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Showing: 901 - 950 of 4900 results

  3. Padh1-MCS-TADH1-Sp.arg3+/pUC19*

    Plasmid
    #169349
    Purpose
    Expression in S. pombe from adh1 promoter, integrated at arg3-D4 locus
    Depositor
    Tony Carr
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169349-plasmid-map-sequence-id-332962

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  4. Padh81-MCS-TADHdh1-Sp.arg3+/pUC19*

    Plasmid
    #169351
    Purpose
    Expression in S. pombe from adh81 promoter, integrated at arg3-D4 locus
    Depositor
    Tony Carr
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169351-plasmid-map-sequence-id-332961

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  5. Padh15-MCS-TADH1-Sp.arg3+/pUC19*

    Plasmid
    #169350
    Purpose
    Expression in S. pombe from adh15 promoter, integrated at arg3-D4 locus
    Depositor
    Tony Carr
    Type
    Empty backbone
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169350-plasmid-map-sequence-id-332956

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  8. pUC19-U6-BsmBI_cassette-St1Cas9-sgRNA (KAC14)

    Plasmid
    #133791
    Purpose
    U6 promoter sgRNA entry vector used for all St1Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to V1 sgRNA architecture from Carter et al. biorxiv 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    St1Cas9 sgRNA entry vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    V1 sgRNA architecture from Carter et al. biorxiv …
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133791-plasmid-map-sequence-id-268588

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  9. pUC19-U6-BsmBI_cassette-St3Cas9-sgRNA (KAC27)

    Plasmid
    #133792
    Purpose
    U6 promoter sgRNA entry vector used for all St3Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Muller et al. Molecular Therapy 2015
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    St3Cas9 sgRNA entry vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Muller et al. Molecular T…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133792-plasmid-map-sequence-id-268589

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  11. MoClo adapted pUC57-Kan Level 2

    Plasmid
    #109218
    Purpose
    Non binary level 2 vector adapted for MoClo modular cloning useful for particle bombardment transformation of plants
    Depositor
    Elena Caro Bernat
    Type
    Empty backbone
    Use
    Synthetic Biology
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Nov. 16, 2018
    Availability
    Academic Institutions and Nonprofits only
    109218-plasmid-map-sequence-id-223588

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  12. pUC57 DYSF, alt exon 1, 40a

    Plasmid
    #87894
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1, 40a (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    April 28, 2017
    Availability
    Academic Institutions and Nonprofits only
    87894-plasmid-map-sequence-id-174239

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  13. pUC57 DYSF, alt exon 1, 5a, 17, 40a

    Plasmid
    #87895
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1, 5a, 17, 40a (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    April 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    87895-plasmid-map-sequence-id-176844

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  14. pUC57 DYSF, alt exon 1a, 40a

    Plasmid
    #87901
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1a, 40a (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87901-plasmid-map-sequence-id-174256

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  15. pUC57 DYSF, alt exon 1a, 17, 40a

    Plasmid
    #87899
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1a, 17, 40a (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    March 22, 2017
    Availability
    Academic Institutions and Nonprofits only
    87899-plasmid-map-sequence-id-174253

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  16. pUC57 DYSF, alt exon 1, 17, 40a

    Plasmid
    #87891
    Purpose
    research of alternative isoforms of the protein dysferlin
    Depositor
    Jain Foundation
    Insert
    DYSF, alt exon 1, 17, 40a (DYSF Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    alternative isoforms, see comments
    Promoter
    Available since
    March 21, 2017
    Availability
    Academic Institutions and Nonprofits only
    87891-plasmid-map-sequence-id-174220

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  23. pUC19-U6-BsmBI_cassette-Nme/Nme2Cas9-sgRNA (KAC32)

    Plasmid
    #133794
    Purpose
    U6 promoter sgRNA entry vector used for all NmeCas9 or Nme2Cas9 sgRNAs (clone spacer oligos into BsmBI cassette) - similar to sgRNA architecture from Amrani et al. Genome Biology 2018
    Depositor
    Joseph Bondy-Denomy, Benjamin Kleinstiver
    Article
    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do
    Insert
    NmeCas9 and Nme2Cas9 sgRNA entry vector
    Use
    Tags
    Expression
    Mammalian
    Mutation
    sgRNA architecture from Amrani et al. Genome Biol…
    Promoter
    U6
    Available since
    March 30, 2020
    Availability
    Academic Institutions and Nonprofits only
    133794-plasmid-map-sequence-id-268076

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  26. pUC19_T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP

    Plasmid
    #195503
    Purpose
    SOX17 donor plasmid to generate locus specific C-terminal integration of H2B-mCitrine into the human SOX17-locus
    Depositor
    Alexander Meissner
    Article
    Landshammer et al Elife. 2023 Jan 31;12:e83077. doi: 10.75
    Insert
    SOX17(Ex2)-T2A-H2B-mCitrine_loxP-hPGK-BSD-loxP_SOX17(3'UTR) (SOX17 Human)
    Use
    CRISPR; Donor vector
    Tags
    T2A-H2B-mCitrine
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Feb. 21, 2023
    Availability
    Academic Institutions and Nonprofits only
    195503-plasmid-map-sequence-id-384989

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  34. Padh1-OsTIR1(WT)-NLS-TADH1-Sp.arg3+/pUC19*

    Plasmid
    #169352
    Purpose
    Expression of OsTIR1(WT) in S. pombe from adh1 promoter, integrated at arg3-D4 locus
    Depositor
    Tony Carr
    Insert
    OsTIR1(WT)
    Use
    Tags
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    May 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    169352-plasmid-map-sequence-id-332958

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  40. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1a_(CJT90)

    Plasmid
    #226989
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1a must be used with gRNA 2a or 2b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 1a to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226989-plasmid-map-sequence-id-453964

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  41. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2a_(CJT91)

    Plasmid
    #226991
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2a must be used with gRNA 1a or 1b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 2a to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 21, 2024
    Availability
    Academic Institutions and Nonprofits only
    226991-plasmid-map-sequence-id-453954

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  44. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA2b_(CJT93)

    Plasmid
    #226992
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 2b must be used with gRNA 1a or 1b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 2b to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    226992-plasmid-map-sequence-id-451253

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  45. pUC19-U6-SpCas9_gRNA-OPA1-del_exon5-gRNA1b_(CJT92)

    Plasmid
    #226990
    Purpose
    pUC19 plasmid with U6 promoter and SpCas9 gRNA to create an OPA1-del_exon5 cell line via nuclease mediate excision, gRNA 1b must be used with gRNA 2a or 2b
    Depositor
    Benjamin Kleinstiver
    Article
    Fry et al EMBO J. 2024 Feb;43(3):391-413. doi: 10.
    Insert
    SpCas9 gRNA 1b to create OPA1-del_exon5
    Use
    CRISPR
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    U6
    Available since
    Nov. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    226990-plasmid-map-sequence-id-451250

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  50. pUC19-HDRT-TRAC-CD19.CAR-Cas12a.PAM.mutated

    Plasmid
    #215769
    Purpose
    encodes for HDR template for optimized AsCas12a-mediated knock-in of a CD19-specific CAR into the TRAC locus
    Depositor
    Dimitrios Wagner
    Article
    Glaser et al Genome Biol. 2023 Apr 24;24(1):89. doi:
    Inserts
    TRAC-5' homology arm (TRAC Human)
    P2A-CD19.28.zeta-bgH (CD19 Human, Mouse, Bovine)
    TRAC-3' homology arm (TRAC Human)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Cas12a PAM mutated
    Promoter
    Available since
    June 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    215769-plasmid-map-sequence-id-435524

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Showing: 901 - 950 of 4900 results