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We narrowed to 78,817 results for: TRI

Showing: 5761 - 5790 of 78817 results

  1. pEGFP-C3-PLK4-K41M-S285A-T289A-3xFLAG

    Plasmid
    #69842
    Purpose
    This plasmid encodes kinase dead PLK4 isoform 1 carrying K41M and S285A/T289A mutations with an N-terminal EGFP tag and C-terminal 3xFLAG tag
    Depositor
    Michel Bornens
    Article
    Sillibourne et al Mol Biol Cell. 2010 Feb 15;21(4):547-61.
    Insert
    PLK4 (PLK4 Human)
    Use
    Tags
    3xFLAG tag and EGFP
    Expression
    Mammalian
    Mutation
    K41M-S285A-T289A
    Promoter
    CMV
    Available since
    Nov. 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    69842-plasmid-map-sequence-id-123881

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  2. pcDNA3.1_mGFP-FGFR3-K650E

    Plasmid
    #191757
    Purpose
    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-K650E mutation (associated with TD2; target mutation: c.1948A>G; silent mutation added on purpose: c.1938C>T)
    Depositor
    Irene Tiemann-Boege
    Article
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Insert
    mGFP-FGFR3IIIc
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    c.1948A>G (p.K650E) + silent mutation c.1938C&…
    Promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191757-plasmid-map-sequence-id-382279

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  4. pAcBac1-Ma-sfGFP WT

    Plasmid
    #197567
    Purpose
    Expression of sfGFP WT with Methanomethylophilus alvus (Ma) Pyl-tRNA for the encoding of non-canonical amino acids at TAG codons in HEK293 cells
    Depositor
    Ryan Mehl
    Article
    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3
    Inserts
    sfGFP WT
    M. alvus Pyl-tRNA (6) (4x copies)
    Use
    Tags
    V5-His6
    Expression
    Mammalian
    Mutation
    Promoter
    CMV and U6/H1
    Available since
    April 4, 2023
    Availability
    Academic Institutions and Nonprofits only
    197567-plasmid-map-sequence-id-389420

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  7. pTRF.1 udsVenus (P_JUND)

    Plasmid
    #58692
    Purpose
    A rapidly responsive reporter of endogenous JUND promoter activity (Lentiviral expression vector for reporter ultradestabilized Venus)
    Depositor
    Kevin Janes
    Article
    Wang et al Nat Cell Biol. 2014 Apr;16(4):345-56. do
    Insert
    endogenous promoter of JUND (JUND Human)
    Use
    Lentiviral
    Tags
    udsVenus
    Expression
    Mammalian
    Mutation
    Promoter
    Available since
    Aug. 11, 2014
    Availability
    Academic Institutions and Nonprofits only

  11. pMTB2-NLS-BirA-2A-mCherry_Ras

    Plasmid
    #80067
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA) with NLS, a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80067-plasmid-map-sequence-id-152621

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  12. pMTB2-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80066
    Purpose
    Beta-actin2 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Beta-actin2
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80066-plasmid-map-sequence-id-152619

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  14. 139H2_HC

    Plasmid
    #206201
    Purpose
    For recombinant expression of the full heavy chain of the Mouse anti-MUC1 antibody 139H2 in mammalian cells. Includes a C-terminal -His8 tag for purification.
    Depositor
    Joost Snijder
    Article
    Peng et al Life Sci Alliance. 2024 Mar 20;7(6):e202
    Insert
    anti-MUC1 antibody 139H2 heavy chain (Ighg1 Mouse)
    Use
    Tags
    -AAAHHHHHHHH
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Sept. 26, 2023
    Availability
    Academic Institutions and Nonprofits only
    206201-plasmid-map-sequence-id-408741

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  15. pLKO.1-shGBP1.1.mKO2

    Plasmid
    #85209
    Purpose
    TRCN0000116119 (Target CGACGAAAGGCATGTACCATA) for silencing GBP1 gene and express monomeric Kusabira-Orange2.
    Depositor
    Sandra Martha Gomes Dias
    Article
    Quintero et al BMC Cancer. 2017 Nov 7;17(1):727. doi: 1
    Insert
    GBP1 (guanylate binding protein 1) (GBP1 Human)
    Use
    Lentiviral and RNAi
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    RNA polymerase III promoter for human U6 snRNA fo…
    Available since
    Oct. 4, 2017
    Availability
    Academic Institutions and Nonprofits only
    85209-plasmid-map-sequence-id-169780

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  17. pcDNA3.1_mGFP-FGFR3-G380R

    Plasmid
    #191755
    Purpose
    Expression of N-terminally mGFP labelled mutated FGFR3 (isoform IIIc)-G380R mutation (associated with ACH; target mutation: c.1138G>A; silent mutation added on purpose: c.1131C>T)
    Depositor
    Irene Tiemann-Boege
    Article
    Hartl et al J Biol Chem. 2023 Feb;299(2):102832. doi
    Insert
    mGFP-FGFR3IIIc
    Use
    Tags
    mGFP
    Expression
    Mammalian
    Mutation
    c.1138G>A (G380R) + c.1131C>T (silent mutat…
    Promoter
    Available since
    Jan. 19, 2023
    Availability
    Academic Institutions and Nonprofits only
    191755-plasmid-map-sequence-id-382281

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  18. pNUT N6His K206E/K534A hTFNG

    Plasmid
    #70133
    Purpose
    Expresses N-His tagged nonglycosylated human serum transferrin unable to release iron from the N-lobe or the C-lobe
    Depositor
    Anne Mason, Ross MacGillivray
    Article
    Halbrooks et al Biochemistry. 2003 Apr 8;42(13):3701-7.
    Insert
    N6His K206e/K534A hTFNG (TF Human)
    Use
    Tags
    N-terminal signal peptide, 4 aa link, 6 His, Fact…
    Expression
    Mammalian
    Mutation
    changed Lys206 to a glutamic acid and Lys534 to a…
    Promoter
    SV40
    Available since
    Nov. 10, 2015
    Availability
    Academic Institutions and Nonprofits only
    70133-plasmid-map-sequence-id-129418

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  20. pMT-myl7-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80060
    Purpose
    Myl7 promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Myl7 promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80060-plasmid-map-sequence-id-152594

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  21. pAAV-CreLite

    Plasmid
    #131785
    Purpose
    AAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoter
    Depositor
    George Eisenhoffer
    Article
    Yen et al Dev Dyn. 2020 Nov;249(11):1394-1403. doi
    Insert
    PhyBCreC-P2A-PIF6CreN
    Use
    AAV, Cre/Lox, and Synthetic Biology
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CBh
    Available since
    Jan. 9, 2020
    Availability
    Academic Institutions and Nonprofits only
    131785-plasmid-map-sequence-id-268070

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  23. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

    Plasmid
    #123339
    Purpose
    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.
    Depositor
    Jin Zhang
    Article
    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10
    Insert
    Venus-cpVenus-FLARE-EKAR-EV
    Use
    Tags
    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 28, 2020
    Availability
    Academic Institutions and Nonprofits only
    123339-plasmid-map-sequence-id-307068

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  24. K15-TK/pGL3

    Plasmid
    #44267
    Depositor
    George Cotsarelis
    Article
    Ito et al Nat Med. 2005 Dec;11(12):1351-4. Epub 20
    Insert
    K15 promoter (-4.8) (Krt15 Mouse)
    Use
    Mouse Targeting; Thymidine kinase
    Tags
    HSV-1 TK
    Expression
    Mammalian
    Mutation
    Contains the murine K15 promoter (-4.8) fragment
    Promoter
    murine K15 promoter (-4.8) fragment
    Available since
    April 2, 2013
    Availability
    Academic Institutions and Nonprofits only

  25. pPKm-230

    Plasmid
    #90499
    Purpose
    pSIN - EF-1alpha - PIF3 - MTAD - IRES - PhyB - GBD, dual vector of PIF3-MTAD under EF-1 alpha promoter and PhyB-DBD under IRES promoter; See growth conditions below.
    Depositor
    Todd Coleman
    Article
    Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717
    Insert
    mito-tFD and mito-tFNR
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    Promoter
    Available since
    June 8, 2018
    Availability
    Academic Institutions and Nonprofits only
    90499-plasmid-map-sequence-id-212347

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  26. pMT-zic2a-cytoBirA-2A-mCherry_Ras

    Plasmid
    #80064
    Purpose
    Zic2a promoter driving HA-tagged biotin ligase (BirA), a Thosea asigna virus peptide (2A), and membrane-tethered mCherry protein (membCherry); flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Zebrafish biotagging
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Zic2a enhancer driving c-fos promoter
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    80064-plasmid-map-sequence-id-152615

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  30. pMT-ubb-NLS-BirA-2a-mCherry

    Plasmid
    #79886
    Purpose
    Ubiquitin promoter driving HA-tagged biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein; flanked by Tol2 sequences
    Depositor
    Tatjana Sauka-Spengler
    Article
    Trinh et al Cell Rep. 2017 Apr 11;19(2):425-440. doi
    Insert
    Biotin ligase (BirA) with nuclear localization signal (NLS), a Thosea asigna virus peptide (2A), and mCherry protein
    Use
    Tags
    3x HA
    Expression
    Bacterial
    Mutation
    Promoter
    Ubiquitin
    Available since
    Oct. 11, 2016
    Availability
    Academic Institutions and Nonprofits only
    79886-plasmid-map-sequence-id-150062

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Showing: 5761 - 5790 of 78817 results