We narrowed to 1,231 results for: vent

1. pcsII-pb6-flag

   Plasmid

   #86770

   Purpose

   C-terminal flag-tagged protein expression in mammalian cells, lentiviral vector

   Depositor

   Do-Hyung Kim

   Article

   Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

   Insert

   proteasome beta subunit 6 (PSMB6 Human)
   

   Use

   Lentiviral

   Tags

   flag

   Expression

   Mammalian

   Mutation

   Promoter

   unknown

   Available since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pCDH-3xFLAG-hTCAB1-puroR

   Plasmid

   #167459

   Purpose

   3rd-gen lentiviral expression of FLAG-TCAB1 (WDR79) in mammalian cells

   Depositor

   Steven Artandi

   Article

   Chen et al Cell. 2018 Jun 28;174(1):218-230.e13. do

   Insert

   TCAB1 (WRAP53 Human)
   

   Use

   Lentiviral

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRSFDuet-1 IntS11 AA 300-597

   Plasmid

   #199329

   Purpose

   Expresses His-tagged Drosophila IntS11 amino acids 300-597

   Depositor

   Jeremy Wilusz

   Article

   Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

   Insert

   Integrator subunit 11 (IntS11 Fly)
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   amino acids 300-597 only

   Promoter

   Available since

   Nov. 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pFastBac_Dual_GST-TEV-EGFP-TBK1

   Plasmid

   #187830

   Purpose

   Plasmid for the expression and purification of GST-TEV-EGFP-TBK1 in Spodoptera frugiperda cells (Sf9). Internal reference: SMC1631

   Depositor

   Sascha Martens

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   TBK1 (TBK1 Human)
   

   Use

   Tags

   GST and TEV-mEGFP-GG Linker

   Expression

   Bacterial and Insect

   Mutation

   Promoter

   Available since

   Oct. 20, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pLenti-CMV-DNAJC5 (L115R)

   Plasmid

   #205728

   Purpose

   Mammalian expression of DNAJC5 (L115R)

   Depositor

   Randy Schekman

   Article

   Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

   Insert

   DNAJC5 (L115R) (DNAJC5 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   L115R

   Promoter

   Available since

   Jan. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. mEos3.2trCD4o

   Plasmid

   #203751

   Purpose

   Encodes the transmembrane domain from CD4, including its 2 palmitoylation sites, with mEos3.2 fused on the N terminus. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trCD4o (CD4 Human)
   

   Use

   Tags

   mEos3.2

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pEMS1725

   Plasmid

   #105870

   Purpose

   High copy number plasmid containing iCreERT2-FFrt-KanR-FFrt insert

   Depositor

   Elizabeth Simpson

   Article

   Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

   Insert

   iCreERT2-FFrt-KanR-FFrt
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pBMN HA-ATG13

   Plasmid

   #186223

   Purpose

   For expression of ATG13 tagged with HA

   Depositor

   Michael Lazarou

   Article

   Nguyen et al Mol Cell. 2023 May 18;83(10):1693-1709.e

   Insert

   ATG13 (ATG13 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 23, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. mEos3.2trLAT-2P

   Plasmid

   #203748

   Purpose

   Encodes the transmembrane domain from mouse LAT with mEos3.2 fused on the N terminus. Palmitoylation sites at C27 and C30 mutated to A. Used as a fluorescent plasma membrane probe.

   Depositor

   Sarah Veatch

   Article

   Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d

   Insert

   trLAToC27AC30A (Lat Mouse)
   

   Use

   Tags

   ER translocation signal - mEos3.2

   Expression

   Mammalian

   Mutation

   C27A, C30A

   Promoter

   Available since

   Aug. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pTRE Tight ArchT-GFP

    Plasmid

    #79627

    Purpose

    Tetracycline-dependent expression of ArchT-EGFP in mammalian cells

    Depositor

    Yasunori Hayashi

    Article

    Sakaguchi et al PLoS One. 2015 Jun 15;10(6):e0130163. do

    Insert

    ArchT
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    ArchT is a more sensitive version of Arch (archae…

    Promoter

    Tet responsive Ptight promoter

    Available since

    July 1, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pCFJ889

    Plasmid

    #84826

    Purpose

    Minimos transposon with Psmu-1:smu-1:GFP:smu-1 UTR and cbr-unc-119 selection

    Depositor

    Andrew Fire

    Article

    Frokjaer-Jensen et al Cell. 2016 Jul 14;166(2):343-57. doi: 10

    Insert

    smu-1 (smu-1 Nematode)
    

    Use

    Tags

    GFP

    Expression

    Bacterial and Worm

    Mutation

    Promoter

    Psmu-1

    Available since

    Jan. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. 3389 pcDNA3 Bid p22

    Plasmid

    #8807

    Depositor

    Stanley Korsmeyer

    Article

    Gross et al J Biol Chem 1999 Jan 8;274(2):1156-63.

    Insert

    Bid p22 (Bid Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2006

    Availability

    Academic Institutions and Nonprofits only

13. 3392 pcDNA3 Bid p15

    Plasmid

    #8808

    Depositor

    Stanley Korsmeyer

    Article

    Gross et al J Biol Chem 1999 Jan 8;274(2):1156-63.

    Insert

    Bid p15 (Bid Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

14. pEMS1925

    Plasmid

    #105869

    Purpose

    High copy number plasmid containing iCreERT2 synthesized fragment for constructing the HPRT plasmid

    Depositor

    Elizabeth Simpson

    Article

    Korecki et al Genetics. 2019 Feb 14. pii: genetics.119

    Insert

    iCreERT2
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. HA-PRAS40 F129A

    Plasmid

    #86763

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    human PRAS40 (AKT1S1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    F129A

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. HA-PRAS40 W10A

    Plasmid

    #86761

    Purpose

    N-terminal HA-tagged protein expression in mammalian cells

    Depositor

    Do-Hyung Kim

    Article

    Yun et al Mol Cell. 2016 Feb 18;61(4):625-39. doi:

    Insert

    human PRAS40 (AKT1S1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    W10A

    Promoter

    CMV

    Available since

    Jan. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. 3405 pBM272 Bid p22

    Plasmid

    #8771

    Depositor

    Stanley Korsmeyer

    Article

    Gross et al J Biol Chem 1999 Jan 8;274(2):1156-63.

    Insert

    Bid p22 (Bid Mouse)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 6, 2005

    Availability

    Academic Institutions and Nonprofits only

18. pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT

    Plasmid

    #238281

    Purpose

    For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P2AT2A-EGFP-BRD4-NUT
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. mscv_EGFP-IRES-NUP98-KDM5A-KS-FtoG

    Plasmid

    #238292

    Purpose

    For overexpression of EGFP-IRES-NUP98-KDM5A-KS-FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EGFP-IRES-NUP98-KDM5A-KS-FtoG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

    Plasmid

    #238286

    Purpose

    For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P2AT2A-GFP-NUP98-DDX10-KS-FtoG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. TRE3G-mCherry-P2A-T2A-nanobody-KS-FtoA

    Plasmid

    #238288

    Purpose

    For integration of mCherry-P2A-T2A-nanobody-KS-FtoA

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P2A-T2A-nanobody-KS-FtoA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG

    Plasmid

    #238283

    Purpose

    For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P2AT2A-EGFP-BRD4-NUT-KS-FtoG
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pRK5_mCherry-P2AT2A-EGFP-BRD4-NUT-KS

    Plasmid

    #238282

    Purpose

    For overexpression of mCherry-P2AT2A-EGFP-BRD4-NUT-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P2AT2A-EGFP-BRD4-NUT-KS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10

    Plasmid

    #238284

    Purpose

    For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P2AT2A-GFP-NUP98-DDX10
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pX458_mCherry-NPM1-gRNA for HCT116

    Plasmid

    #238249

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to NPM1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NPM1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pRK5_mCherry-P2AT2A-GFP-NUP98-DDX10-KS

    Plasmid

    #238285

    Purpose

    For overexpression of mCherry-P2AT2A-GFP-NUP98-DDX10-KS

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    mCherry-P2AT2A-GFP-NUP98-DDX10-KS
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pX458_mCherry-NPM1-gRNA for U2OS

    Plasmid

    #238250

    Purpose

    Cas9 from S. pyogenes with 2A-mCherry, gRNA to knock-in msfGFP to NPM1 locus

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    NPM1
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. mscv_EGFP-IRES-NUP98-KDM5A-KS-FtoA

    Plasmid

    #238291

    Purpose

    For overexpression of EGFP-IRES-NUP98-KDM5A-KS-FtoA

    Depositor

    Denes Hnisz

    Article

    Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

    Insert

    EGFP-IRES-NUP98-KDM5A-KS-FtoA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 3, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. p300dAIL KAT LIC 1M

    Plasmid

    #233588

    Purpose

    Bacterial expression of p300 KAT enzyme with truncated autoinhibitory loop

    Depositor

    Scott Showalter

    Article

    Dewing et al Proteins. 2025 Jun 1. doi: 10.1002/prot.

    Insert

    p300dAIL KAT (EP300 Human)
    

    Use

    Tags

    6xHis MBP

    Expression

    Bacterial

    Mutation

    deleted amino acids 1523-1554

    Promoter

    T7

    Available since

    June 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. AAV-DIO-Bmal1

    Plasmid

    #239045

    Purpose

    Overexpression of Bmal1

    Depositor

    Qingchun Tong

    Article

    Kim et al Nat Commun. 2020 Jul 30;11(1):3794. doi:

    Insert

    mArntl (Bmal1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Bmal1

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pETcon-pGAL1-Aga2-Htt(25Q)-c_myc

    Plasmid

    #225228

    Purpose

    Yeast optimized human Htt (25Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (25Q) in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

    Inserts

    Aga2
    Htt (25Q)
    

    Use

    Tags

    HA Tag and c-myc Tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

32. pETcon-pGAL1-Aga2-Htt(46Q)-c_myc

    Plasmid

    #225229

    Purpose

    Yeast optimized human Htt (46Q) fused with aga2 protein gene under gal1 promoter for the expression of Htt (46Q) in yeast surface display.

    Depositor

    Urartu Seker

    Article

    Khan et al ACS Med Chem Lett. 2023 Nov 14;14(12):18

    Inserts

    Aga2
    Htt (46Q)
    

    Use

    Tags

    HA Tag and c-myc Tag

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 16, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-DNAJC5-ΔC30 (Δ169-198)

    Plasmid

    #205722

    Purpose

    Mammalian expression of DNAJC5-ΔC30 (Δ169-198)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ169-198

    Promoter

    Available since

    Feb. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

34. pCMV-XPACK-DNAJC5 (L115R)

    Plasmid

    #205717

    Purpose

    Mammalian expression of XPACK-DNAJC5 (L115R)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    XPACK (GCINSKRKD)

    Expression

    Mammalian

    Mutation

    L115R

    Promoter

    Available since

    Jan. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

35. pCMV-XPACK-DNAJC5 (WT)

    Plasmid

    #205716

    Purpose

    Mammalian expression of XPACK-DNAJC5 (WT)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    XPACK (GCINSKRKD)

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-DNAJC5-ΔC10 (Δ189-198)

    Plasmid

    #205720

    Purpose

    Mammalian expression of DNAJC5-ΔC10 (Δ189-198)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ189-198

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-DNAJC5-ΔC20 (Δ179-198)

    Plasmid

    #205721

    Purpose

    Mammalian expression of DNAJC5-ΔC20 (Δ179-198)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ179-198

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-DNAJC5-ΔC40 (Δ151-198)

    Plasmid

    #205723

    Purpose

    Mammalian expression of DNAJC5-ΔC40 (Δ151-198)

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Δ151-198

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

39. pCMV-DNAJC5 (L115R)-HaloTag

    Plasmid

    #205711

    Purpose

    Mammalian expression of DNAJC5 (L115R)-HaloTag

    Depositor

    Randy Schekman

    Article

    Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

    Insert

    DNAJC5 (DNAJC5 Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    L115R

    Promoter

    Available since

    Jan. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

40. pMtnA FLAG-IntS7 puro

    Plasmid

    #208405

    Purpose

    Expresses FLAG-tagged Drosophila IntS7 from inducible MtnA promoter

    Depositor

    Jeremy Wilusz

    Article

    Fujiwara et al Mol Cell. 2023 Nov 14:S1097-2765(23)0090

    Insert

    pMT FLAG MCS puro (defl Fly)
    

    Use

    Tags

    3xFLAG

    Expression

    Insect

    Mutation

    Promoter

    MtnA

    Available since

    Dec. 8, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge