-Compact promoter

    Depositor

    Joshua Leonard

    Article

    Donahue et al Nat Commun. 2020 Feb 7;11(1):779. doi: 1

    Insert

    ZF43x8-Compact
    

    Use

    Synthetic Biology

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    ZF43x8-Compact

    Available since

    Feb. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. T7-pAc5.1 mCherry EcoRV

    Plasmid

    #206490

    Purpose

    Schneider cell imaging

    Depositor

    Anne Ephrussi

    Article

    Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

    Type

    Empty backbone

    Use

    Tags

    mCherry

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Sept. 12, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pACAGW-oChiEFtdTomato-AAV

    Plasmid

    #32846

    Purpose

    AAV expression of mammalian codon optimized ChIEF for optogenetic excitation.

    Depositor

    Rory McQuiston

    Article

    Jurgens et al PLoS One. 2012;7(9):e45717. doi: 10.1371

    Insert

    oChiEF
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mutation

    Promoter

    CAGG

    Available since

    March 19, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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26. pACEBac1-gamma-TuSC

    Plasmid

    #178079

    Purpose

    Insect cell expression of gamma-TuSC

    Depositor

    Tarun Kapoor

    Article

    Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

    Inserts

    gamma-tubulin (TUBG1 Human)
    GCP2 (TUBGCP2 Human)
    GCP3 (TUBGCP3 Human)
    

    Use

    Tags

    TEV-His6

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Jan. 31, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAc5.1C-FLuc-V5His6

    Plasmid

    #21183

    Depositor

    Elisa Izaurralde

    Article

    Rehwinkel et al RNA. 2005 Nov . 11(11):1640-7.

    Insert

    Firefly Luciferase
    

    Use

    Drosophila expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 23, 2009

    Availability

    Academic Institutions and Nonprofits only

28. pAc5.1B-EGFP-DmAtx2_AE

    Plasmid

    #148548

    Purpose

    Insect Expression of DmAtx2

    Depositor

    Elisa Izaurralde

    Insert

    DmAtx2 (Atx2 Fly)
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Three silent mutations and two deletions compared…

    Promoter

    Available since

    March 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pAC91-pmax-dCas9VP64

    Plasmid

    #48223

    Purpose

    dCas9VP64 on pmax expression vector

    Depositor

    Rudolf Jaenisch

    Article

    Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

    Insert

    dCas9(D10A;H840A) fusion with VP64 activation domain
    

    Use

    CRISPR

    Tags

    HA Tag and VP64

    Expression

    Mammalian

    Mutation

    D10A;H840A (catalytically inactive)

    Promoter

    CAGGS

    Available since

    Sept. 17, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pAAV-Syn-pAceR

    Plasmid

    #195521

    Purpose

    Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAceR
    

    Use

    AAV

    Tags

    Expression

    Mammalian

    Mutation

    VARNAM 78E, 81D, 92N; SI linker

    Promoter

    Synapsin

    Available since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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