We narrowed to 1,215 results for: tagbfp

1. lenti-Cas9-Exo-Blast-BFP

   Plasmid

   #196721

   Purpose

   Sortable and selectable SpCas9 fused to exodeoxyribonuclease I (sbcB) from E. coli. For the creation of longer deletions.

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-Exo1-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. tRECs/3xUAS-FP attB

   Plasmid

   #220330

   Purpose

   encodes three Fluorescent Protein FLP-OUT cassettes responsive to three different recombinases (mFLP5, B3 and KD)

   Depositor

   Erdem Bangi

   Inserts

   B3RT-STOP-B3RT-TagBFP-3xHA
   KDRT-STOP-KDRT-GFP-HSV
   FRTm71-STOP-FRTm71-mCherry
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   UAS

   Available Since

   Aug. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pYJA5

   Plasmid

   #217778

   Purpose

   The empty vector for quadruple sgRNA cloning

   Depositor

   Adriano Aguzzi

   Article

   Yin et al Nat Biomed Eng. 2024 Dec 4. doi: 10.1038

   Type

   Empty backbone

   Use

   CRISPR, Lentiviral, and Synthetic Biology

   Tags

   Puromycin resistance gene, T2A, and TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   PGK, CMV, U6

   Available Since

   March 11, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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4. pHR hEF1A-BFP-P2A-rtTA-WPRE

   Plasmid

   #223288

   Purpose

   A lentiviral vector expressing BFP and the TetON transcription factor rtTA.

   Depositor

   Jared Toettcher

   Article

   Brumbaugh-Reed et al bioRxiv 2024.01.16.575860

   Insert

   BFP-P2A-rtTA
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 14, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. iC30 PB-Zim3-dCas9-P2A-BFP_hygro

   Plasmid

   #236743

   Purpose

   Constitutive expression of Zim3-dCas9

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI)

   Inserts

   CAG-Zim3-NLS-dCas9-HA-P2A-2xmycNLS-tagBFP-WPRE
   EF1a-hygro-WPRE
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG and EF1a

   Available Since

   May 22, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pHR_5xUAS-ETV2-P2A-BFP-WPRE_PGK-HygR

   Plasmid

   #216664

   Purpose

   Expressed Gal4-inducible ETV2 and BFP, with hygromycin resistance

   Depositor

   Leonardo Morsut

   Inserts

   ETV2 (Etv2 Mouse)
   BFP
   HygromycinR
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAS9-BFP empty

   Plasmid

   #80976

   Purpose

   Cas9, TagBFP, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pMP71-BFP

   Plasmid

   #141355

   Purpose

   pMP71 expression vector containing BFP

   Depositor

   Ton Schumacher

   Article

   Bresser et al Commun Biol. 2020 May 29;3(1):273. doi:

   Insert

   tagBFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   LTR

   Available Since

   June 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pENTR1a-CRISPRoffv2.1

   Plasmid

   #207174

   Purpose

   Gateway Cloning Vector for CRISPRoffv2.1

   Depositor

   David Bersten

   Article

   Allen et al bioRxiv 2023.11.21.568191

   Insert

   CRISPRoffv2.1
   

   Use

   CRISPR

   Tags

   TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJTT002

    Plasmid

    #204493

    Purpose

    Reporter circuit

    Depositor

    Ron Weiss

    Article

    Wauford et al Cell Syst. 2023 Sep 20;14(9):806-818.e5.

    Inserts

    TagBFP
    EYFP
    mKate2
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    hEF1a

    Available Since

    Dec. 19, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-EF1a-BbTagBY

    Plasmid

    #45185

    Depositor

    Joshua Sanes, Dawen Cai

    Article

    Cai et al Nat Methods. 2013 May 5;10(6):540-7. doi

    Has Service

    AAV9

    Insert

    pAAV-EF1a-Brainbow-invert tagBFP-EYFP-WPRE
    

    Use

    AAV; Adeno-associated viral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    human EF1a

    Available Since

    Nov. 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pHR SFFVp BFP-PIF-SOS2cat

    Plasmid

    #50850

    Purpose

    Optogenetic Ras activation system; cytoplasmic component

    Depositor

    Wendell Lim

    Article

    Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

    Inserts

    SOS2cat (SOS2 Human)
    PIF6 1-100
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mutation

    residues 566–1049

    Promoter

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSR49

    Plasmid

    #69259

    Purpose

    phlh-8::tagBFP2

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Insert

    TagBFP2
    

    Use

    Cre/Lox

    Tags

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0, TagBFP2 contains 2 sing…

    Promoter

    hlh-8

    Available Since

    Oct. 14, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono3

    Plasmid

    #73542

    Purpose

    Expression of sgRNA targeting LINE-1 and TagBFP

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    LINE-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV-U6gRNA(BbsI)-PGKpuro2ABFP-L1mono1

