We narrowed to 9,400 results for: Coli

1. pET-6xHis-GFP(-6)-L3

   Plasmid

   #205029

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-L3
   

   Use

   Tags

   KGRRGKKSRKGRRGKKSRKGRRGKKSRK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pET-6xHis-GFP(-6)-H1

   Plasmid

   #205030

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H1
   

   Use

   Tags

   KKRRRKK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pET-6xHis-GFP(-6)-H2

   Plasmid

   #205031

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H2
   

   Use

   Tags

   KKRRRKKKRRRKK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pET-6xHis-GFP(-6)-H3

   Plasmid

   #205032

   Purpose

   Fluorescent protein with charge-patterned cationic peptide to promote biomolecular condensate formation in E. coli

   Depositor

   Allie Obermeyer

   Article

   Liao et al ACS Synth Biol. 2024 Feb 3. doi: 10.1021

   Insert

   GFP(-6)-H3
   

   Use

   Tags

   KKRRRKKKRRRKKKRRRKK and MGHHHHHGGA

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Feb. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pET28-RPL3-Δ1~34,Δ215~284-TwStrep

   Plasmid

   #208630

   Purpose

   for E. coli expression of truncated human RPL3 (ENST00000216146), aa35~398 with deletion of 215~284, fused with Twin Strep tag.

   Depositor

   Quanfu Ma

   Insert

   human RPL3 (ENST00000216146) Δ1~34, Δ215~284 (RPL3 Human)
   

   Use

   Tags

   6xHis, Twin Strep, and mEGFP(A206K)

   Expression

   Bacterial

   Mutation

   Δ1~34,Δ215~284

   Promoter

   T7

   Available Since

   Jan. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pTatABmNeonC

   Plasmid

   #178464

   Purpose

   pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatB.

   Depositor

   Siegfried Musser

   Article

   Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

   Insert

   TatA, TatBmNeon, TatC
   

   Use

   Tags

   mNeonGreen fused to TatB

   Expression

   Bacterial

   Mutation

   Promoter

   araC

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTatABCmNeon

   Plasmid

   #178465

   Purpose

   pTatABC with an mNeonGreen fluorescent protein tag at the C-terminal end of TatC.

   Depositor

   Siegfried Musser

   Article

   Sharma et al Commun Biol. 2022 Sep 19;5(1):988. doi:

   Insert

   TatA, TatB, TatCmNeon
   

   Use

   Tags

   mNeonGreen fused to TatC

   Expression

   Bacterial

   Mutation

   Promoter

   araC

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pE5772

   Plasmid

   #170779

   Purpose

   expression of hybrid proteins in E. coli

   Depositor

   Anthony Schryvers

   Article

   Chaudhuri et al Methods Mol Biol. 2022;2414:115-140. doi

   Insert

   GentR
   

   Use

   Tags

   polyhisbioMbpTEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   Oct. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSecEffector wo TEVp

   Plasmid

   #172892

   Purpose

   atc inducible (in any E. coli strain) BxbI and arabinose inducible TP901

   Depositor

   Urartu Seker

   Article

   Ahan et al ACS Synth Biol. 2019 Apr 19;8(4):686-696

   Inserts

   Bxb1
   TP901
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   nTETO and pBAD /AraC

   Available Since

   July 29, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDest-894 (attR4-attR2)

    Plasmid

    #161893

    Purpose

    Multisite Gateway (attR4-attR2) vector for expression of multiple proteins in E. coli

    Depositor

    Dominic Esposito

    Article

    Wall et al Methods Mol Biol. 2014;1116:193-208. doi

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 21, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pEPMY1CB0001

    Plasmid

    #162317

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-CCAT. Generates plasmids for T7-driven expression in E. coli. Contains mRFP1 reporter.

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pEPQD0KN0025

    Plasmid

    #162283

    Purpose

    Acceptor for golden gate assembly with BsaI overhangs GCTT-AATG. Generates plasmids for T7-driven E. coli cell-free protein synthesis

    Depositor

    Nicola Patron

    Article

    Dudley et al Synth Biol (Oxf). 2021 Sep 11;6(1):ysab0

    Type

    Empty backbone

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Jan. 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pSUI-SynP-CAS1sg

    Plasmid

    #154341

    Purpose

    Expression of the sgRNA targeting to the E. coli can gene

    Depositor

    I-Son Ng

    Article

    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

    Insert

    CAS1 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 19, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pSUI-SynP-CAS2sg

    Plasmid

    #154342

    Purpose

    Expression of the sgRNA targeting to the E. coli can gene

    Depositor

    I-Son Ng

    Article

    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

    Insert

    CAS2 sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pSUI-SynP-CANTsg

    Plasmid

    #154343

    Purpose

    Expression of the sgRNA targeting to the E. coli can gene

    Depositor

    I-Son Ng

    Article

    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

    Insert

    CANT sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pSUI-SynP-CAPsg

    Plasmid

    #154344

    Purpose

    Expression of the sgRNA targeting to the E. coli can gene

    Depositor

    I-Son Ng

    Article

    Tan et al Biotechnol Bioeng. 2020 May 27. doi: 10.

