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Addgene
Showing: 361 - 380 of 16401 results
  1. pZF-BCB-GCN

    Plasmid
    #127476
    Purpose
    coding sequence for synthetic zinc finger ZF-BCB-GCN
    Depositor
    Insert
    ZF-BCB synthetic transcription factor fused with GCN4 leucine zipper
    Use
    Cell-free transcription–translation
    Tags
    GCN4 leucine zipper
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 2, 2019
    Availability
    Academic Institutions and Nonprofits only
  2. pZF-BCB-PDZ

    Plasmid
    #127478
    Purpose
    coding sequence for synthetic zinc finger ZF-BCB-PDZ
    Depositor
    Insert
    ZF-BCB synthetic transcription factor fused with the alpha 1-syntrophin PDZ domain
    Use
    Cell-free transcription–translation
    Tags
    alpha 1-syntrophin PDZ domain
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 8, 2019
    Availability
    Academic Institutions and Nonprofits only
  3. pZF-ADD-L

    Plasmid
    #127477
    Purpose
    coding sequence for synthetic zinc finger ZF-ADD-L
    Depositor
    Insert
    ZF-ADD synthetic transcription factor fused with PDZ cognate peptide ligand (VKESLV)
    Use
    Cell-free transcription–translation
    Tags
    PDZ cognate peptide ligand (VKESLV)
    Expression
    Mutation
    Promoter
    T7
    Available since
    Nov. 15, 2019
    Availability
    Academic Institutions and Nonprofits only
  4. pZF-ADD-GCN

    Plasmid
    #127475
    Purpose
    coding sequence for synthetic zinc finger ZF-ADD-GCN
    Depositor
    Insert
    ZF-ADD synthetic transcription factor fused with GCN4 leucine zipper
    Use
    Cell-free transcription–translation
    Tags
    GCN4 leucine zipper
    Expression
    Mutation
    Promoter
    T7
    Available since
    July 9, 2019
    Availability
    Academic Institutions and Nonprofits only
  5. mAIRN HO

    Plasmid
    #207411
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
  6. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Insert
    mAIRN
    Use
    Tags
    Expression
    Bacterial and Mammalian
    Mutation
    Promoter
    Available since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
  7. dSpCas9-VPR

    Plasmid
    #205247
    Purpose
    In vitro transcription plasmid for production of dSpCas9-VPR mRNA for transcriptional activation (VPR = tripartite transactivator VP64, p65, and Rta). Insert is derived from Addgene plasmid #63798.
    Depositor
    Insert
    dSpCas9-VPR
    Use
    CRISPR
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
  8. pHrD-IRES-Fluc

    Plasmid
    #194250
    Purpose
    Human RNAP1 promoter region driving firefly luciferase expression from IRES. Constructed by Ghoshal, et al. J Biol Chem (2004). Please cite this original manuscript!
    Depositor
    Insert
    Firefly luciferase
    Use
    Luciferase
    Tags
    Expression
    Mutation
    Promoter
    human rDNA promoter and beginning of 5' ETS …
    Available since
    April 27, 2023
    Availability
    Academic Institutions and Nonprofits only
  9. dRT-pMDLg/pRRE

    Plasmid
    #60488
    Purpose
    gag/pol packaging plasmid for production of lentiviral vectors with mutated reverse transcriptase (NRTLV, non-reverse transcribable lentiviral vectors)
    Depositor
    Insert
    HIV-1 gag, HIV-1 pol with inactive reverse transcriptase
    Use
    Tags
    Expression
    Mutation
    changed Asp at position 249 + 250 to Val
    Promoter
    Available since
    Oct. 16, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 361 - 380 of 16401 results