159,557 results

1. pcDNA5FRT/Flag-FLJ10081

   Plasmid

   #64775

   Purpose

   Mammalian expression of Flag-tagged FLJ10081 (NSL3)

   Depositor

   Joan Conaway, Ronald Conaway

   Article

   Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

   Insert

   FLJ10081 (KANSL3 Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 19, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEF Synaptophysin-mRuby

   Plasmid

   #188980

   Purpose

   Synaptic buton marker

   Depositor

   Edwin Chapman

   Article

   Courtney et al Neuron. 2018 Jun 6;98(5):977-991.e5. doi

   Insert

   Synaptophysin (Syp Rat, Synthetic)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 21, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pBS-ldhGFP

   Plasmid

   #27170

   Depositor

   Michela Lizier

   Article

   Lizier et al FEMS Microbiol Lett. 2010 Jul 1. 308(1):

   Insert

   lactate dehydrogenase promoter region fused with modified green fluorescent protein 5
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 24, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. C1-F-tractin-mCherry

   Plasmid

   #155218

   Purpose

   Monitor global F-actin distribution

   Depositor

   Tobias Meyer

   Article

   Bisaria et al Science. 2020 Jun 12;368(6496):1205-1210

   Insert

   F-tractin (aa 9-52 of rat ITPKA) fused to mCherry (Itpka Rat)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCW57-GFP-2A-MCS

   Plasmid

   #71783

   Purpose

   All-in-one doxycycline inducible lentiviral vector for expression of one gene in combination with turbo GFP using the P2A self-cleaving peptide.

   Depositor

   Adam Karpf

   Article

   Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

   Has Service

   Cloning Grade DNA

   Type

   Empty backbone

   Use

   Lentiviral; Doxycycline inducible; p2a self cleav…

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pACM4

   Plasmid

   #49797

   Purpose

   ePathBrick vector for pathway engineering in E. coli.

   Depositor

   Mattheos Koffas

   Article

   Xu et al ACS Synth Biol. 2012 Jul 20;1(7):256-66.

   Type

   Empty backbone

   Use

   Tags

   S-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Jan. 31, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAX APP 695

   Plasmid

   #30137

   Depositor

   Tracy Young-Pearse, Dennis Selkoe

   Article

   Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

   Insert

   Amyloid Precursor Protein (APP Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   wild type splice variant 695

   Promoter

   Available since

   Aug. 15, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-EGFP-Cdc42-T17N

   Plasmid

   #12976

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Cdc42 DN (CDC42 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Promoter

   CMV

   Available since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pNP1830

   Plasmid

   #222728

   Purpose

   pNP561 - T5 Target with T7 Inducible IS621 Recombinase

   Depositor

   Patrick Hsu

   Article

   Durrant et al Nature. 2024 Jun;630(8018):984-993. doi:

   Insert

   IS621 Recombinase
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   July 24, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pGWB451

    Plasmid

    #74833

    Purpose

    Gateway cloning compatible binary vector for C-terminal fusion with G3GFP (CaMV35S promoter).

    Depositor

    Tsuyoshi Nakagawa

    Article

    Nakagawa et al Biosci Biotechnol Biochem. 2007 Aug;71(8

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    CaMV35S

    Available since

    July 20, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28:GFP

    Plasmid

    #60733

    Purpose

    pET28 driving expression of GFP, biobrick part Bba_E0040

    Depositor

    Matthew Bennett

    Article

    Shis et al Proc Natl Acad Sci U S A. 2013 Mar 26;11

    Insert

    GFP
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Nov. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pMo130

    Plasmid

    #27388

    Purpose

    Burkholderia allelic exchange suicide vector pMo130, ref EU862243

    Depositor

    Martin Voskuil

    Article

    Hamad et al Gene. 2009 Feb 1. 430(1-2):123-31.

    Type

    Empty backbone

    Use

    Burkholderia allelic exchange suicide vector pmo1…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    March 7, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA5/FRT/TO_HaloTag9_LamB1

    Plasmid

    #175538

    Purpose

    Expression of HaloTag9 at the nuclear lamina of the mammalian cells

    Depositor

    Kai Johnsson

    Article

    Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

    Insert

    HaloTag9-LamB1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    HaloTag9 = HaloTag7-Q165H-P174R

    Promoter

    Available since

    Dec. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pgRNA-humanized

    Plasmid

    #44248

    Purpose

    Expression of customizable guide RNA (gRNA) under cotnrol of murine U6 promoter, also contains a CMV-puro-t2A-mCherry expression cassette for mammalian gene knockdown

    Depositor

    Stanley Qi

    Article

    Qi et al Cell. 2013 Feb 28;152(5):1173-83. doi: 1

    Insert

    guide RNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mouse U6

    Available since

    April 8, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. iF66 PB-LoxStopLox-Zim3-dCas9-mScarlet-hygro

    Plasmid

    #204731

    Purpose

    Cre-inducible CRISPRi by Zim3-dCas9

    Depositor

    Michael Ward, iPSC Neurodegenerative Disease Initiative (iNDI)

    Insert

    Zim3-dCas9
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pKRaION2-N1

    Plasmid

    #177802

    Purpose

    Mammalian expression of green fluorescent potassium indicator KRaION2

    Depositor

    Edward Boyden

    Article

    Torres Cabán et al ACS Sens. 2022 May 27;7(5):1336-1346. do

    Insert

    KRaION2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. Flag-HA-USP30

    Plasmid

    #22578

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP30 (USP30 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Promoter

    Available since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. G protein alpha-s-GFP

    Plasmid

    #66083

    Purpose

    G protein alpha-s internally tagged with EGFP and EE epitope

    Depositor

    Catherine Berlot

    Article

    Hynes et al J Biol Chem. 2004 Oct 15;279(42):44101-1

    Insert

    G-alpha-s-EE-EGFP (Gnas Rat)
    

    Use

    Tags

    EGFP flanked by SGGGS on each side was inserted i…

    Expression

    Mammalian

    Mutation

    BamHI sites in the alpha-s cDNA were removed and …

    Promoter

    CMV

    Available since

    Aug. 12, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. PX458 V3

    Plasmid

    #226957

    Purpose

    CBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.

    Depositor

    Paul Thomas

    Article

    Chey et al BMC Genomics. 2025 Feb 12;26(1):138. doi

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Oct. 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCLXSN(GFP)-mTRAF2

    Plasmid

    #174736

    Purpose

    Gene overexpression

    Depositor

    Shao-Cong Sun

    Insert

    TRAF2 (Traf2 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    WT

    Promoter

    Available since

    Sept. 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pNF02-mChartreuse

    Plasmid

    #219397

    Purpose

    Constitutive expression of mChartreuse in E. coli

    Depositor

    Christian Lesterlin

    Article

    Fraikin et al Sci Adv. 2025 Apr 18;11(16):eads6201. do

    Insert

    mChartreuse
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    proDp

    Available since

    July 10, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. PLEX-PGK-LAMP1-HaloTag

    Plasmid

    #164216

    Purpose

    pLEX lentivirus backbone expresses HaloTag tagged LAMP1 under PGK promoter

    Depositor

    Jennifer Lippincott-Schwartz

    Article

    Liao et al Cell. 2019 Sep 19;179(1):147-164.e20. do

    Insert

    LAMP1 (Lamp1 Rat)
    

    Use

    Lentiviral

    Tags

    HaloTag

    Expression

    Mutation

    Promoter

    PGK

    Available since

    Jan. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pHAGE-IRES-puro-NLS-dPspCas13b-3xEGFP-NLS-3xFlag

    Plasmid

    #132398

    Purpose

    overexpression in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Mol Cell. 2019 Dec 19;76(6):981-997.e7.

    Insert

    dPspCas13b
    

    Use

    Lentiviral

    Tags

    flag

    Expression

    Mammalian

    Mutation

    H133A; H1059A

    Promoter

    Available since

    Jan. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pX-RCC1-HA

    Plasmid

    #106938

    Purpose

    Transient expression of HA-tagged RCC1 in mammalian cells (CMV promoter)

    Depositor

    Patrizia Lavia

    Article

    DeLuca et al Cancer Res. 2003 Mar 15;63(6):1430-7.

    Insert

    human RCC1-HA (RCC1 Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CMV promoter

    Available since

    March 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCAG-Scarlet-Kif5a

    Plasmid

    #118745

    Purpose

    Expresses scarlet tagged Kif5a

    Depositor

    Beverly Koller

    Insert

    Kif5a cDNA (Kif5a Mouse)
    

    Use

    Tags

    mScarlet

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCDNA4.TO-K5-2xCSTREP

    Plasmid

    #136154

    Purpose

    Expresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5

    Depositor

    Britt Glaunsinger

    Article

    Davis et al Mol Cell. 2015 Jan 22;57(2):349-60. doi:

    Insert

    K5
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. HaloTag-sec61b

    Plasmid

    #186959

    Purpose

    Express sec61b connected with N-terminus HaloTag

    Depositor

    Ke Xu

    Article

    Wang et al Proc Natl Acad Sci U S A. 2022 May 3;119

    Insert

    Sec61 (SEC61B Human)
    

    Use

    Tags

    HaloTag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV Promoter

    Available since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pMDG

    Plasmid

    #187440

    Purpose

    VSV-G envelope expressing plasmid

    Depositor

    Simon Davis

    Article

    Susac et al Cell. 2022 Aug 18;185(17):3201-3213.e19.

    Inserts

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 29, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. secreted BirA-Flag

    Plasmid

    #64395

    Purpose

    Flag-tagged biotinylation enzyme for co-expression with bio-proteins

    Depositor

    Gavin Wright

    Article

    Bushell et al Genome Res. 2008 Apr;18(4):622-30. Epub

    Insert

    BirA (birA E. coli)
    

    Use

    Tags

    Flag tag

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pcDNA5FRT/Flag-LOC339287(MSL1)

    Plasmid

    #29762

    Depositor

    Joan Conaway

    Article

    Cai et al J Biol Chem. 2010 Feb 12. 285(7):4268-72

    Insert

    MSL1 (MSL1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 15, 2013

    Availability

    Academic Institutions and Nonprofits only