We narrowed to 4,337 results for: U6 gRNA

1. pJW1883

   Plasmid

   #154331

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   ttTi5605 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pJW1850

   Plasmid

   #154328

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   ttTi5605 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pJW1849

   Plasmid

   #154327

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   ttTi4348 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Promoter

   Available since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJW1882

   Plasmid

   #154330

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   ttTi4348 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Promoter

   Available since

   Dec. 2, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pJW1851

   Plasmid

   #154329

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   cxTi10882 site targeting sgRNA (F+E) with K09B11.2 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pJW1884

   Plasmid

   #154332

   Purpose

   sgRNA Target Vector for CRISPR

   Depositor

   Jordan Ward

   Article

   Ashley et al Genetics. 2021 Mar 31;217(3). pii: 61045

   Insert

   cxTi10882 site targeting sgRNA (F+E) with R07E5.16 U6 promoter and 3'UTR
   

   Use

   CRISPR

   Tags

   Expression

   Worm

   Mutation

   F+E mutations in sgRNA

   Promoter

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. PL6-CS-WR

   Plasmid

   #122512

   Purpose

   construction for sgRNA transcription with Plasmodium falciparum U6 promotor

   Depositor

   Lubin Jiang

   Article

   Xiao et al Proc Natl Acad Sci U S A. 2019 Jan 2;116

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   PfU6

   Available since

   June 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSpdCas9-huTET1CD-T2A-GFP (PX458)

   Plasmid

   #129025

   Purpose

   targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-huTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A)

   Promoter

   CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpdCas9-hudTET1CD-T2A-GFP (PX458)

   Plasmid

   #129026

   Purpose

   inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-EGFP and cloning backbone for sgRNA

   Depositor

   Julia K Polansky

   Article

   Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

   Insert

   dCas9-hudTET1CD, SgRNA cloning site
   

   Use

   Tags

   HA-Tag, NLS and T2A-EGFP

   Expression

   Mammalian

   Mutation

   dCas9 (D10A;H840A), catalytic domain huTET1 inact…

   Promoter

   CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

   Available since

   Jan. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pSpdCas9-huTET1CD-T2A-mCherry(PX458)

    Plasmid

    #129027

    Purpose

    targeted DNA demethylation, expression of dCas9-huTET1CD-T2A-mCherry and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-huTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A)

    Promoter

    CbH (for dCas9-huTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pSpdCas9-hudTET1CD-T2A-mCherry(PX458)

    Plasmid

    #129028

    Purpose

    inactivated control (targeted DNA demethylation),expression of dCas9-hudTET1CD-T2A-mCherry and cloning backbone for sgRNA

    Depositor

    Julia K Polansky

    Article

    Kressler et al Front Immunol. 2021 Jan 7;11:609891. doi

    Insert

    dCas9-hudTET1CD, SgRNA cloning site
    

    Use

    Tags

    HA-Tag, NLS and T2A-mCherry

    Expression

    Mammalian

    Mutation

    dCas9 (D10A;H840A), catalytic domain huTET1 inact…

    Promoter

    CbH (for dCas9-hudTET1CD-T2A-EGFP) U6 (for sgRNA)

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. PX333-T2A-EGFP

    Plasmid

    #197417

    Purpose

    SpCas9-T2A-EGFP with dual sgRNA cloning backbones

    Depositor

    Yeh-Hsing Lao

    Article

    Yoshinaga et al Nano Lett. 2023 Jan 17. doi: 10.1021/acs

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CBh; U6

    Available since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pWN_U6-B2M-miniGag

    Plasmid

    #228957

    Purpose

    miniEDV production plasmid. Expresses the miniGag (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. c3GIC9

    Plasmid

    #62191

    Purpose

    col1a1 targeting vector for inducible [TRE3G]-GFP-IRES-Cas9 expression. Contains NsiI cloning site for U6-sgRNA cassettes

    Depositor

    Lukas Dow

    Article

    Dow et al Nat Biotechnol. 2015 Apr;33(4):390-4. do

    Insert

    humanized S. Pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3x FLAG

    Expression

    Mutation

    Promoter

    TRE3G

    Available since

    Aug. 10, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLentiGuide_GFP_sgEPAS1#2

    Plasmid

    #183120

    Purpose

    pLentiGuide modified to express GFP and an sgRNA targeting EPAS1

    Depositor

    William Kaelin

    Article

    Stransky et al Proc Natl Acad Sci U S A. 2022 Apr 5;119

    Insert

    EPAS1-2
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Aug. 24, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. UGPC

    Plasmid

    #138954

    Purpose

    Lentiviral vector for co-expressing Cre recombinase and sgRNA

    Depositor

    Nadya Dimitrova

    Article

    Olivero et al Mol Cell. 2020 Feb 20;77(4):761-774.e8.

    Type

    Empty backbone

    Use

    Cre/Lox and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6 and PGK

    Available since

    Aug. 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pWN_U6-TRAC-miniGag

    Plasmid

    #228960

    Purpose

    miniEDV production plasmid. Expresses the miniGag polypeptide (CAG promoter) and sgRNA (human U6 promoter)

    Depositor

    Jennifer Doudna

    Article

    Ngo et al Proc Natl Acad Sci U S A. 2025 Jan 7;122

    Insert

    miniGag
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV/Guide-mCherry

    Plasmid

    #170543

    Purpose

    Expresses mCherry and contains a cassette for U6 driven sgRNA transcription

    Depositor

    Jacob Giehm Mikkelsen

    Article

    Thomsen et al Mol Ther Nucleic Acids. 2022 Aug 4;29:56

    Insert

    mCherry
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Ef1-alpha

    Available since

    Jan. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p2T-CAG-MCS-P2A-GFP-PuroR

    Plasmid

    #107186

    Purpose

    Base plasmid for cloning gene duplication alleles with eGFP reporter.

    Depositor

    Richard Sherwood

    Article

    Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Dec. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLentiCRISPRRv2_sgTom

    Plasmid

    #218346

    Purpose

    CRISPR KO plasmid for tdTomato in human cell lines

    Depositor

    Seth Parker

    Article

    Johal et al Cell Rep. 2024 Dec 24;43(12):115069. doi

    Insert

    Inserted sgRNA targetting tdTomato
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge