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We narrowed to 1,593 results for: PTS

Showing: 901 - 950 of 1593 results

  1. NK651

    Plasmid
    #109107
    Purpose
    Reporter gene expression in S. rosetta
    Depositor
    Nicole King
    Article
    Booth et al Mol Biol Cell. 2018 Dec 1;29(25):3026-30
    Insert
    PTSG_07215 deltaCC
    Use
    Choanoflagellate expression
    Tags
    Expression
    Mutation
    Deletes C-terminal coiled-coil from PTSG_07215
    Promoter
    Available since
    July 10, 2018
    Availability
    Academic Institutions and Nonprofits only
    109107-plasmid-map-sequence-id-210565

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  9. pDY279

    Plasmid
    #182959
    Purpose
    Str resistant,CoE1* ori. CRISPR/Cas9 gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations in ptsI. (for 1st round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (ttcaggcattttagcatccc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182959-plasmid-map-sequence-id-365361

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  10. pDY281

    Plasmid
    #182960
    Purpose
    Amp resistant, CoE1* ori. CRISPR/Cas9 induced by AHTc, gRNA under constitutive J23119 promoter. gRNA targeting E. coli ptsI codon 2~9. HRT has synonymous mutations of ptsI. (for 2nd round editing)
    Depositor
    Shelley Copley
    Article
    Yang et al Proc Natl Acad Sci U S A. 2024 Jan 30;12
    Insert
    gRNA (acctggtgaagccaatattc), homologous repair template for ptsI
    Use
    Synthetic Biology
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Jan. 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    182960-plasmid-map-sequence-id-365357

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  11. HDM_SARS2_Spike_del21_D614G

    Plasmid
    #158762
    Purpose
    Mammalian expression vector for expressing SARS-2 Spike with 21 aa del at C-terminus and pt mutation D to G at site 614
    Depositor
    Jesse Bloom
    Article
    Crawford et al Viruses. 2020 May 6;12(5). pii: v1205051
    Insert
    SARS2-Spike_del21_D614G (S Severe acute respiratory syndrome coronavirus 2, Synthetic)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    deleted amino acids 1257-1278, changed aspartic a…
    Promoter
    CMV
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    158762-plasmid-map-sequence-id-309495

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  12. pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus

    Plasmid
    #105127
    Purpose
    Used along with I-SceI enzyme to generate transgenic zebrafish expressing hepatocyte-specific Xenopus activated beta-catenin and lens-specific Venus fluorescent protein
    Depositor
    Kimberley Evason, Didier Stainier
    Article
    Evason et al PLoS Genet. 2015 Jul 2;11(7):e1005305. d
    Inserts
    Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence (cryaa Zebrafish)
    Use
    Zebrafish transgenesis
    Tags
    Expression
    Mutation
    Promoter
    cryaa (crystallin) and fabp10a
    Available since
    April 17, 2019
    Availability
    Academic Institutions and Nonprofits only
    105127-plasmid-map-sequence-id-220518

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  13. pUC19_mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP

    Plasmid
    #195505
    Purpose
    T-REX17 donor plasmid to generate locus specific integration of mRuby followed by 3xSV40 poly(A) into Exon 1 of human lncRNA T-REX17
    Depositor
    Alexander Meissner
    Article
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    pT-REX-mRuby-3xFLAG-NLS-3xSV40-poly(A)_loxP-PGK-Puro-loxP_T-REX(Ex1)
    Use
    CRISPR; Donor vector
    Tags
    mRuby-3xFLAG-NLS-3xSV40-poly(A)
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195505-plasmid-map-sequence-id-384991

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  14. pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL

    Plasmid
    #73210
    Purpose
    Expresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP)
    Depositor
    Marcel Bruchez
    Article
    Telmer et al ACS Chem Biol. 2015 May 15;10(5):1239-46
    Insert
    KozATG-myc-dL5-2XG4S-mCer3-SKL (MYC Human, Synthetic)
    Use
    Tags
    The FAP and mCerulean3 are fused with 2 copies of…
    Expression
    Mammalian
    Mutation
    The dL5** FAP is E50D, L89S, also known as E52D/L…
    Promoter
    CMV
    Available since
    March 9, 2016
    Availability
    Academic Institutions and Nonprofits only
    73210-plasmid-map-sequence-id-139746

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  15. mEmerald-Peroxisomes-2

    Plasmid
    #54228
    Purpose
    Localization: Peroxisomes, Excitation: 487, Emission: 509
    Depositor
    Michael Davidson
    Insert
    Peroximal Targeting Signal 1 (PTS1)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 10, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  18. TDP43-NTD

    Plasmid
    #241024
    Purpose
    Bacterial expression of TDP-43 NTD
    Depositor
    Nicolas Fawzi
    Insert
    TDP43-NTD
    Use
    Tags
    6x His
    Expression
    Bacterial
    Mutation
    changed N81 to stop codon
    Promoter
    Available since
    July 24, 2025
    Availability
    Academic Institutions and Nonprofits only
    241024-plasmid-map-sequence-id-479758

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  20. pEYFP-N1-HsSmg5-del267-809_I

    Plasmid
    #146538
    Purpose
    Mammalian Expression of HsSmg5-del267-809
    Depositor
    Elisa Izaurralde
    Insert
    HsSmg5-del267-809 (SMG5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    one non silent mutation A254G (K85R) compared to …
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146538-plasmid-map-sequence-id-288984

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  21. pEYFP-N1-HsSmg5-siRNAres_I

    Plasmid
    #146541
    Purpose
    Mammalian Expression of HsSmg5-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsSmg5-siRNAres (SMG5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    three non silent mutation A254G (K85R), T980C (L3…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146541-plasmid-map-sequence-id-288987

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  22. pCIneo-lambdaN-HA-HsSmg5_I

    Plasmid
    #146531
    Purpose
    Mammalian Expression of HsSmg5
    Depositor
    Elisa Izaurralde
    Insert
    HsSmg5 (SMG5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    three non silent mutation A254G (K85R), T980C (L3…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146531-plasmid-map-sequence-id-288977

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  30. pEYFP-N1-HsSmg5-del13-266-siRNAres_I

    Plasmid
    #146537
    Purpose
    Mammalian Expression of HsSmg5-del13-266-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsSmg5-del13-266-siRNAres (SMG5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    two non silent mutation, T980C (L327P), T1084C (C…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146537-plasmid-map-sequence-id-288983

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  39. pEYFP-N1-HsSmg5-del810-846-siRNAres_I

    Plasmid
    #146539
    Purpose
    Mammalian Expression of HsSmg5-del810-846-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsSmg5-del810-846-siRNAres (SMG5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    three non silent mutation A254G (K85R), T980C (L3…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146539-plasmid-map-sequence-id-288985

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  40. pEYFP-N1-HsSmg5-del847-1017-siRNAres_I

    Plasmid
    #146540
    Purpose
    Mammalian Expression of HsSmg5-del847-1017-siRNAres
    Depositor
    Elisa Izaurralde
    Insert
    HsSmg5-del847-1017-siRNAres (SMG5 Human)
    Use
    Tags
    Expression
    Mammalian
    Mutation
    three non silent mutation A254G (K85R), T980C (L3…
    Promoter
    Available since
    Jan. 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    146540-plasmid-map-sequence-id-288986

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  47. pCLAP

    Plasmid
    #200786
    Purpose
    Cloning template for N-terminal CLIPf tagging and C-terminal SNAPf tagging of constructs for mammalian expression. Also used for the mammalian expression of CLIPf-SNAPf-HisTag-FLAG fusion protein.
    Depositor
    Alison Twelvetrees
    Article
    Smith et al bioRxiv 2024.12.20.629623
    Type
    Empty backbone
    Use
    Tags
    CLIPf and SNAPf-6xHis-FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Dec. 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    200786-plasmid-map-sequence-id-435692

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Showing: 901 - 950 of 1593 results