We narrowed to 747 results for: PI

1. pAT-9208

   Plasmid

   #124221

   Purpose

   Cloning plasmid for a self-targeting gRNA library

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   self-targeting gRNA
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   J23119

   Available since

   Dec. 18, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. HeFm2SpCas9

   Plasmid

   #92111

   Purpose

   Expression plasmid for human codon-optimized increased fidelity HeFm2SpCas9 (without U6-sgRNA coding sequence)

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

   Insert

   “Highly enhanced Fidelity” mut2 SpCas9
   

   Use

   CRISPR

   Tags

   3xFLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, K848A, Q926A, R1060A

   Promoter

   Cbh

   Available since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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3. Lyn11C3S-EGFP

   Plasmid

   #202747

   Purpose

   weakly plasma membrane targeted fluorescent protein

   Depositor

   Gerry Hammond

   Article

   Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

   Insert

   LYN (1-11) (C3S):EGFP (LYN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pFBD-Akt1(T308A/S473A)

   Plasmid

   #86578

   Purpose

   Expresses human full-length Akt1(T308A/S473A) phosphomutant

   Depositor

   Ivan Yudushkin, Thomas Leonard

   Article

   Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

   Insert

   Akt1 (AKT1 Human)
   

   Use

   Tags

   10xHis

   Expression

   Insect

   Mutation

   T308A/S473A phosphomutant

   Promoter

   Available since

   April 4, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAT15542_nCBE-SuperFi-Cas9

   Plasmid

   #184372

   Purpose

   Mammalian expression of SuperFi-Cas9 CBE base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   nCBE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   3X FLAG, SV40 NLS, and UGI

   Expression

   Mammalian

   Mutation

   D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

   Promoter

   EF-1α core promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAT15544_nABE-SuperFi-Cas9

   Plasmid

   #184374

   Purpose

   Mammalian expression of SuperFi-Cas9 ABE7 base editor

   Depositor

   Ervin Welker

   Article

   Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

   Insert

   nABE-SuperFi-Cas9
   

   Use

   CRISPR

   Tags

   FLAG, SV40 NLS, and nucleoplasmin NLS

   Expression

   Mammalian

   Mutation

   D10A, Y1010D, Y1013D, Y1016D, V1018D, R1019D, Q10…

   Promoter

   EF-1α core promoter

   Available since

   June 1, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAT9750-BEAR-mCherry

   Plasmid

   #162990

   Purpose

   BEAR target plasmid with split mCherry and disrupted 5' splice site

   Depositor

   Ervin Welker

   Article

   Talas et al Nat Commun. 2021 Nov 3;12(1):6353. doi:

   Insert

   mCherry split with an intron between amino acids 119-120
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAT-9222

   Plasmid

   #124222

   Purpose

   Cloning plasmid for EGxFP assay with self-targeting gRNA-s

   Depositor

   Ervin Welker

   Article

   Talas et al Nucleic Acids Res. 2021 Jan 15. pii: 610

   Insert

   self-targeting gRNA between EGFP halves
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Nov. 23, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLKO.1 Puro shRNA INPP5E KD1

   Plasmid

   #162004

   Purpose

   INPP5E shRNA

   Depositor

   David Bryant

   Article

   Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

   Insert

   INPP5E (INPP5E Canis Lupus)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Jan. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330-Flag-wtSpCas9 (without sgRNA)

    Plasmid

    #92353

    Purpose

    Expression plasmid for human codon-optimized wild-type SpCas9 (without U6-sgRNA coding sequence)

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:

    Insert

    3xFlag-NLS-wild-type SpCas9-NLS
    

    Use

    CRISPR

    Tags

    3xFLAG and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Oct. 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLKO.1 Puro shRNA SHIP1 KD2

    Plasmid

    #162013

    Purpose

    SHIP1 shRNA

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    INPP5D (INPP5D Canis Lupus)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLKO.1 Puro shRNA SHIP1 KD1

    Plasmid

    #162012

    Purpose

    SHIP1 shRNA

    Depositor

    David Bryant

    Article

    Roman-Fernandez et al Nat Commun. 2018 Nov 28;9(1):5041. doi:

    Insert

    INPP5D (INPP5D Canis Lupus)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFBD-Akt1(D323A/D325A)

    Plasmid

    #86592

    Purpose

    Expresses human full-length Akt1(D323A/D325A) mutant

    Depositor

    Ivan Yudushkin, Thomas Leonard

    Article

    Ebner et al Mol Cell. 2017 Feb 2;65(3):416-431.e6. d

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Tags

    10xHis

    Expression

    Insect

    Mutation

    D323A/D325A transforming mutations

    Promoter

    Available since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)mGAT1-0-CFP

    Plasmid

    #41664

    Depositor

    Henry Lester

    Article

    Moss et al J Gen Physiol. 2009 Dec;134(6):489-521.

    Insert

    Mus musculus GABA transporter 1 (Slc6a1 Mouse, Synthetic)
    

    Use

    Tags

    CFP

    Expression

    Mammalian

    Mutation

    “Monomerizing” CFP A206K mutation; C-terminal hyd…

    Promoter

    CMV

    Available since

    April 27, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pUC18 HA PTPL1

    Plasmid

    #237335

    Purpose

    expression in bacterial cells for protein purification

    Depositor

    Michele Pagano

    Article

    Kuchay et al Nat Cell Biol. 2013 May;15(5):472-80. do

    Insert

    PTPL1 (PTPN13 Human)
    

    Use

    Tags

    HA

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFNC-5

    Plasmid

    #233297

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Inserts

    BxbI phage integrase
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEM7 and unknown

    Available since

    April 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFNC-6

    Plasmid

    #227669

    Purpose

    Plasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. GmR, easily curable via sucrose counterselection.

    Depositor

    Pablo Ivan Nikel

    Article

    Federici et al Metab Eng. 2025 Jan 6;88:180-195. doi: 1

    Inserts

    BxbI phage integrase
    sacB
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    PEM7

    Available since

    Jan. 22, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pAT15541_dCBE-SuperFi-Cas9

    Plasmid

    #184371

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 CBE base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dCBE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    3X FLAG, SV40 NLS, and UGI

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAT15543_dABE-SuperFi-Cas9

    Plasmid

    #184373

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE7 base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    FLAG, SV40 NLS, and nucleoplasmin NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pAT15545_dABE8e-SuperFi-Cas9

    Plasmid

    #184375

    Purpose

    Mammalian expression of nuclease inactive SuperFi-Cas9 ABE8e base editor

    Depositor

    Ervin Welker

    Article

    Kulcsar et al Nat Commun. 2022 Nov 11;13(1):6858. doi:

    Insert

    dABE8e-SuperFi-Cas9
    

    Use

    CRISPR

    Tags

    Nucleoplasmin NLS and SV40 NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A, Y1010D, Y1013D, Y1016D, V1018D, R101…

    Promoter

    EF-1α core promoter

    Available since

    June 1, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge