We narrowed to 12,703 results for: NUC

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3/Flag-WTAP

Plasmid

#53741

Purpose

For expression of WTAP (Wilms tumor 1 associated protein) in human cells

Depositor

Chuan He

Article

Liu et al Nat Chem Biol. 2014 Feb;10(2):93-5. doi:

Insert

Wilms tumor 1 associated protein (WTAP Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

T7

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pMALcPP-MS2cp

Plasmid

#203351

Purpose

Expresses His/MBP-tagged MS2-CP V75E/A81G variant in bacteria

Depositor

Thomas Cech

Article

Hemphill et al Proc Natl Acad Sci U S A. 2023 Jun 27;12

Insert

MS2 coat protein

Tags

Hx6-MBP

Expression

Bacterial

Mutation

V75E and A81G, to prevent capsid assembly

Available Since

July 24, 2023

Availability

Academic Institutions and Nonprofits only

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pX459V2.0-SpCas9-HF1

Plasmid

#108293

Purpose

pX459 V2.0 (Plasmid #62988) with the N497A, R661A, Q695A, and Q926A mutations

Depositor

Yuichiro Miyaoka

Article

Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pcdna nesprin TS

Plasmid

#68127

Purpose

nesprin tension sensor

Depositor

Daniel Conway

Article

Arsenovic et al Biophys J. 2016 Jan 5;110(1):34-43. doi:

Insert

mouse nesprin mini 2G (Syne2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp15-mNeongreen

Plasmid

#182389

Purpose

Bacterial expression of Vesicle Nucleating peptide15-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp15-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pCRISPR-Cas3

Plasmid

#209427

Purpose

Plasmid for CASCADE-Cas3 based genome engineering of streptomycetes

Depositor

Tilmann Weber

Article

Whitford et al Nucleic Acids Res. 2025 Mar 20;53(6):gka

Insert

codon optimized minimal type I-C CASCADE-Cas3 from Pseudomonas aeruginosa

Use

CRISPR and Synthetic Biology

Promoter

PtipA; crRNA under control of PermE*

Available Since

March 13, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C1-ER alpha

Plasmid

#28230

Purpose

Mammalian expression of Estrogen Receptor alpha fused to EGFP

Depositor

Michael Mancini

Article

Stenoien et al Mol Endocrinol. 2000 Apr . 14(4):518-34.

Insert

Estrogen Receptor alpha (ESR1 Human)

Tags

EGFP

Expression

Mammalian

Available Since

April 7, 2011

Availability

Academic Institutions and Nonprofits only

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pTRIPZ shNS

Plasmid

#127696

Purpose

Doxycyclin inducible pTRIPZ lentivirus vector containing a non-silencing sequence for both human and mouse

Depositor

Sandra Demaria

Article

Vanpouille-Box et al Nat Commun. 2017 Jun 9;8:15618. doi: 10.

Insert

shNS

Use

Lentiviral

Expression

Mammalian

Available Since

Nov. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSP2-48-merTetO-EFS-BLaR

Plasmid

#118712

Purpose

Contains an optimized 48-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-SpRY-ABE8e

Plasmid

#185671

Purpose

Expresses SpRY-ABE8e in mammalian cells

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Insert

ecTadA(8e)-nSpRY

Use

CRISPR

Expression

Mammalian

Mutation

SpRY D10A

Available Since

Sept. 26, 2022

Availability

Academic Institutions and Nonprofits only

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eSpCas9(1.1)

Plasmid

#71814

Purpose

Expresses high specificity SpCas9. Px330-like plasmid.

Depositor

Feng Zhang

Article

Slaymaker et al Science. 2015 Dec 1. pii: aad5227.

Has Service

Cloning Grade DNA

Insert

enhanced specificity Cas9 (1.1)

Use

CRISPR

Tags

3xFLAG

Expression

Mammalian

Mutation

K848A, K1003A, & R1060A

Promoter

CBh

Available Since

Dec. 10, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-APEX.NES-P2A-EGFP

Plasmid

#182824

Purpose

Cre-dependent expression of cytosolic APEX

Depositor

Yevgenia Kozorovitskiy

Article

Dumrongprechachan et al Nat Commun. 2021 Aug 11;12(1):4855. doi:

Insert

APEX.NES-P2A-EGFP

Use

AAV

Tags

EGFP reporter (P2A)

Promoter

EF1a

Available Since

May 16, 2022

Availability

Academic Institutions and Nonprofits only

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pCXLE-mCherry

Plasmid

#193293

Purpose

episomal vector expressing mCherry fluorescent protein

Depositor

Hans Schöler

Article

MacCarthy et al Cell Stem Cell. 2024 Jan 4;31(1):127-147

Insert

mCherry

Expression

Mammalian

Promoter

CAG

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pRha-ABE7.10-SpCas9-NG

Plasmid

#170663

Purpose

Expresses the base editor ABE7.10-SpCas9-NG in bacterial cells

Depositor

David Liu

Article

Krishnamurthy et al Nucleic Acids Res. 2021 Oct 11;49(18):10

Insert

ABE7.10-SpCas9-NG

Tags

8xHIS and BP-NLS

Expression

Bacterial

Promoter

pRhaBAD

Available Since

Oct. 12, 2022

Availability

Academic Institutions and Nonprofits only

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R26-H2B-EGFP HR donor vector

Plasmid

#137925

Purpose

R0SA26 knock-in vector with homologous recombination-based method

Depositor

Hiroshi Kiyonari

Article

Abe et al Cell Rep. 2020 May 19;31(7):107653. doi:

Insert

SA-H2B-EGFP-bpA with homology arms

Use

Mouse Targeting

Available Since

May 19, 2020

Availability

Academic Institutions and Nonprofits only

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pSP2-96-merTetO-EFS-BLaR

Plasmid

#118713

Purpose

Contains an optimized 96-mer TetO repeat for imaging of desired loci

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Inserts

Blasticidin resistance gene
EFS-NS promoter

Expression

Mammalian

Promoter

EFS-NS

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3 p53 WT

Plasmid

#69003

Purpose

mammalian expression of wild type human p53

Depositor

David Meek

Article

Loughery et al Nucleic Acids Res. 2014 Jul;42(12):7666-

Insert

p53 (TP53 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only

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pcDL-hu FceRI alpha

Plasmid

#8365

Depositor

Jean Kinet

Article

Kochan et al Nucleic Acids Res 1988 Apr 25;16(8):3584

Insert

human Fc epsilon RI alpha cDNA (FCER1A Human)

Expression

Mammalian

Available Since

May 24, 2006

Availability

Academic Institutions and Nonprofits only

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pET-28b-Cas9-His

Plasmid

#47327

Purpose

For in vitro expression and purification of Cas9 protein

Depositor

Alex Schier

Article

Gagnon et al PLoS One. 2014 May 29;9(5):e98186. doi:

Insert

Cas9

Use

CRISPR

Tags

His

Expression

Bacterial

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA6A-EGFR ECD (1-644)

Plasmid

#42666

Depositor

Mien-Chie Hung

Article

Hsu et al J Biol Chem. 2007 Apr 6;282(14):10432-40

Insert

EGFR ECD (1-644) (EGFR Human)

Tags

6xHis and Myc

Expression

Mammalian

Available Since

June 17, 2013

Availability

Academic Institutions and Nonprofits only

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Flag-GFP11-pDEST-C

Plasmid

#118367

Purpose

Bimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.

Depositor

Maria Vartiainen

Article

Viita et al J Cell Sci. 2019 Apr 17;132(8). pii: jcs

Type

Empty backbone

Use

Destination

Tags

GFP11-FLAG

Expression

Mammalian

Available Since

May 1, 2019

Availability

Academic Institutions and Nonprofits only

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pCMVTNT-T7-KPNA4

Plasmid

#26680

Depositor

Bryce Paschal

Article

Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

Insert

Karyopherin Alpha 4 (KPNA4 Human)

Tags

T7

Expression

Mammalian

Available Since

Nov. 10, 2010

Availability

Academic Institutions and Nonprofits only

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pDIV270

Plasmid

#226181

Purpose

Expresses Cas9 and gRNA targeting KU70 gene in K. phaffii.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pRH2502

Plasmid

#84379

Purpose

Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

Depositor

Robert Husson

Article

Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

Insert

dcas9

Use

CRISPR

Expression

Bacterial

Mutation

Aspartate at position 10 changed to Alanine, Hist…

Promoter

uv15tetO

Available Since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp6-mNeongreen

Plasmid

#182388

Purpose

Bacterial expression of Vesicle Nucleating peptide6-mNeongreen fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp6-mNeongreen-His

Tags

Vesicle Nucleating peptide (VNp)

Expression

Bacterial

Promoter

T7

Available Since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pDIV272

Plasmid

#226183

Purpose

Expresses Cas9 and gRNA targeting uidA gene of the pDIV643 derived cassette.

Depositor

Uffe Mortensen

Article

Strucko et al FEMS Yeast Res. 2024 Aug 23:foae026. doi

Insert

tRNA-sgRNA-tRNA

Use

CRISPR

Expression

Bacterial and Yeast

Promoter

TEF1p (Komagataella phaffii)

Available Since

Oct. 15, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-hXRCC1

Plasmid

#70759

Purpose

bacterial expression of human XRCC1

Depositor

Primo Schaer

Article

Schuermann et al Nucleic Acids Res. 2016 Jan 4. pii: gkv1

Insert

XRCC1 (XRCC1 Human)

Tags

6-HIS

Expression

Bacterial

Promoter

T7/lac operon

Available Since

Jan. 14, 2016

Availability

Academic Institutions and Nonprofits only

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EGFP-DCX

Plasmid

#32852

Depositor

Joseph Gleeson

Article

Tanaka et al J Cell Biol. 2004 Jun 7. 165(5):709-21.

Insert

DCX (DCX Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 27, 2011

Availability

Academic Institutions and Nonprofits only

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pJEC819

Plasmid

#190815

Purpose

CRISPRa backbone plasmid harboring BbsI sites for easy cloning of sgRNA targeting regions via Golden gate.

Depositor

James Chappell

Article

Ameruoso et al Nucleic Acids Res. 2022 Jul 22;50(13):77

Insert

dCas9-XTEN-rpoA

Use

CRISPR and Synthetic Biology

Tags

XTEN (linker), followed by the N-terminal domain …

Promoter

SP30

Available Since

Sept. 30, 2022

Availability

Academic Institutions and Nonprofits only

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