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Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

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Showing: 9601 - 9650 of 21628 results

  7. AA332

    Plasmid
    #216053
    Purpose
    Fragmid fragment: (C' terminus) 3x NLS sequences
    Depositor
    John Doench
    Article
    McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi
    Has Service
    Cloning Grade DNA
    Insert
    SV40NLS_v1.10; SV40NLS_v1.11; SV40NLS_v1.12
    Use
    Fragment
    Tags
    Expression
    Mutation
    Promoter
    Available since
    April 29, 2024
    Availability
    Academic Institutions and Nonprofits only
    216053-plasmid-map-sequence-id-431398

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  8. pJRH029

    Plasmid
    #171060
    Purpose
    Plasmid for expressing Gag fused to spyCas9-3x FLAG-2x SV40 NLS with the SQNYPIVQ HIV-1 cleavage site in between
    Depositor
    Jennifer Doudna
    Article
    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:
    Insert
    Gag-spyCas9
    Use
    CRISPR and Lentiviral
    Tags
    2x SV40 NLS and 3x FLAG tag
    Expression
    Mammalian
    Mutation
    Promoter
    CAG
    Available since
    June 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    171060-plasmid-map-sequence-id-457342

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  17. pSCM001

    Plasmid
    #124605
    Purpose
    Expresses mCherry-pHluorin fusion protein in E. coli, a fluorescent reporter for cytoplasmic pH
    Depositor
    David Summers
    Article
    Zarkan et al Sci Rep. 2019 Mar 7;9(1):3868. doi: 10.1
    Insert
    mCherry-pHluorin
    Use
    Tags
    mCherry-(Gly-Gly-Ser)x2-pHluorin translational fu…
    Expression
    Bacterial
    Mutation
    Promoter
    pDAB
    Available since
    May 14, 2019
    Availability
    Academic Institutions and Nonprofits only
    124605-plasmid-map-sequence-id-242761

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  30. pMAH-POLY-eRF1(E55D)

    Plasmid
    #157925
    Purpose
    Encodes a polyspecific aminoacyl-tRNA synthetase fused to a modified releasing factor and four copies of amber suppression tRNAs for unnatural amino acid incorporation in mammalian cells
    Depositor
    Huiwang Ai
    Article
    Chen et al Cell Chem Biol. 2021 Jan 27. pii: S2451-
    Insert
    POLY-eRF1(E55D), Polyspecific aminoacyl-tRNA Synthetase fused to a modified releasing factor through 2A peptide
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    Jan. 8, 2021
    Availability
    Academic Institutions and Nonprofits only
    157925-plasmid-map-sequence-id-310653

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  37. ZJOM65

    Plasmid
    #133654
    Purpose
    Integration of VPH1-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast vacuole.
    Depositor
    Zhiping Xie
    Article
    Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-
    Insert
    p1k-VPH1-mCherry (VPH1 Budding Yeast)
    Use
    Tags
    mCherry
    Expression
    Yeast
    Mutation
    Promoter
    Available since
    Jan. 3, 2020
    Availability
    Academic Institutions and Nonprofits only
    133654-plasmid-map-sequence-id-267529

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  40. pET28a(+)::NL3F10H

    Plasmid
    #141290
    Purpose
    NLUC:3xFlag:10xHis in pET-28 a (+) backbone for bacterial IPTG inducible expression (KmR)
    Depositor
    Andrew Millar
    Article
    Urquiza-Garcia et al Plant Methods. 2019 Jul 8;15:68. doi: 10
    Insert
    NLUC3F10H
    Use
    Luciferase
    Tags
    Flag (x3) and His (x10)
    Expression
    Bacterial
    Mutation
    Engineered for high stability (t1/2 = 11.5 days a…
    Promoter
    promoter for bacteriophage T7 RNA polymerase
    Available since
    Feb. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    141290-plasmid-map-sequence-id-324909

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  44. pTiGc

    Plasmid
    #78314
    Purpose
    Mycobacterial dual reporter plasmid for fluorescence dilution. Description: hsp60-gfp, hsp60(ribo)-turboFP635 (inducible TurboFP635 under control of theophylline - inducible riboswitch).
    Depositor
    Samantha Sampson
    Article
    Mouton et al Microbiology. 2016 Mar 30. doi: 10.1099/
    Inserts
    TurboFP635
    GFP
    Use
    Synthetic Biology; Mycobacterial replicating vect…
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    hsp60 and hsp60(ribo)
    Available since
    July 6, 2016
    Availability
    Academic Institutions and Nonprofits only

  45. pcDNA3-mNeptune2-N

    Plasmid
    #41645
    Purpose
    Expresses far-red FP mNeptune2 fused to Dronpa145N. Dronpa145N oligomerizes under violet light (390-400nm) and dissociates under cyan light (490-500nm)
    Depositor
    Michael Lin
    Article
    Zhou et al Science. 2012 Nov 9;338(6108):810-4. doi
    Insert
    mNeptune2-Dronpa145N
    Use
    Tags
    Expression
    Mammalian
    Mutation
    K145N on Dronpa (allows control of photochromic F…
    Promoter
    CMV
    Available since
    Dec. 21, 2012
    Availability
    Academic Institutions and Nonprofits only
    41645-plasmid-map-sequence-id-296256

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  50. pVDL9.3

    Plasmid
    #168299
    Purpose
    Secretion of polypeptides (nanobodies) in E. coli fused to C-terminal secretion signal of HlyA
    Depositor
    Víctor de Lorenzo
    Article
    Tzschaschel et al Nat Biotechnol. 1996 Jun;14(6):765-9. do
    Insert
    Segment of the hly operon, spanning the C-terminal part of hlyA, hlyB, and hlyD
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    LacI-Plac
    Available since
    June 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    168299-plasmid-map-sequence-id-335286

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Showing: 9601 - 9650 of 21628 results