We narrowed to 11,766 results for: nsf

1. AAV-hSyn-Axon-jGCaMP8s

   Plasmid

   #172720

   Purpose

   AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8s.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   Axon-jGCaMP8s
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Dec. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. IL1RN gRNA1

   Plasmid

   #113130

   Purpose

   Expresses gRNA targeting the IL1RN promoter (SpyCas9 scaffold)

   Depositor

   Dominik Niopek

   Article

   Bubeck et al Nat Methods. 2018 Oct 30. pii: 10.1038/s

   Insert

   IL1RN guideRNA 1 (SpCas9 scaffold), co-expressed GFP (transfection marker)
   

   Use

   AAV, CRISPR, and Synthetic Biology

   Expression

   Mammalian

   Promoter

   U6 for gRNA expression, RSV for GFP expression

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSCALPS-FLAG-HA-mmuMettl3 APPA

   Plasmid

   #207124

   Purpose

   Lentiviral plasmid to express mouse Mettl3 APPA mutant

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   Mettl3 (Mettl3 Mouse)
   

   Use

   Lentiviral

   Tags

   FLAG-HA

   Mutation

   D395A and W398A

   Promoter

   SSFV

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV-SYP1-miniSOG-citrine

   Plasmid

   #50971

   Purpose

   AAV2 transfer vector containing the InSynC (SYP1-miniSOG-citrine) construct

   Depositor

   Roger Tsien

   Article

   Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

   Insert

   Synaptophysin-miniSOG-citrine (Syp Rat)
   

   Use

   AAV

   Tags

   citrine and miniSOG

   Promoter

   human synapsin promoter

   Available Since

   Feb. 5, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV-hSyn-Axon-jGCaMP8m

   Plasmid

   #172719

   Purpose

   AAV transfer plasmid for neuronal expression of axon-targeted jGCaMP8m.

   Depositor

   Marianne Fyhn

   Article

   Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

   Insert

   Axon-jGCaMP8m
   

   Use

   AAV

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   hSyn

   Available Since

   Dec. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFR G719S

   Plasmid

   #11013

   Purpose

   Retroviral construct for expressing human EGFR with G719S mutation in human cells

   Depositor

   Matthew Meyerson

   Article

   Greulich et al PLoS Med. 2005 Oct 4. 2(11):e313.

   Insert

   EGFR G719S (EGFR Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   G719S

   Available Since

   Dec. 2, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pCI-neo-Flag-DHHS

   Plasmid

   #85813

   Purpose

   To rescue the expression of DHHC5 catalytically inactive mutant in the knockdown cell lines

   Depositor

   Sandra Hofmann

   Article

   Tian et al Mol Cancer Res. 2015 Apr;13(4):784-94. d

   Insert

   ZDHHC5 C134S mutant (Zdhhc5 Mouse)
   

   Tags

   Flag

   Expression

   Mammalian

   Available Since

   Jan. 13, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pSB90 - pAGM4723_VirGN54D

   Plasmid

   #123187

   Purpose

   Golden Gate (MoClo; Weber et al., 2011) Level 2 acceptor vector with increased virulence in Agrobacteria

   Depositor

   Erin Cram, Carolyn Lee-Parsons

   Article

   Mortensen et al Front Plant Sci. 2019 Jun 11;10:755. doi

   Type

   Empty backbone

   Use

   Level 2 acceptor vector for expression in plants

   Expression

   Plant

   Mutation

   VirGN54D for increase virulence

   Available Since

   May 29, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBABE-hLin28B

   Plasmid

   #26358

   Depositor

   George Daley

   Article

   Viswanathan et al Nat Genet. 2009 Jul . 41(7):843-8.

   Insert

   lin-28 homolog B (LIN28B Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Nov. 3, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUCmini-iCAP-PHP.B3

    Plasmid

    #103004

    Purpose

    non-standard AAV2 rep-AAV-PHP.B3 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B3 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pUCmini-iCAP-PHP.B2

    Plasmid

    #103003

    Purpose

    non-standard AAV2 rep-AAV-PHP.B2 cap plasmid with AAV cap expression controlled by a tTA-TRE amplification system

    Depositor

    Viviana Gradinaru

    Article

    Deverman et al Nat Biotechnol. 2016 Feb;34(2):204-9. do

    Insert

    Synthetic construct isolate AAV-PHP.B2 VP1 gene
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    p41

    Available Since

    Dec. 15, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDEST47-ARL2-GFP

    Plasmid

    #67396

    Purpose

    Mammalian expression of hArl2 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL2 (ARL2 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. AAV-SYP1-miniSOG-T2A-mCherry

    Plasmid

    #50972

    Purpose

    AAV2 transfer vector containing the InSynC (SYP1-miniSOG-T2A-mCherry) construct

    Depositor

    Roger Tsien

    Article

    Lin et al Neuron. 2013 Jul 24;79(2):241-53. doi: 1

    Has Service

    AAV8

    Insert

    Synaptophysin-miniSOG-T2A-mCherry (Syp Rat)
    

    Use

    AAV

    Tags

    T2A-mCherry and miniSOG

    Promoter

    human synapsin promoter

    Available Since

    Feb. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pmirGLO-3'UTR mouse Il6

    Plasmid

    #207126

    Purpose

    Luciferase vector containing 3'UTR for mouse Il6

    Depositor

    Silvia Monticelli

    Article

    Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

    Insert

    Interleukin 6 (Il6) (Il6 Mouse)
    

    Use

    Luciferase

    Expression

    Mammalian

    Promoter

    PGK promoter

    Available Since

    Dec. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. gh141

    Plasmid

    #106811

    Purpose

    expression of gRNA targeting OGT

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    OGT (OGT Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pENTR4 CDS A. thaliana PHOT1

    Plasmid

    #209187

    Purpose

    pENTR4 plasmid with CDS of Arabidopsis thaliana PHOTOTROPIN 1 (PHOT1, AT3G45780.1) without stop codon for C-terminal epitope tags

    Depositor

    Lennart Wirthmueller

    Article

    Simranjit et al bioRxiv 2023.09.25.558804

    Insert

    AT3G45780.1 (PHOT1 Mustard Weed)
    

    Use

    Gateway-compatible entry vector

    Promoter

    none

    Available Since

    Nov. 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCAGGS-4493NES

    Plasmid

    #138373

    Purpose

    Booster-PKA, a Red-Shifted Genetically Encoded FRET Biosensor for monitoring PKA activity.

    Depositor

    Michiyuki Matsuda

    Article

    Watabe et al ACS Sens. 2020 Feb 26. doi: 10.1021/acss

    Insert

    Booster-PKA
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. EGFP-IpgD

    Plasmid

    #183666

    Purpose

    N-terminally tagged Shigella flexneri IpgD for mammalian expression

    Depositor

    Sergio Grinstein

    Article

    Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

    Insert

    IpgD
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. β₂AR-mCFP

    Plasmid

    #38260

    Depositor

    Robert Lefkowitz

    Article

    Violin et al J Biol Chem. 2006 Jul 21;281(29):20577-8

    Insert

    β₂AR (ADRB2 Human)
    

    Tags

    Flag, mCFP, and signal peptide sequence (MKTIIALS…

    Expression

    Mammalian

    Mutation

    diglycine linker (replacing terminator codon)

    Promoter

    CMV

    Available Since

    Aug. 23, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pGEX-2T-GST-eIF4E K119A

    Plasmid

    #112818

    Purpose

    Expresses N-terminally GST-tagged mouse Eif4e K119A in bacterial cells

    Depositor

    Daniel Schoenberg

    Article

    Trotman et al Nucleic Acids Res. 2017 Oct 13;45(18):10

    Insert

    Eif4e (Eif4e Mouse)
    

    Tags

    GST

    Expression

    Bacterial

    Mutation

    K119 mutated to A, increasing affinity for cap st…

    Promoter

    T7

    Available Since

    Jan. 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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