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We narrowed to 1,287 results for: MS2

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Showing: 901 - 950 of 1287 results

  7. pCH6 - pBait-ChiX

    Plasmid
    #174662
    Purpose
    arabinose-induced synthesis of a hybrid RNA containing a single MS2 hairpin followed by E coli ChiX inserted between XmaI and HindIII sites. Insert can be replaced with RNA bait of interest.
    Depositor
    Katherine Berry
    Article
    Pandey et al Nucleic Acids Res. 2020 May 7;48(8):4507
    Insert
    ChiX
    Tags
    1xMS2 hairpin
    Expression
    Bacterial
    Promoter
    pBad
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    174662-plasmid-map-sequence-id-361921

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  20. THI ONLY pALD4 T-

    Plasmid
    #161497
    Purpose
    Non activation and non targeting control for CRISPR/RNA scaffold based transcription regulation in Pichia pastoris BB3rN_pTEF2_dCAS9(3xFLAG_SV40NLS)_pPOR1_MS2bind_VP64_SV40NLS_pGAP_THI11_T-_gRNA_MS2
    Depositor
    Brigitte Gasser, Diethard Mattanovich
    Article
    Baumschabl et al ACS Synth Biol. 2020 Dec 18;9(12):3202-3
    Inserts
    dCAS9
    MS2 (non activation control NAC)
    gRNA (Non targeting control NTC)
    Expression
    Yeast
    Promoter
    pALD4, pPOR1, and pTEF2
    Available Since
    March 15, 2021
    Availability
    Academic Institutions and Nonprofits only
    161497-plasmid-map-sequence-id-326273

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  24. lentiSAMv2

    Plasmid
    #75112
    Purpose
    lenti sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2, dCas9-VP64, and blast resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Feng Zhang
    Article
    Joung et al Nat Protoc. 2017 Apr;12(4):828-863. doi:
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 and EF1A
    Available Since
    May 24, 2016
    Availability
    Academic Institutions and Nonprofits only
    75112-plasmid-map-sequence-id-305764

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  25. PB-UniSAM

    Plasmid
    #99866
    Purpose
    Encodes for Cas9-VP64, MS2-p65-HSF1, mCherry and for the gRNA 2.0
    Depositor
    Lesley Forrester
    Article
    Fidanza et al Sci Rep. 2017 Jul 25;7(1):6394. doi: 10.
    Insert
    UniSAM-mCherry + U6-gRNA2.0
    Use
    CRISPR and Synthetic Biology; Dcas9-sam activation
    Expression
    Mammalian
    Mutation
    BbsI sites in CDS were ablated by consensus mutat…
    Promoter
    EF1a
    Available Since
    Aug. 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    99866-plasmid-map-sequence-id-192399

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  29. pCMV-scFv-GCN4-StayGold-IRESv4-HaloTag-tdMCP-CAAX

    Plasmid
    #241144
    Purpose
    Expresses Anti-GCN4 scFv-StayGold, and HaloTag-tdMCP-CAAX to track mature and nascent HA-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-GCN4 scFv
    tdMCP - CAAX
    Tags
    (n2)oxStayGold(c4) E147D and HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    241144-plasmid-map-sequence-id-485248

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  30. pYPQAT27

    Plasmid
    #223399
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by 2x35s and the sgRNA was driven by AtU3; Kanamycin for plant select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    2x35s-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223399-plasmid-map-sequence-id-452308

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  31. pYPQAT25

    Plasmid
    #223397
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by AtUBQ10 and sgRNA was driven by AtU3; Hygromycin for plants select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    AtUBQ10-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223397-plasmid-map-sequence-id-453300

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  32. pHAGE UbiC NLS-HA-stdMCP-stdHalo-RH1

    Plasmid
    #164614
    Purpose
    Lentiviral vector expressing synonymous tandem MS2 coat protein fused to synonymous tandem Halo-tag and the RH1 domain of RILPL2. Used for single-molecule imaging of actin-anchored mRNA.
    Depositor
    Jeffrey Chao
    Article
    Mateju et al Cell. 2020 Dec 23;183(7):1801-1812.e13.
    Insert
    stdMCP-stdHalo-RH1
    Use
    Lentiviral
    Tags
    HA tag and NLS
    Expression
    Bacterial and Mammalian
    Promoter
    UbiC
    Available Since
    Feb. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    164614-plasmid-map-sequence-id-325003

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  36. pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-CAAX

    Plasmid
    #241145
    Purpose
    Expresses Anti-HA frankenbody-StayGold and HaloTag-tdMCP-CAAX to track mature and nascent SunTag-tagged proteins and track MS2-tagged mRNA anchored to the plasma membrane
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-HA frankenbody
    tdMCP - CAAX
    Tags
    (n2)oxStayGold(c4) E147D and HaloTag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 8, 2025
    Availability
    Academic Institutions and Nonprofits only
    241145-plasmid-map-sequence-id-485242

