We narrowed to 895 results for: THY-2

1. DNMT2Δ191-237

   Plasmid

   #198382

   Purpose

   Bacterial expression plasmid for human DNMT2 deletion mutant; for binding assays

   Depositor

   Tanja Schirmeister

   Article

   Schwickert et al ACS Med Chem Lett. 2023 May 9;14(6):777-

   Insert

   DNMT2 (TRDMT1 Human)
   

   Use

   Tags

   His-tag

   Expression

   Bacterial

   Mutation

   deleted amino acids 191-237

   Promoter

   T7

   Available since

   May 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. SL536

   Plasmid

   #49942

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Dec. 20, 2013

   Availability

   Academic Institutions and Nonprofits only

3. SL539

   Plasmid

   #49948

   Purpose

   Expresses a TALE-TET1CD (catalytic domain of TET1) fusion protein engineered to bind a site in the human RHOXF2/2B homeobox (RHOXF2) gene and demethylate CpGs adjacent to the binding site

   Depositor

   Keith Joung

   Article

   Maeder et al Nat Biotechnol. 2013 Oct 9. doi: 10.1038

   Insert

   RHOXF2 Rhox homeobox family member 2 (RHOXF2 Human)
   

   Use

   TALEN

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 21, 2014

   Availability

   Academic Institutions and Nonprofits only

4. ECON

   Plasmid

   #82553

   Purpose

   Epigenetic effector construct for targeted methylation

   Depositor

   Joseph Irudayaraj

   Article

   Choudhury et al Oncotarget. 2016 Jul 4. doi: 10.18632/on

   Inserts

   DNMT3A (DNMT3A Human)
   CRY2 (CRY2 Mustard Weed)
   

   Use

   Tags

   HALO and mCherry

   Expression

   Mammalian

   Mutation

   N-terminal photolyase homology region domain of C…

   Promoter

   CMV

   Available since

   Sept. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-6xHis-MBP-TEV site-TadA8.20

   Plasmid

   #194702

   Purpose

   Expresses N-terminal MBP fused TadA8.20 in bacteria

   Depositor

   Weixin Tang

   Article

   Xiao et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

   Insert

   TadA8.20
   

   Use

   Tags

   6xHis and MBP

   Expression

   Bacterial

   Mutation

   mutations in TadA are described in the paper

   Promoter

   T7 promoter

   Available since

   Feb. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. MSCB-hDot1Lmut

   Plasmid

   #74174

   Purpose

   For packaging hDot1Lmut (catalytic activity dead) to retrovirus.

   Depositor

   Yi Zhang

   Article

   Okada et al Cell. 2005 Apr 22;121(2):167-78.

   Insert

   hDot1L (DOT1L Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mutation

   hDot1L 163 GSG to RCR mutation*

   Promoter

   Available since

   Aug. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

7. pTK305

   Plasmid

   #66812

   Purpose

   IVT vector for Fluc mRNA

   Depositor

   Ron Weiss

   Article

   Andries et al J Control Release. 2015 Sep 2. pii: S016

   Insert

   Firefly luciferase
   

   Use

   Luciferase; Mrna ivt

   Tags

   Expression

   Mutation

   Promoter

   T7

   Available since

   Sept. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAtipiTrc

   Plasmid

   #53275

   Purpose

   Contains an Arabidopsis thaliana idi1 cDNA fused to a strong Trc promoter. Produces an active isopentenyl diphosphate isomerase (IDI) in E. coli.

   Depositor

   Francis X Cunningham Jr

   Article

   Cunningham FX et al J Bacteriol. 2000 Oct;182(20):5841-8.

