We narrowed to 2,323 results for: hells

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CMV-hCas9-T2A-mStrawberry

Plasmid

#187224

Purpose

Expresses human Cas9 nuclease and mStrawberry via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Use

CRISPR

Tags

Inserted T2A-mStrawberry at 3' terminal of M…

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10RFP-Pct5.1-crRNA(AarI)-RFP

Plasmid

#192273

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(AarI)-RFP, entry plasmid for crRNA cloning

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.9RT2-Pct5.1-crRNA(cadR)-RT(ΔcadR)

Plasmid

#191647

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cadR-targeting spacer) cadR-deleting repair template,used for cadR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.1RT1-Pct5.1-crRNA(dadR)-RT(ΔdadR)

Plasmid

#191635

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(dadR-targeting spacer) dadR-deleting repair template, used for dadR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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CMV-hCas9-T2A-miRFP670

Plasmid

#187223

Purpose

Expresses human Cas9 nuclease and miRFP670 via T2A linker under control of a CMV promoter.

Depositor

Jennifer Mitchell

Article

Abatti et al Nucleic Acids Res. 2023 Sep 22:gkad734.

Insert

Cas9

Tags

Inserted T2A-miRFP670 at 3' terminal of MCS.

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-VTKS5-TVA-eGFP-N2cG

Plasmid

#175440

Purpose

Cre-on / Flp-off helpers for N2c rabies monosynaptic tracing; TVA, eGFP and N2cG under Synpasin promotoer

Depositor

Gordon Fishell

Article

Pouchelon et al Cell Rep. 2021 Nov 9;37(6):109993. doi:

Insert

TVA-P2A-eGFP-P2A-N2cG

Use

AAV and Cre/Lox

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Nanog-t2A-mCherry

Plasmid

#127541

Purpose

Expresses the transactivation domain of mouse Nanog and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Nanog (Nanog Mouse)

Expression

Mammalian

Mutation

Codes for the last 110 a.a of Nanog

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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CD200CD4d3+4-His

Plasmid

#36152

Purpose

A positive control receptor bait with His-tag (rat CD4d3+4-HIS) and prey (rat CD200) EXPRESs plasmid

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 (Cd200 Rat)

Tags

His tag and ratCD4d3+4

Expression

Mammalian

Mutation

G2A

Promoter

CMV

Available Since

May 14, 2012

Availability

Academic Institutions and Nonprofits only

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GIL-6

Plasmid

#213389

Purpose

Expresses a chimeric cytokine between IL-6 and LIF

Depositor

Jurgen Scheller

Article

Rafii et al J Biol Chem. 2024 May;300(5):107251. doi

Insert

GIL-6

Tags

IgG1-Fc-tag and Myc-tg

Expression

Mammalian

Promoter

T7

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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GIO-6

Plasmid

#213390

Purpose

Expresses a chimeric cytokine between IL-6 and OSM

Depositor

Jurgen Scheller

Article

Rafii et al J Biol Chem. 2024 May;300(5):107251. doi

Insert

GIO-6

Tags

IgG1-Fc-tag and Myc-tg

Expression

Mammalian

Promoter

T7

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.6RT4-Pct5.1-crRNA(hcdR)-RT(ΔhcdR)

Plasmid

#191646

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(hcdR-targeting spacer) hcdR-deleting repair template, used for hcdR deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1/cyto-iAspSnFR

Plasmid

#201394

Purpose

CMV overexpression of cyto-iAspSnFR in mammalian cell lines

Depositor

Kai Johnsson

Article

Hellweg et al Cell Chem Biol. 2024 May 20:S2451-9456(2

Insert

cyto-iAspSnFR

Expression

Mammalian

Mutation

gltI5-253: Y42F, S72A, T92D, G111T, K192R, Y211W,…

Promoter

CMV

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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CA-Stat3-t2A-mCherry

Plasmid

#127540

Purpose

Expresses constitutively active (CA) mouse Stat3 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

Stat3 (Stat3 Mouse)

Expression

Mammalian

Mutation

A662C and N664C. Residues changed to Cys renders …

Promoter

CMV

Available Since

July 25, 2019

Availability

Academic Institutions and Nonprofits only

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pmiRGLO Dual-Luciferase-PDAP1-3'UTR-Mut4x

Plasmid

#182256

Purpose

Human PDAP1 3` UTR region mutated in 4x binding site for miR-150 cloned downstream of luciferase reporter gene

Depositor

Silvia Monticelli

Article

Chirichella et al PLoS Biol. 2022 Feb 10;20(2):e3001538. d

Insert

3' UTR region of PDAP1 gene mutated in 4 binding sites for mir-150

Use

Luciferase

Expression

Mammalian

Available Since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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Sox2-t2A-GFP

Plasmid

#127537

Purpose

Expresses human SOX2 and eGFP via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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CD200-Cd4d3+4-COMP-blac-3xFLAG-6his

Plasmid

#71471

Purpose

A positive control receptor prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit

Depositor

Gavin Wright

Article

Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

Insert

CD200 (Cd200 Rat)

Tags

6xHis tag, Cd4d3+4, beta-lactamase, and triple FL…

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2016

Availability

Academic Institutions and Nonprofits only

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Sox2-t2A-mCherry

Plasmid

#127538

Purpose

Expresses human SOX2 and mCherry via t2A linker.

Depositor

Jennifer Mitchell

Article

Dhaliwal et al Genes Dev. 2019 Jun 20. pii: gad.324319.

Insert

SOX2 (SOX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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MoFGFR3b-SV40PA

Plasmid

#86493

Purpose

mamalian cell expression of FGFR3b

Depositor

David Ornitz

Article

Chellaiah et al J Biol Chem. 1994 Apr 15;269(15):11620-7

Insert

FgfrR3 IIIb 12F5 (Fgfr3 Mouse)

Expression

Mammalian

Promoter

Moloney murine leukemia virus LTR

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pXD71Cas10.4RT4-Pct5.1-crRNA(cgr2)-RT(Δcgr12)

Plasmid

#191650

Purpose

E. coli - Eggerthella lenta shuttle plasmid (KanR), cumate-inducible promoter Pct5.1-crRNA(cgr2-targeting spacer) cgr1/cgr2-deleting repair template, used for cgr1/cgr2 deletion

Depositor

Emily Balskus

Article

Dong et al Nat Commun. 2022 Dec 9;13(1):7624. doi:

Insert

CymR

Use

CRISPR

Expression

Bacterial

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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gZiPro

Plasmid

#198441

Purpose

Activity/inhibition assays, 19F-NMR

Depositor

Ute Hellmich, Tanja Schirmeister

Article

Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

Insert

glycine linked Zika virus NS2B-NS3

Tags

His6-TEV

Expression

Bacterial

Mutation

R95*A,

Promoter

T7 promoter

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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