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  37. pYPQAT26

    Plasmid
    #223398
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for dicot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by AtU3; BASTA for plants select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-AtU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223398-plasmid-map-sequence-id-452310

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  38. pYPQAT28

    Plasmid
    #223400
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and sgRNA was driven by OsU3; Hygromycin for plant select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223400-plasmid-map-sequence-id-452334

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  39. pYPQAT29

    Plasmid
    #223401
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 was driven by ZmUbi1 and the sgRNA was driven by OsU3; BASTA for plant select.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-OsU3-gRNA scaffold 2.0
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223401-plasmid-map-sequence-id-452216

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  40. pYPQAT30

    Plasmid
    #223402
    Purpose
    T-DNA vector for PmCDA1-CBE_V04 based C-to-T base editing for monocot plants; NGG PAM; 5' editing window; PmCDA1-CBE_V04 and the sgRNA was driven by separate ZmUbi1; Hygromycin for plants selection.
    Depositor
    Yiping Qi
    Article
    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.
    Insert
    ZmUbi-SpCas9D10A-PmCDA-UGI-T2A-MS2-UGI-ZmUbi-gRNA scaffold 2.0-tRNA
    Use
    CRISPR
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Available Since
    April 10, 2025
    Availability
    Academic Institutions and Nonprofits only
    223402-plasmid-map-sequence-id-454247

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  43. lentiSAM v2 (Puro)

    Plasmid
    #92062
    Purpose
    Modified version of lentiSAM v2, a lenti sgRNA cloning backbone with MS2 loops at tetraloop/stemloop 2, dCas9-VP64, and puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.
    Depositor
    Adam Karpf
    Article
    Barger et al Elife. 2021 Apr 23;10. pii: 55070. doi:
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6 (sgRNA) and EF1a (dCas9-VP64)
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    92062-plasmid-map-sequence-id-180159

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  44. pCMV-Frankenbody-HA-StayGold-IRESv4-HaloTag-tdMCP-tdP2A-tdPCP-mCherry

    Plasmid
    #241142
    Purpose
    Expresses Anti-HA frankenbody-StayGold, HaloTag-tdMCP, and tdPCP-mCherry to track mature and nascent HA-tagged proteins and track MS2 and PP7-tagged mRNA
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-HA frankenbody
    tdMCP
    tdPCP
    Tags
    (n2)oxStayGold(c4) E147D, 3x mCherry, and HaloTag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    241142-plasmid-map-sequence-id-487015

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  45. pCMV-Frankenbody-HA-mScarlet3-tdP2A-scFv-GCN4-sfGFP-IRESv4-HaloTag-tdMCP

    Plasmid
    #241139
    Purpose
    Expresses Anti-HA frankenbody-mScarlet3, anti-GCN4 scfv-sfGFP, and HaloTag-tdMCP to track mature and nascent HA and SunTag-tagged proteins and MS2 tagged mRNA
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-HA frankenbody
    anti-GCN4 scfv
    tdMCP
    Tags
    HaloTag, mScarlet3, and sfGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    241139-plasmid-map-sequence-id-485247

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  49. pCMV-scFv-GCN4-StayGold-IRESv4-HaloTag-tdMCP-tdP2A-tdPCP-mCherry

    Plasmid
    #241143
    Purpose
    Expresses Anti-GCN4 scFv-StayGold, HaloTag-tdMCP, and tdPCP-mCherry to track mature and nascent SunTag-tagged proteins and track MS2 and PP7-tagged mRNA
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-GCN4 scFv
    tdMCP
    tdPCP
    Tags
    (n2)oxStayGold(c4) E147D, 3x mCherry, and HaloTag…
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Oct. 21, 2025
    Availability
    Academic Institutions and Nonprofits only
    241143-plasmid-map-sequence-id-487014

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  50. pCMV-scFv-GCN4-sfGFP-tdP2A-Frankenbody-FLAG-mScarlet3-IRESv4-HaloTag-tdMCP

    Plasmid
    #241141
    Purpose
    Expresses Anti-GCN4 scFv-sfGFP, anti-FLAG frankenbody-mEGFP, and HaloTag-tdMCP to track mature and nascent SunTag and FLAG-tagged proteins and MS2-tagged mRNA
    Depositor
    Tim Stasevich
    Article
    Galindo et al Mol Biol Cell. 2025 Jun 1;36(6):mr6. doi
    Inserts
    Anti-GCN4 scFv
    anti-FLAG frankenbody
    tdMCP
    Tags
    HaloTag, mEGFP, and sfGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Sept. 3, 2025
    Availability
    Academic Institutions and Nonprofits only
    241141-plasmid-map-sequence-id-485239

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Showing: 901 - 950 of 1287 results