    Plasmid

    #73543

    Purpose

    Expression of sgRNA targeting LINE-1 and TagBFP

    Depositor

    Constance Ciaudo

    Article

    Arora et al EMBO Rep. 2022 Jul 20:e54458. doi: 10.15

    Insert

    LINE-1
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    Aug. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. attB-puro donor.dna

    Plasmid

    #181923

    Purpose

    attB-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attB-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLV-EF1a-CRISPRoffv2.1-IRES-BlastR

    Plasmid

    #207180

    Purpose

    Lentiviral Expression Plasmid for CRISPRoff2.1 driven by EF1a Promoter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    CRISPRoffv2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. attP-puro donor

    Plasmid

    #182139

    Purpose

    attP-puro DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. LSM0254 (PB)EF1a-ADAR2-T2A-PuroR-P2A-BFP

    Plasmid

    #233445

    Purpose

    PiggyBac donor plasmid to overexpress a full length ADAR2

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    ADAR2-T2A-PuroR-P2A-BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    EF1a

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV-SFFVp-CRISPRoffv2.1-IRES-BlastR

    Plasmid

    #207181

    Purpose

    Lentiviral Expression Plasmid for CRISPRoff2.1 driven by SFFV Promoter

    Depositor

    David Bersten

    Article

    Allen et al bioRxiv 2023.11.21.568191

    Insert

    CRISPRoffv2.1
    

    Use

    CRISPR and Lentiviral

    Tags

    HA and TagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SFFVp

    Available Since

    April 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. EK0007-PB-4XnrUAS-H2B-BFP

    Plasmid

    #236121

    Purpose

    Fuorescent reporter encoding H2B-tagBFP fusion under control of E1b minimal promoter with four Gal4-binding sites upstream

    Depositor

    Xiaojing Gao

    Article

    Wolfsberg et al bioRxiv 2025.02.24.639918

    Insert

    H2B (H2BC11 Human)
    

    Use

    Tags

    mTagBFP2

    Expression

    Mammalian

    Mutation

    Promoter

    E1b minimal promoter with four Gal4-binding sites…

    Available Since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pSCIPpay-BFP reporter

    Plasmid

    #154096

    Purpose

    Self-Cutting and Integrating Payload CRISPR backbone with BFP reporter

    Depositor

    Scott McComb

    Article

    Bloemberg et al CRISPR J. 2021 Feb;4(1):104-119. doi: 10

    Insert

    P2A-tagBFP
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHR TRE_firefly Luciferase_2A_mCherry-PGK_BFP

    Plasmid

    #223539

    Purpose

    Inducible firefly luciferase and mCherry; constitutive BFP

    Depositor

    Jonathan Brunger

    Article

    Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

    Inserts

    Enhanced firefly luciferase
    mCherry
    TagBFP
    

    Use

    Lentiviral and Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pHR TRE_SEAP_IRES_mCherry-PGK_BFP-hf

    Plasmid

    #223535

    Purpose

    Inducible secreted embryonic alkaline phosphatase and mCherry; constitutive BFP

    Depositor

    Jonathan Brunger

    Article

    Walton et al Osteoarthritis Cartilage. 2025 Feb;33(2)

    Inserts

    Secreted alkaline phosphatase
    mCherry
    TagBFP
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    June 17, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pk335-BFP

    Plasmid

    #227505

    Purpose

    Backbone for CARGO cloning, with U6 promoter and gRNA scaffold, TagBFP, and Kanamycin resistence

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. LSM061 (PB)SFFV-LIDARsensor(mcherry-BFP) (hygR)

    Plasmid

    #233427

    Purpose

    PiggyBac donor plasmid with SFFV driven LIDAR reporter RNA (mCherry as marker, TagBFP as output)

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    LIDARsensor(mcherry-BFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. FRB-BFP-GCN4-ppKin14

    Plasmid

    #240426

    Purpose

    -end oriented kinesin for rapalog induced pulling experiments

    Depositor

    Anna Akhmanova

    Article

    Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

    Insert

    ppKin14Vib
    

    Use

    Tags

    FRB, GCN4, and tagBFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-850

    Promoter

    Available Since

    July 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pSB-TRE-CRISPRoff-EF1A-TetOn

    Plasmid

    #203355

    Purpose

    Drug-inducible expression of CRISPRoff components needed for DNA methylation and gene repression with Sleeping Beauty backbone

    Depositor

    Jonathan Brunger

    Article

    Gil et al Bio Protoc. 2024 Jan 5;14(1):e4913. doi:

    Insert

    CRISPRoff-TetOn
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 17, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pUC CBA-SpCas9.EF1a-BFP.sgLMNA

    Plasmid

    #98971

    Purpose

    Cas9 Homologous Recombination Reporter. SpCas9 and a sgRNA targeting LMNA. TagBFP.

    Depositor

    Jan Karlseder

    Article

    Arnoult et al Nature. 2017 Sep 20;549(7673):548-552. d

    Insert

    LMNA sgRNA and spCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 11, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pDV24_AAV2_DART-VADAR_iRFP720-sensor

    Plasmid

    #200411

    Purpose

    AAV vector encoding a DART-VADAR sensor for iRFP720 mRNA

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    DART VADAR sensor
    

    Use

    AAV and Synthetic Biology

    Tags

    MCP-ADAR2dd(E488Q)-NES, TagBFP, and mNeonGreen

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. CMVTO-hIL2

    Plasmid

    #233387

    Purpose

    secreted human IL-2

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    IL-2 (IL2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    WT

    Promoter

    CMV

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pHR SFFVp BFP-Erk2

    Plasmid

    #50848

    Purpose

    Fluorescent reporter for Erk nuclear translocation

    Depositor

    Wendell Lim

    Article

    Toettcher et al Cell. 2013 Dec 5;155(6):1422-34. doi: 10

    Insert

    Erk2 (Mapk1 Mouse)
    

    Use

    Lentiviral

    Tags

    TagBFP

    Expression

    Mutation

    Promoter

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. attB-puro-GA donor

    Plasmid

    #182138

    Purpose

    attB-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attB-GA-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. attP-puro-GA donor

    Plasmid

    #182140

    Purpose

    attP-puro-GA DNA donor for twinPE and Bxb1 mediated gene-size insertion

    Depositor

    David Liu

    Article

    Anzalone et al Nat Biotechnol. 2022 May;40(5):731-740.

    Insert

    attP-GA-EGFP-EF1α-PuroR-T2A-TagBFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pMALB

    Plasmid

    #161784

    Purpose

    Gateway destination vector with bidirectional CMV-PGK promoter for modest expression of gene of interest and TagBFP.

    Depositor

    John Dick, Peter van Galen

    Article

    van Galen et al Cell Stem Cell. 2014 Jan 2;14(1):94-106.

    Type

    Empty backbone

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Bidirectional minhCMV and hPGK

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pBC155

    Plasmid

    #202274

    Purpose

    Level 2 for strain-specific barcode DNA tag with bc182 through Tn7 bacterial insertion, Spectinomycin and Streptomycin selectable marker and tagBFP fluorescent marker

    Depositor

    Paul Schulze-Lefert

    Article

    Ordon et al Nat Microbiol. 2024 Apr;9(4):1117-1129.

    Insert

    bc182
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    April 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. LSM0252 (PB)EF1a-FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP

    Plasmid

    #233443

    Purpose

    PiggyBac donor plasmid to overexpress FKBP-ADAR2dd (cLIDAR)

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    FKBP-ADAR2dd-IRESv0-PuroR-P2A-BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available Since

    June 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pk335-NT-1mer-gRNA

    Plasmid

    #227500

    Purpose

    non-targeting control guide

    Depositor

    Joanna Wysocka

    Article

    Jensen et al Mol Cell. 2025 Jan 16;85(2):347-361.e7.

    Insert

    Non-targeting gRNA
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    May 5, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. LSM0258 (PB)EF1a-ADAR2dd-PCP-T2A-PuroR-P2A-BFP

    Plasmid

    #233446

    Purpose

    PiggyBac donor plasmid to overexpress ADAR2dd-PCP

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    ADAR2dd-PCP-T2A-PuroR-P2A-BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available Since

    April 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. LSM0251 (PB)EF1a-FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP

    Plasmid

    #233442

    Purpose

    PiggyBac donor plasmid to overexpress FKBP-TM-ADAR2dd (eLIDAR)

    Depositor

    Xiaojing Gao

    Article

    Zhang et al Nat Chem Biol. 2025 Mar 28. doi: 10.1038

    Insert

    FKBP-tm-ADAR2ddIRESv0-PuroR-P2A-BFP
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    E488Q, T501A on ADAR2dd

    Promoter

    EF1a

    Available Since

    April 1, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge