We narrowed to 4,420 results for: 424

1. pTR-457 tNGFR-P2A-STAT1

   Plasmid

   #186059

   Purpose

   Knockin of truncated NGFR to target gene

   Depositor

   Alexander Marson

   Article

   Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

   Insert

   STAT1 (STAT1 Human)
   

   Use

   CRISPR

   Mutation

   WT with tNGFR sequence

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLEX307_CR-PDHA2S291D

   Plasmid

   #115200

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2S291D (PDHA2 Human)
   

   Use

   Lentiviral

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX307_CR-PDHA2S293D

   Plasmid

   #115201

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2S293D (PDHA2 Human)
   

   Use

   Lentiviral

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLEX307_CR-PDHA2S291D/S293D

   Plasmid

   #115202

   Purpose

   Lentiviral transduction and expression of CRISPR/Cas9-resistant PDHA2S291D/S293D into any mammalian cell (when using sgRNA 5'-ATGTAATGACGTGATCCGAG-3')

   Depositor

   Mohan Babu

   Article

   Jessulat et al Nucleic Acids Res. 2021 Jul 9. pii: 6318

   Insert

   CR (CRISPR/Cas9-resistant)-PDHA2S291D/S293D (PDHA2 Human)
   

   Use

   Lentiviral

   Mutation

   c.321C>T (silent when translated to protein, c…

   Promoter

   EF1alpha

   Available Since

   July 5, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pUG36-NES-EGFP-cPHx3

   Plasmid

   #183668

   Purpose

   PtdIns(3,4)P2 biosensor for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Frog, Human)
   

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Yeast

   Mutation

   Amino acids 169-329 (tandem trimer)

   Promoter

   MET25

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUG34-NES-EGFP-cPHx3

   Plasmid

   #183669

   Purpose

   PtdIns(3,4)P2 biosensor for expression in yeast

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   PLEKHA1 (PLEKHA1 Frog, Human)
   

   Tags

   X. laevis map2k1.L(32-44)-EGFP

   Expression

   Yeast

   Mutation

   Amino acids 169-329 (tandem trimer)

   Promoter

   MET25

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. NES-mCherry-PH-BTKx1

   Plasmid

   #183653

   Purpose

   PtdIns(3,4,5)P3 biosensor

   Depositor

   Sergio Grinstein

   Article

   Walpole et al Nat Cell Biol. 2022 Apr 28. pii: 10.1038

   Insert

   BTK (BTK Frog, Human)
   

   Tags

   X. laevis map2k1.L(32-44)-mCherry

   Expression

   Mammalian

   Mutation

   Amino acids 2-170

   Promoter

   CMV

   Available Since

   May 3, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. A1-LCD-30G+30S-12F+12Y

   Plasmid

   #178687

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   A1-LCD-30G+30S-12F+12Y (HNRNPA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   thirty Gly residues replaced with Ser, twelve Phe…

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. A1-LCD-30G+30S+7F-7Y

   Plasmid

   #178686

   Purpose

   Bacterial expression of N-terminally 6His tagged A1-LCD with thirty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)

   Depositor

   Tanja Mittag

   Article

   Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

   Insert

   A1-LCD-30G+30S+7F-7Y (HNRNPA1 Human)
   

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   thirty Gly residues replaced with Ser, seven Tyr …

   Available Since

   March 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR-SV40-BTLAecto&tmd-PD-1icd-mGFP

    Plasmid

    #180795

    Purpose

    Expression of human BTLA extracellular domain and transmembrane domain fused to PD-1 intracellular domain followed by mEGFP

    Depositor

    Enfu Hui

    Article

    Xu et al J Cell Biol. 2020 Jun 1;219(6). pii: 151

    Insert

    BTLAecto&tm-PD-1int (BTLA, PDCD1 Human)
    

    Use

    Lentiviral

    Tags

    mEGFP

    Promoter

    SV40

    Available Since

    March 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. A1-LCD-20G+20S+7F-7Y

    Plasmid

    #178688

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, seven Tyr residues replaced with Phe (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-20G+20S+7F-7Y (HNRNPA1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    twenty Gly residues replaced with Ser, seven Tyr …

    Available Since

    March 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. A1-LCD-14N-4Q+18G

    Plasmid

    #178694

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with fourteen Gln residues removed, four Asn removed, replaced with eighteen Gly (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-14N-4Q+18G (HNRNPA1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    fourteen Gln residues removed, four Asn removed, …

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. A1-LCD-20G+20S-12F+12Y

    Plasmid

    #178689

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty Gly residues replaced with Ser, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD-20G+20S-12F+12Y (HNRNPA1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    twenty Gly residues replaced with Ser, twelve Phe…

    Available Since

    March 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. A1-LCD+23G-23S-12F+12Y

    Plasmid

    #178691

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, twelve Phe residues replaced with Tyr (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+23G-23S-12F+12Y (HNRNPA1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    twenty-three Ser residues replaced with Gly, twel…

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. A1-LCD+23G-23S+7F-7Y

    Plasmid

    #178690

    Purpose

    Bacterial expression of N-terminally 6His tagged A1-LCD with twenty-three Ser residues replaced with Gly, seven Tyr replaced with Phe (LCD: low-complexity domain, aa 186-320)

    Depositor

    Tanja Mittag

    Article

    Bremer et al Nat Chem. 2022 Feb;14(2):196-207. doi: 1

    Insert

    A1-LCD+23G-23S+7F-7Y (HNRNPA1 Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    twenty-three Ser residues replaced with Gly, seve…

    Available Since

    March 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. dCas9-CBP RING-PHD-HAT

    Plasmid

    #179549

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human CBP RING-PHD-HAT domain (aa 1191-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. dCas9-CBP PHD-HAT

    Plasmid

    #179548

    Purpose

    encodes S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) driven by EF-1alpha promoter

    Depositor

    Isaac Hilton

    Article

    Wang et al Nucleic Acids Res. 2022 Aug 12;50(14):78

    Insert

    S. pyogenes dCas9 with c-terminal fusion of human CBP PHD-HAT domain (aa 1279-1701) (CREBBP Human)
    

    Use

    Lentiviral

    Tags

    Flag Tag

    Expression

    Mammalian

    Mutation

    D10A; H840A in S.pyogenes Cas9

    Promoter

    EF-1alpha

    Available Since

    Feb. 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p3E-BIN1_tv8

    Plasmid

    #126574

    Purpose

    Multisite gateway middle entry vector containing human BIN1 transcriptional variant 8 (muscle specific) with no ATG. Parton lab clone DVA.

    Depositor

    Rob Parton

    Article

    Lo et al J Cell Biol. 2021 Dec 6;220(12). pii: 21

    Insert

    BIN1 (BIN1 Human)
    

    Use

    Gateway entry clone

    Available Since

    Nov. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAB(EXPR-DS-HDAC5_HDAC3)

    Plasmid

    #114399

    Purpose

    For PYL-HDAC5 catalytic domain swap to HDAC3 catalytic domain expression

    Depositor

    Vincent Dion

    Article

    Yang et al Hum Mol Genet. 2021 Sep 7. pii: 6365982.

    Insert

    PYL-HDAC5_HDAC3 catalytic domain swap (HDAC3, HDAC5 Human)
    

    Tags

    3xFlag-NLS (internal)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pnCS_DSep2_Peanut-TEV-Strep

    Plasmid

    #174495

    Purpose

    bacterial co-expression of Drosophila Sep2 and of Drosophila Peanut

    Depositor

    Manos Mavrakis

    Article

    Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

    Inserts

    Sep2 (Sep2 Fly)
    peanut (pnut Fly)
    

    Tags

    TEV-Strep

    Expression

    Bacterial

    Available Since

    Sept. 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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