    Insert

    CAP sgRNA
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28a-SznF E215A

    Plasmid

    #139565

    Purpose

    expresses SznF E215A in E. coli

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF E215A
    

    Use

    Tags

    hexahistidine

    Expression

    Bacterial

    Mutation

    changed glutamate 215 to alanine

    Promoter

    T7

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28a-SznF

    Plasmid

    #139564

    Purpose

    expresses SznF in E. coli

    Depositor

    Emily Balskus

    Article

    Ng et al Nature. 2019 Feb;566(7742):94-99. doi: 1

    Insert

    SznF
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    March 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pAW092

    Plasmid

    #113250

    Purpose

    E. coli expression vector for His-MBP-S. pyogenes Cas1 H205A

    Depositor

    Jennifer Doudna

    Article

    Wright et al Nat Struct Mol Biol. 2016 Oct;23(10):876

    Insert

    S. pyogenes Cas1
    

    Use

    Tags

    6xHis-MBP

    Expression

    Bacterial

    Mutation

    H205A

    Promoter

    T7

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pBbA5k-AcrB/AcrD/AcrFa, ABO_0964

    Plasmid

    #86202

    Purpose

    Expresses efflux pump in E. coli to provide resistance to biofuel (medium copy)

    Depositor

    Mary Dunlop

    Article

    Turner et al ACS Synth Biol. 2015 Oct 16;4(10):1056-6

    Insert

    Efflux pump
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    ATCGTTTAGGCACCCCAGGCTTTACACTTTATGCTTCCGGCTCGTATAA…

    Available Since

    March 30, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pHL 309

    Plasmid

    #64906

    Purpose

    KanR + ColE1 + PLlacO-1::RBS(st7):rfp::RBS(st7):cfp::RBS(st7):yfp::T1 terminator

    Depositor

    Han Lim

    Article

    Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8

    Type

    Empty backbone

    Use

    Synthetic Biology; E. coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

22. pHL 1988

    Plasmid

    #64908

    Purpose

    KanR + ColE1 + PLlacO-1::RBS(st7):cfp::Asp terminator + PLlacO-1::RBS(st7):psu::T1 terminator

    Depositor

    Han Lim

    Article

    Hussein et al Biochim Biophys Acta. 2015 May 14;1849(8

    Type

    Empty backbone

    Use

    Synthetic Biology; E. coli

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    July 21, 2015

    Availability

    Academic Institutions and Nonprofits only

23. pCVD453

    Plasmid

    #66154

    Purpose

    cloning of E. coli E2348/69 left end of the LEE region, contains rorf1 and espG.

    Depositor

    James Kaper

    Article

    McDaniel et al Proc Natl Acad Sci U S A. 1995 Feb 28;92

    Insert

    LEE probe A rorf1, espG
    

    Use

    Cloning vector

    Tags

    Expression

    Mutation

    Promoter

    none

    Available Since

    June 18, 2015

    Availability

    Academic Institutions and Nonprofits only

24. pJT104

    Plasmid

    #25088

    Depositor

    Christopher Voigt

    Article

    Tabor et al Cell. 2009 Jun 26. 137(7):1272-81.

    Insert

    Edge detector delta luxI
    

    Use

    Synthetic Biology

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. enz.043-SETD7-Y305F

    Plasmid

    #154045

    Purpose

    Expresses SETD7 (Y305F) in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Histone-lysine N-methyltransferase SETD7 (SETD7 Human)
    

    Use

    Tags

    His, MBP

    Expression

    Bacterial

    Mutation

    changed tyrosine 305 to phenylalanine

    Promoter

    T7

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pAKW.119-R5-TEV-SUMO-His

    Plasmid

    #154050

    Purpose

    Expresses R5 peptide in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Silaffin R5 peptide
    

    Use

    Tags

    SUMO and His tag

    Expression

    Bacterial

    Mutation

    Promoter

    T5LacO

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pAKW.111A-SETD7

    Plasmid

    #154052

    Purpose

    Expresses SETD7 (Y305F) in E. coli

    Depositor

    Christopher Voigt

    Article

    Wallace et al Advanced Functional Materials 2020

    Insert

    Histone-lysine N-methyltransferase SETD7 (SETD7 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    changed tyrosine 305 to phenylalanine

    Promoter

    pTet*

    Available Since

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pGEX6P1-GFP-Nanobody

    Plasmid

    #61838

    Purpose

    Expresses GST-tagged anti-GFP Nanobody in E. coli cells.

    Depositor

    Kazuhisa Nakayama

    Article

    Katoh et al J Cell Sci. 2015 May 11. pii: jcs.168740

    Insert

    GFP-Nanobody
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pJK225

    Plasmid

    #71124

    Purpose

    Expression of Acetobacter aceti 1023 citrate synthase (AaCSH6) in E. coli

    Depositor

    T. J. Kappock

    Article

    Francois et al Biochemistry. 2006 Nov 14;45(45):13487-9

    Insert

    citrate synthase
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available Since

    Jan. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pRPF185

    Plasmid

    #106367

    Purpose

    C. difficile inducible expression system. Shuttle plasmid containing a tetracycline-inducible gusA

    Depositor

    Robert Fagan, Neil Fairweather

    Article

    Fagan et al J Biol Chem. 2011 Aug 5;286(31):27483-93

    Insert

    Term(fdx)-Ptet-gusA-Term(slpA) (gusA )
    

    Use

    E. coli - c. difficile shuttle vector

    Tags

    Expression

    Mutation

    codon optimised for C. difficile

    Promoter

    Ptet

    Available Since

    March 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge