We narrowed to 4,047 results for: bre

1. GW1-mScarlet-Epac-GeNL

   Plasmid

   #207874

   Purpose

   Mammalian expression of the FRET/BRET cAMP sensor

   Depositor

   Mathew Tantama

   Article

   French et al ACS Omega. 2019 Sep 12;4(13):15504-15511

   Insert

   mScarlet-hEpac1(149-881)-mNeonGreen-NanoLuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. PB_rtTA_BsmBI

   Plasmid

   #126028

   Purpose

   PiggyBac cargo vector expressing rtTA, contains BsmBI sites for sgRNA cloning

   Depositor

   Mauro Calabrese

   Article

   Schertzer et al RNA. 2019 May 17. pii: rna.068932.118. d

   Type

   Empty backbone

   Use

   CRISPR; Piggybac

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   June 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. mNeonGreen-DEVD-NanoLuc

   Plasmid

   #98287

   Purpose

   caspase-3 BRET Sensor

   Depositor

   Maarten Merkx

   Article

   den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

   Insert

   mNeonGreen-LEHD-NanoLuc
   

   Use

   Tags

   His-tag

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 11, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pMB34

   Plasmid

   #122245

   Purpose

   Lentivirus delivery for stable expression of LbCas12a-VPR

   Depositor

   Darjus Tschaharganeh

   Article

   Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

   Insert

   mammalian codon-optimized Lachnospiraceae bacterium Cas12a fused to VPR activator
   

   Use

   Lentiviral

   Tags

   NLS and VPR activator

   Expression

   Mutation

   Promoter

   EFs

   Available since

   March 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. STAGR_gRNAScaffold_hU6

   Plasmid

   #102840

   Purpose

   Can be used as PCR template for a STAgR reaction

   Depositor

   Stefan Stricker

   Article

   Breunig et al PLoS One. 2018 Apr 27;13(4):e0196015. do

   Insert

   gRNAScaffold_hU6promoter
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   hU6

   Available since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pcDNA5-FRT-TO-N-Flag-8His

   Plasmid

   #157677

   Purpose

   N-terminal tagging vector

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   Flag-8His
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 4, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

7. pLenti_CMV: Flag-Antares_SV40:PuroR

   Plasmid

   #183049

   Purpose

   Expresses FLAG-tagged Antares in mammalian cells.

   Depositor

   Antonio Amelio

   Article

   Yao et al Nat Methods. 2022 Jul;19(7):893-898. doi

   Insert

   Antares BRET reporter
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT-TO-eGFP

   Plasmid

   #157733

   Purpose

   mammalian expression of untagged eGFP under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   eGFP
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pLV hUbC-Cas9-P2A-Puro_BsmBI-sgRNA-BsmBI

   Plasmid

   #188703

   Purpose

   Lentivirus transfer plasmid encoding Cas9, puromycin resistance, and a BsmBI array for cloning 4 sgRNAs

   Depositor

   David Tobin

   Article

   Brewer et al Cell Rep. 2022 Dec 13;41(11):111817. doi

   Type

   Empty backbone

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. mNeonGreen-IETD-NanoLuc

    Plasmid

    #98288

    Purpose

    caspase-8 BRET Sensor

    Depositor

    Maarten Merkx

    Article

    den Hamer et al ACS Sens. 2017 Jun 23;2(6):729-734. doi:

    Insert

    mNeonGreen-LEHD-NanoLuc
    

    Use

    Tags

    His-tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 11, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pMC_mClover3_BSDminus_F0

    Plasmid

    #184165

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 0, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pGEX6P1-UBE2S-UBD

    Plasmid

    #66713

    Purpose

    Expresses the UBE2S-UBD fusion protein in E. coli

    Depositor

    David Komander

    Article

    Bremm et al Nat Struct Mol Biol. 2010 Aug;17(8):939-

    Inserts

    e2-epf (UBE2S )
    isot (USP5 )
    

    Use

    Tags

    GST-3C

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pcDNA5-FRT-TO-C-8His-Flag

    Plasmid

    #157678

    Purpose

    C-terminal tagging vector

    Depositor

    David Tollervey

    Article

    Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

    Insert

    8His-Flag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pMC_mClover3_BSDminus_F2

    Plasmid

    #184167

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 2, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pMC_mClover3_BSDminus_F1

    Plasmid

    #184166

    Purpose

    Donor cassette plasmid for high-throughput tagging, encoding an mClover3 synthetic exon in frame 1, as well as a blasticidin resistance gene for selection of genomic integrants.

    Depositor

    Ophir Shalem

    Article

    Serebrenik et al Genome Res. 2019 Aug;29(8):1322-1328. do

    Inserts

    mClover3
    bsd
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. SeeSaw Reporter (SSR1.0)

    Plasmid

    #50392

    Purpose

    SSR1.0 has a one I-SceI target site for generate specific double strand break after I-SceI endonuclease expression.

    Depositor

    Pablo Huertas Sánchez

    Article

    Gomez-Cabello et al PLoS One. 2013 Oct 14;8(10):e77206. doi:

    Insert

    SSR1.0
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 14, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pMB22

    Plasmid

    #122244

    Purpose

    Lentivirus delivery for stable expression of AsCas12a-VPR

    Depositor

    Darjus Tschaharganeh

    Article

    Breinig et al Nat Methods. 2019 Jan;16(1):51-54. doi:

    Insert

    mammalian codon-optimized Acidaminococcus sp. Cas12a fused to VPR activator
    

    Use

    Lentiviral

    Tags

    NLS and VPR activator

    Expression

    Mutation

    Promoter

    EFs

    Available since

    May 20, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. RCAS(A) mPea3 FL (CT#1119)

    Plasmid

    #13908

    Depositor

    Cliff Tabin

    Article

    Brent et al Development. 2004 Aug . 131(16):3885-96.

    Insert

    Pea3 (Etv4 Mouse)
    

    Use

    Retroviral; Avian expression

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Feb. 16, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pTAJAK-71 (pESC-NatMXsyn-USER)

    Plasmid

    #78232

    Purpose

    Empty vector, for the insertion of gRNA expression cassettes into the vector.

    Depositor

    Irina Borodina

    Article

    Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Nov. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-RaACR_687-EYFP

    Plasmid

    #111077

    Purpose

    Anion channelrhodopsin from Rhodomonas abbreviata for expression in mammalian cells

    Depositor

    John Spudich

    Article

    Govorunova et al eNeuro. 2018 Jul 10;5(3). pii: eN-MNT-01

    Insert

    Anion channelrhodopsin RaACR_687
    

    Use

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 4, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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