   Insert

   ipi1 (IPP2 Mustard Weed)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Trc

   Available since

   Jan. 21, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. CRY2(delta2-6)-mCherry

   Plasmid

   #104182

   Purpose

   Expresses fusion of CRY2(delta aa 2-6) with mCherry

   Depositor

   Bianxiao Cui

   Article

   Duan et al Nat Commun. 2017 Sep 15;8(1):547. doi: 1

   Insert

   CRY2(delta2-6)-mCherry
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   CRY2PHR with deletion of aa 2-6

   Promoter

   CMV

   Available since

   Dec. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pET28a-YJR129c

    Plasmid

    #85118

    Purpose

    bacterial expression of YJR129c

    Depositor

    Pal Falnes

    Article

    Davydova et al J Biol Chem. 2014 Oct 31;289(44):30499-5

    Insert

    EFM3 (EFM3 Budding Yeast)
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. dCas9-10xSunTag-P2A-tagBFP

    Plasmid

    #174141

    Purpose

    Expresses the dCas9-10xSunTag-tagBFP fusion protein for the recruitment of scFv(GCN4)-fused effector domains. A shorter version of the Addgene 60903 plasmid.

    Depositor

    Albert Jeltsch

    Article

    Hofacker et al Int J Mol Sci. 2020 Jan 13;21(2). pii: i

    Insert

    dCas9, 10xSunTag, tagBFP
    

    Use

    Tags

    HA and tagBFP

    Expression

    Mammalian

    Mutation

    Promoter

    SV40

    Available since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX4T-1 Srap1

    Plasmid

    #89691

    Purpose

    Expresses murine Srap1 in bacteria

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    Murine SRAP domain ortholog Srap1 (Hmces Mouse)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    May 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET30a-SRAP

    Plasmid

    #84524

    Purpose

    Expresses murine SRAP domain (250 aa) in bacteria

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    SRAP domain (Hmces Mouse)
    

    Use

    Tags

    6xHis and S-Tag

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AV4-EF1-GFP

    Plasmid

    #124971

    Purpose

    Control for AV4-EF1-NMNAT2

    Depositor

    Kazuhiro Oka

    Article

    Ljungberg et al Hum Mol Genet. 2012 Jan 15;21(2):251-67.

    Insert

    GFP
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1-alpha

    Available since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. Banshee-ShCit-A

    Plasmid

    #85216

    Purpose

    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

    Depositor

    Alex Minella

    Article

    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

    Insert

    shCit-A
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Banshee-ShCit-B

    Plasmid

    #85217

    Purpose

    Short hairpin RNAs were designed to target murine Cit (encoding Citron Rho-interacting kinase) and were subcloned into the Banshee-GFP vector for expression under control of the H1 promoter.

    Depositor

    Alex Minella

    Article

    Swartz et al Mol Cell Biol. 2016 Oct 17. pii: MCB.002

    Insert

    shCit-B
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    H1

    Available since

    Feb. 3, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET30a-SRAP-delCys2

    Plasmid

    #84568

    Purpose

    Encodes murine SRAP domain (250aa) of Hmces/Srap1 with deletion of catalytic Cys2

    Depositor

    Douglas Feldman

    Article

    Kweon et al Cell Rep. 2017 Oct 10;21(2):482-494. doi

    Insert

    Srap1 (Hmces Mouse)
    

    Use

    Tags

    6xHis and S-Tag

    Expression

    Bacterial

    Mutation

    deletion of Cys2

    Promoter

    T7

    Available since

    Nov. 18, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pJS699

    Plasmid

    #168779

    Purpose

    Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

    Depositor

    Timothy Whitehead

    Article

    Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

    Insert

    Spike RBD (S betacoronavirus)
    

    Use

    Tags

    Cmyc

    Expression

    Mutation

    N343Q

    Promoter

    GAL1

    Available since

    Aug. 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3/HA-FLAG-RBP2

    Plasmid

    #14800

    Depositor

    William Kaelin

    Article

    Klose et al Cell. 2007 Mar 9. 128(5):889-900.

    Insert

    Retinoblastoma Binding Protein 2 (KDM5A Human)
    

    Use

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    April 14, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCMV-Pam-FL

    Plasmid

    #42570

    Depositor

    Vijaya Ramesh

    Article

    Santos et al J Neurosci Res. 2006 Feb 1;83(2):222-32.

    Insert

    human MYCBP2 (MYCBP2 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Pam-FL clone lacks alternatively spliced exons 52…

    Promoter

    CMV

    Available since

    March 14, 2013

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge