We narrowed to 6,146 results for: cat.2
-
Plasmid#66509PurposeExpression of G protein-coupled receptors for PRESTO-Tango: parallel receptorome expression and screening via transcriptional output, with transcriptional activation following arrestin translocationDepositorInsertTAAR2 (TAAR2 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC91-pmax-dCas9VP64
Plasmid#48223PurposedCas9VP64 on pmax expression vectorDepositorInsertdCas9(D10A;H840A) fusion with VP64 activation domain
UseCRISPRTagsHA Tag and VP64ExpressionMammalianMutationD10A;H840A (catalytically inactive)PromoterCAGGSAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCS6-StaPLd-Casp9
Plasmid#111511PurposeExpresses a tandem caspase-9 dimer, governed by a StaPL(AI) module, in mammalian cells. [AI = asunaprevir inhibited].DepositorInsertStaPLd-Casp9
UseTagsExpressionMammalianMutationThe HCV NS3 protease carries V36M, T54A, and S122…PromoterCMVAvailable sinceJuly 3, 2018AvailabilityAcademic Institutions and Nonprofits only -
FUW-TetO-Dppa2
Plasmid#40799DepositorInsertDppa2 (Dppa2 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterMinimal CMV + TetOAvailable sinceOct. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
GFP-MYO10-BioID
Plasmid#194858PurposeExpress MYO10 tagged with eGFP (Nter) and BioID (Cter) in mammalian cells.DepositorInsertMYO10 (MYO10 Human)
UseTagsBioID and EGFPExpressionMammalianMutationMyoX contains a Q680R variant compared to the NCB…PromoterCMVAvailable sinceMarch 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLKO-shcIAP1-B
Plasmid#44131DepositorInsertc-IAP1 (BIRC2 Human)
UseLentiviral and RNAiTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-1_NbATML1-1pro
Plasmid#231149PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbATML1-1proDepositorInsertTREX2 and mobile gRNA targeting NbATML1-1pro
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
VirTREX2-HLDel-1_NbSTM-5'UTR
Plasmid#231148PurposeT-DNA encoding TRV2 with TREX2 and mobile gRNA targeting NbSTM-5?UTRDepositorInsertTREX2 and mobile gRNA targeting NbSTM-5?UTR
UseTagsExpressionPlantMutationPromoterPEBV sub-genomicAvailable sinceMarch 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
FgH1tUTG-sgRNA.FOXA-ZEB2_CRISPRd
Plasmid#216170PurposeExpress the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- FOXA.bs- ZEB2.locus
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
LRG-sgRNA.FOXA-ZEB2_CRISPRd_v2
Plasmid#216173PurposeExpress the gRNA targeting the FOXA-binding site in the human ZEB2 locus for the CRISPRd experimentDepositorInsertsgRNA- FOXA.bs- ZEB2.locus
UseLentiviralTagsEGFPExpressionMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCas9(D10A)
Plasmid#74495PurposeNicking Cas9 derived from pWT Cas9 (Addgene #44250). Ampicillin resistance marker, the tetracycline repressor (TetR), a ColE1 origin of replication and Streptococcus pyogenes Cas9 D10ADepositorInsertCas9 D10A
UseSynthetic BiologyTagsExpressionBacterialMutationD10APromoterpTetoAvailable sinceMay 16, 2016AvailabilityAcademic Institutions and Nonprofits only -
pENTR-ERKKTRClover
Plasmid#59138PurposeORF encoding ERK KTR mClover flanked by Gateway sequencesDepositorInsertERK Kinase Translocation Reporter (MAPK1 Human, Mouse)
UseTagsExpressionMutationPromoterAvailable sinceSept. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.3-3XFLAG-AGO2
Plasmid#136687PurposeExpresses human AGO2 with an N-terminal 3XFLAG tag in mammalian cells (using a CMV promoter)DepositorInsertargonaute RISC Catalytic Component 2 (AGO2 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceMarch 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-MS2
Plasmid#184839PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the MS2 phage genome and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the MS2 phage genome, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G
Plasmid#146392PurposeInsect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.DepositorInsertDmeIF4G-trunc (eIF4G1 Fly)
UseTagsExpressionInsectMutation322-1666 truncated version of elF4G sequence with…PromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pLenti-X1-Neo-GFP-ATL2-deltaTM
Plasmid#109021Purposestable lentiviral expression of cDNADepositorInsertATL2-deltaTM (ATL2 Human)
UseLentiviralTagseGFPExpressionMammalianMutationtruncated form without C-terminal ER transmembran…PromoterEIF-1a promoterAvailable sinceAug. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pPlacPbuCas13b-gRNA-T-deGFP
Plasmid#184840PurposeExpression of a single-spacer CRISPR array with spacer #1 targeting the mRNA of deGFP and expression of PbuCas13b in bacteria.DepositorInsertsingle-spacer CRISPR array with spacer #1 targeting the the mRNA of deGFP, PbuCas13b nuclease
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterJ23119 and lac promoterAvailable sinceOct. 5, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPICZ A-SLC-His+MFN2[∆TM]-Strep (SB260)
Plasmid#227609PurposeInducible coexpression of His-tagged SLC25A46 and Strep-tagged MFN2 lacking its transmembrane region in Pichia pastorisDepositorUseTags10xHis and Twin-StrepTagExpressionYeastMutationcontains aa 2-544, 651-757 only [TM deleted]PromoterAOX1Available sinceDec. 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
(189) pcDNA3.1-HA-nGFP-(EAAAK)4-OGT(4)
Plasmid#160739PurposeExpresses a truncated OGT (4.5 TPRs) fused to a GFP nanobodyDepositorInsertHA-nGFP-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
(137) pcDNA3.1-HA-nEPEA-(EAAAK)4-OGT(4)
Plasmid#160740PurposeExpresses a truncated OGT (4.5 TPRs) fused to the EPEA nanobodyDepositorInsertHA-nEPEA-(EAAAK)4-OGT(4)
UseTagsHA-TagExpressionMammalianMutationPromoterT7/CMVAvailable sinceMay 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_UBASH3A-1/1
Plasmid#91478PurposeProtein expression and purification of human SH3 domain construct UBASH3A-1/1DepositorInsertUBASH3A-1/1 (UBASH3A Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_SLA2-1/1
Plasmid#91450PurposeProtein expression and purification of human SH3 domain construct SLA2-1/1DepositorInsertSLA2-1/1 (SLA2 Human)
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 19, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PREX2_DEP
Plasmid#109893PurposeProtein expression and purification of PREX2_DEPDepositorInsertPREX2_DEP (PREX2 Human)
UseTagsExpressionBacterialMutationamino acids 369-474PromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
pHH0103_PREX2_DEP
Plasmid#109894PurposeProtein expression and purification of PREX2_DEPDepositorInsertPREX2_DEP (PREX2 Human)
UseTagsExpressionBacterialMutationamino acids 472-576PromoterAvailable sinceOct. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
TIAM2_PDZ_1
Plasmid#103952PurposeProtein expression and purification of TIAM2 PDZ domainDepositorInsertTIAM2 (TIAM2 Human)
UseTagsGSTExpressionBacterialMutationPromotertacAvailable sinceAug. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
E42_gRunx1_gRNA3_dTet_NGFR
Plasmid#189803PurposeRetroviral delivery of guide RNA against mouse Runx1DepositorInsertgRunx1_gRNA3 (Runx1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pC-HYG-YR
Plasmid#61765PurposeYeast recombinational cloning compatible Agrobacterium tumefaciens ternary vector containing hygromycin selectable marker on transfer DNA (TDNA).DepositorInsertsHygromycin
2 micron origin or replication and URA3 gene for S. cerevisiae
UseTernary vector for agrobacterium mediated transfo…TagsExpressionMutationPromotertrpC promoterAvailable sinceMay 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAC2-dual-dCas9VP48-sgExpression
Plasmid#48236PurposeDual expression construct expressing both dCas9VP48 and sgRNA from separate promotersDepositorInsertdCas9VP48
UseCRISPRTagsHA-tag and VP48ExpressionMammalianMutationD10A H840A (catalytically inactive)PromoterAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits only -
E42_gRunx1_gRNA1_dTet_NGFR
Plasmid#189802PurposeRetroviral delivery of guide RNA against mouse Runx1DepositorInsertgRunx1_gRNA1 (Runx1 Mouse)
UseRetroviralTagsExpressionMutationPromoterAvailable sinceOct. 27, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS NL4-3 envFS eGFP 5'CMV Δtat ΔTARx2_d2TetOp
Plasmid#101349Purpose2xTet Operator inserted between NFkB and Sp1 sites in U3 of HIV-1 delta tat with 5' CMV-R-U5. 5' and 3' TAR elements were mutated to: 5'-GGTCTCTCTGGTTAGACCAGAAAGGAGCATTGGAGCTCTCTGGCTAACTAGGGAACCC-3DepositorInsertNL4-3
UseLentiviralTagsExpressionMutationdelta tat delta TAR delta env GFP in Nef Tet ope…PromoterAvailable sinceMarch 14, 2019AvailabilityAcademic Institutions and Nonprofits only