We narrowed to 9,187 results for: Pol;

1. pCMV6-AN-DDK_V222A RAD23B

   Plasmid

   #201580

   Purpose

   Expresses a variant of human RAD23B containing mutation V222A within UBA1. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutation V222A

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMV6-AN-DDK_F400A RAD23B

   Plasmid

   #201581

   Purpose

   Expresses a variant of human RAD23B containing mutation F400A within UBA2. Variant of plasmid pCMV6-AN-DDK_WT RAD23B

   Depositor

   Roger Woodgate

   Article

   Ashton et al J Mol Biol. 2023 Nov 5:168353. doi: 10.1

   Insert

   UV excision repair protein RAD23 homolog B (RAD23B Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Contains mutation F400A

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMx/ATP11C-b(E184Q)-HA

   Plasmid

   #209264

   Purpose

   Mammalian expression of ATP11C isoform b mutant E184Q

   Depositor

   Hye-Won Shin

   Article

   Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

   Insert

   ATP11C isoform b (ATP11C Human)
   

   Use

   Retroviral

   Tags

   HA

   Mutation

   E184Q

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG/ATP11C-c-HA

   Plasmid

   #209222

   Purpose

   Mammalian expression of ATP11C isoform c

   Depositor

   Hye-Won Shin

   Article

   Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

   Insert

   ATP11C isoform c (ATP11C Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG/ATP11C-b-HA

   Plasmid

   #209221

   Purpose

   Mammalian expression of ATP11C isoform b

   Depositor

   Hye-Won Shin

   Article

   Inoue et al J Cell Sci. 2021 Oct 15;134(20):jcs25852

   Insert

   ATP11C isoform b (ATP11C Human)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 7, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. A3Bctd-L7G-Cas9n-UGI-NLS

   Plasmid

   #198886

   Purpose

   Express A3Bctd-L7G BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Loop 7 of A3G

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. A3Bctd-D314E-Cas9n-UGI-NLS

   Plasmid

   #198887

   Purpose

   Express A3Bctd-D314E BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3B C-terminal domain (APOBEC3B Human)
   

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   D314E

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. eA3Ai-Cas9n-UGI-NLS

   Plasmid

   #207165

   Purpose

   Express A3A with an N57G point mutation and an intron in a BE3-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
   

   Tags

   Cas9-UGI--NLS

   Expression

   Mammalian

   Mutation

   N57G

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. eA3Ai-max

   Plasmid

   #207166

   Purpose

   Express A3A with an N57G point mutation and an intron in a BE4max-based base editor

   Depositor

   Reuben Harris

   Article

   Rieffer et al CRISPR J. 2023 Oct;6(5):430-446. doi: 10

   Insert

   Apolipoprotein B mRNA Editing Enzyme, Catalytic Polypeptide-like 3A (APOBEC3A Human)
   

   Tags

   Cas9-UGI-UGI-NLS and NLS

   Expression

   Mammalian

   Mutation

   N57G

   Promoter

   CMV

   Available Since

   Nov. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMVQA

    Plasmid

    #197894

    Purpose

    pCR®2.1-TOPO® backbone with 3' end of HIV-1(HXB2:9045 till poly-A (20))

    Depositor

    Thomas Klimkait

    Article

    Otte et al Cell Rep Methods. 2023 May 23;3(6):10048

    Insert

    HIV-1 3' end (HXB2: 9045 till poly-A(10)
    

    Expression

    Bacterial

    Available Since

    June 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-DIO-basic

    Plasmid

    #196882

    Purpose

    Used for preparation of double-floxed inverse ORF vectors

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Type

    Empty backbone

    Use

    Cre/Lox; Dre/rox

    Expression

    Mammalian

    Available Since

    May 23, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pBactin-mOrange2-CI

    Plasmid

    #196852

    Purpose

    Expression of red fluorescent reporter mOrange driven by the strong Beta-actin promoter

    Depositor

    Frank Bradke

    Article

    Vinopal et al Neuron. 2023 Apr 19;111(8):1241-1263.e16

    Insert

    mOrange2
    

    Expression

    Mammalian

    Mutation

    None (wt)

    Promoter

    Chicken bactin (plus Chicken bactin intron)

    Available Since

    May 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)

    Plasmid

    #197969

    Purpose

    Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    L1332V, delta(1616-1666)

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pDARMO_CMVT_3xFLAG_BIRC6_delta(Helix)_BIRmut

    Plasmid

    #197970

    Purpose

    Mammalian cell expression of 3xFLAG-BIRC6_delta(Helix)/BIRmut

    Depositor

    Eric Fischer

    Article

    Hunkeler et al Science. 2023 Mar 17;379(6637):1105-1111

    Insert

    BIRC6 (BIRC6 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    C328S, C331S, L1332V, delta(1616-1666)

    Available Since

    March 29, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. DsRed -7e

    Plasmid

    #191765

    Purpose

    Expression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7e

    Depositor

    Daniel Gerlich

    Article

    Schneider et al Nature. 2022 Sep;609(7925):183-190. doi:

    Insert

    DsRed
    

    Tags

    N terminal fusion of negatively charged polypetod…

    Expression

    Bacterial and Mammalian

    Promoter

    CMV

    Available Since

    Feb. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. H1.M10.luc

    Plasmid

    #193706

    Purpose

    Expressed a H1-core mutant by introducing multiple 7SK elements (PSE, spacer, and TATA box)

    Depositor

    Elena Herrera-Carrillo

    Article

    Gao et al J Biol Chem. 2020 Nov 5. pii: S0021-9258

    Insert

    H1-core promoter with multiple 7SK domains (PSE, spacer, and TATA box) + N41
    

    Use

    Luciferase

    Expression

    Mammalian

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV.CBA.YFP.miR-E_shSrpk3-1

    Plasmid

    #180400

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-1 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-1 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. AAV.CBA.YFP.miR-E_shSrpk3-2

    Plasmid

    #180401

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-2 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. AAV.CBA.YFP.miR-E_shSrpk3-3

    Plasmid

    #180402

    Purpose

    Producing AAV that encodes mouse Srpk3 shRNA-3 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shSrpk3-3 (Srpk3 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AAV.CBA.YFP.miR-E_shStk16-2

    Plasmid

    #180392

    Purpose

    Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shStk16-2 (Stk16 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. AAV.CBA.YFP.miR-E_shStk16-3

    Plasmid

    #180393

    Purpose

    Producing AAV that encodes mouse Stk16 shRNA-2 with miR-E backbone

    Depositor

    Huda Zoghbi

    Article

    Lee et al J Clin Invest. 2022 May 2;132(9). pii: 1

    Insert

    shStk16-3 (Stk16 Mouse)
    

    Use

    AAV and RNAi

    Promoter

    CBA

    Available Since

    Nov. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pLL5.0-EGFP-HRasC20

    Plasmid

    #187252

    Purpose

    Lenticiral expression of EGFP-HRasC20

    Depositor

    Alpha Yap

    Article

    Katsuno-Kambe et al Elife. 2021 Oct 18;10. pii: 67915. doi:

    Insert

    HRasC20 (HRAS Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Promoter

    5' LTR

    Available Since

    Sept. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pST_YlHD (pBS0275)

    Plasmid

    #185153

    Purpose

    For the mammalian expression of the Yarrowia fungus protein YlHD. This is one of the bottom performing targets in our apoptosis assay (it induced apoptosis).

    Depositor

    Pamela Silver

    Article

    Veling et al ACS Synth Biol. 2022 Mar 18;11(3):1292-1

    Insert

    YlHD
    

    Expression

    Mammalian

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGEX-2T-CBP(1-287)

    Plasmid

    #182839

    Purpose

    CBP(1-287) was inserted into pGEX-2T with NdeI and NsiI sites. pGEX-2T-CBP(1-287) was used in the GST pull-down assay described in our paper (Tie et al. 2012 MCB, Tie et al 2014 Development)

    Depositor

    Peter Harte

    Article

    Tie et al Development. 2014 Mar;141(5):1129-39. do

    Insert

    CBP1-287
    

    Expression

    Bacterial

    Available Since

    April 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pK-eCFP

    Plasmid

    #176559

    Purpose

    Expression of eCFP for auxotrophic selection in the absence of histidine

    Depositor

    Markus Ralser

    Article

    Olin-Sandoval et al Nature. 2019 Aug;572(7768):249-253. doi:

    Insert

    eCFP
    

    Expression

    Yeast

    Promoter

    TDH1(GAP)

    Available Since

    Dec. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. CDS12_Cas9-NLS

    Plasmid

    #136122

    Purpose

    Puchta Arabidopsis codon-optimised Cas9 for CRISPR. CDS12 for N-term fusion with a CTAG

    Depositor

    Jim Haseloff

    Article

    Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

    Insert

    CDS12_AtcoCas9-NLS(noStop)
    

    Use

    Synthetic Biology

    Expression

    Plant

    Mutation

    BsaI/ SapI domesticated

    Available Since

    Nov. 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pGEMHE-bNup98-DN-NLS-mClover3

    Plasmid

    #171495

    Purpose

    T7 promotor drives in vitro transcription of mClover3-tagged bovine Nup98 DN mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Nup98-DN (NUP98 B. tarus (bovine))
    

    Use

    Pgehme

    Tags

    mClover3

    Promoter

    T7

    Available Since

    July 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. CMV BARK R106A p2A mCherry

    Plasmid

    #117688

    Purpose

    CMV-iBARK(R106A)-p2A-mCherry: Negative control mammalian expression vector for Gaq silencing

    Depositor

    Baljit Khakh

    Article

    Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

    Insert

    BARKrgs R106A peptide
    

    Tags

    HA / FLAG

    Expression

    Mammalian

    Mutation

    R106A

    Available Since

    May 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. CPH3566 (pDEST15_Spt6 1223-1451)

    Plasmid

    #91814

    Purpose

    Bacterial expression of residues 1223-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    deleted residues 1-1222

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. CPH1141 (pET151_Spt6 1247-1451)

    Plasmid

    #91815

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    deleted residues 1-1246

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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