We narrowed to 5,494 results for: SPI

1. DR5rev::erRFP

   Plasmid

   #61011

   Purpose

   Auxin signalling reporter (DR5rev) driving RFP (ER-targeted)

   Depositor

   Alexis Maizel

   Article

   Marin et al Plant Cell. 2010 Apr;22(4):1104-17. doi:

   Insert

   DR5rev::erRFP:35S_term
   

   Use

   Tags

   Expression

   Plant

   Mutation

   Promoter

   DR5rev::erRFP:35Sterm

   Available since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCI-SEP-GluR2(Q)

   Plasmid

   #24002

   Depositor

   Robert Malinow

   Article

   Kopec et al J Neurosci. 2006 Feb 15. 26(7):2000-9.

   Insert

   GluR2 (Gria2 Rat)
   

   Use

   Tags

   GluR2 signal peptide (aa1-24) and SEP (supereclip…

   Expression

   Mammalian

   Mutation

   R607Q

   Promoter

   CMV

   Available since

   April 6, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCSN075

   Plasmid

   #166732

   Purpose

   Single copy yeast plasmid expressing DNase-dead dLbCas12a E925A fused to NLS and Mxi, codon optimized for Saccharomyces cerevisiae

   Depositor

   Rene Verwaal

   Article

   Ciurkot et al Nucleic Acids Res. 2021 Jul 1. pii: 6312

   Insert

   dCas12a-NLS-Mxi1
   

   Use

   CRISPR

   Tags

   Mxi1 and SV40 NLS

   Expression

   Yeast

   Mutation

   E925A

   Promoter

   S. cerevisiae TEF1

   Available since

   June 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-SARS-CoV-2-HiBiT-N

   Plasmid

   #215815

   Purpose

   Expresses N protein with HiBiT tag in mammalian cells

   Depositor

   Thomas Gallagher

   Article

   Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

   Insert

   SARS-CoV-2 HiBiT Nucleocapsid (N )
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28_human-pro-IL18

   Plasmid

   #183390

   Purpose

   Overexpression of catalytic domain of inflammatory caspase in bacteria

   Depositor

   Jonathan Kagan

   Article

   Devant et al Sci Immunol. 2021 Aug 10;6(62). doi: 10.

   Insert

   pro-IL18 (IL18 Human)
   

   Use

   Tags

   His-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBS U6a gRNA

   Plasmid

   #158961

   Purpose

   gRNA backbone driven by zebrafish U6a promoter

   Depositor

   Michael Brand

   Article

   Hans et al Nat Commun. 2021 Feb 18;12(1):1125. doi:

   Inserts

   zf U6a promoter
   gRNA backbone
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Feb. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCI SMN1

   Plasmid

   #72286

   Purpose

   SMN1 exon 7 splicing cassette

   Depositor

   Elliot Androphy

   Article

   Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

   Insert

   SMN1 (SMN1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   exons 6-7-8 including introns

   Promoter

   CMV

   Available since

   Jan. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

8. pAAV-EF1a-DIO-tdTomato-WPRE

   Plasmid

   #133786

   Purpose

   Cre-dependent tdTomato fluorescent reporter for cell labeling

   Depositor

   Michael Stryker

   Article

   Sun et al Proc Natl Acad Sci U S A. 2019 Oct 22;11

   Insert

   pAAV-EF1a-DIO-tdTomato-WPRE
   

   Use

   AAV and Cre/Lox

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. LbCas12a-nuCPE

   Plasmid

   #213748

   Purpose

   For circular RNA-mediated prime editor using LbCas12a-nuCPE in HEK293T cells

   Depositor

   Caixia Gao

   Article

   Liang et al Nat Biotechnol. 2024 Jan 10. doi: 10.103

   Insert

   MCP-LbCas12a-M-MLV RT∆RNase H
   

   Use

   CRISPR

   Tags

   BPNLS

   Expression

   Mammalian

   Mutation

   D156R in LbCas12a

   Promoter

   CMV

   Available since

   Feb. 7, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pBSV2G_P0826-BB0323-mCherryBb

    Plasmid

    #118244

    Purpose

    Kanamycin-resistant E.coli/B. burgdorferi shuttle vector; Encodes a BB0323-mCherry translational fusion under the control of the bb0826 promoter

    Depositor

    Christine Jacobs-Wagner

    Article

    Takacs et al Appl Environ Microbiol. 2018 Oct 12. pii

    Insert

    BB0323-mCherry translational fusion under the control of the bb0826 promoter
    

    Use

    Tags

    mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Dec. 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV-dCpf1-BE

    Plasmid

    #107685

    Purpose

    Expresses dLbCpf1-BE in mammalian cells

    Depositor

    Jia Chen

    Article

    Li et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

    Insert

    dLbCpf1-BE
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    D832A/E925A/D1148A

    Promoter

    CMV

    Available since

    April 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. ChR2-2A-Halo

    Plasmid

    #22047

    Purpose

    Expression of ChR2-GFP and Halo-YFP for bi-directional control of neuronal activity

    Depositor

    Edward Boyden

    Article

    Han et al Front Mol Neurosci. 2009 Aug 27:2:12. do

    Insert

    ChR2-GFP-2A-Halo-YFP
    

    Use

    Tags

    GFP and YFP

    Expression

    Mammalian

    Mutation

    Note that there is a S147R mut in YFP. This mutat…

    Promoter

    CMV

    Available since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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13. PTEF1-yEGFPCLN2PEST-pRS406

    Plasmid

    #64406

    Purpose

    TEF promoter driven GFP expression

    Depositor

    Claudia Vickers

    Article

    Williams et al Microb Cell Fact. 2015 Apr 1;14:43. doi:

    Insert

    TEF1 promoter (TEF1 Budding Yeast)
    

    Use

    Tags

    yEGFPCLN2PEST (destabilized yeast EGFP)

    Expression

    Yeast

    Mutation

    Promoter

    TEF1 promoter

    Available since

    May 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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14. pJS183

    Plasmid

    #134142

    Purpose

    Bacterial expression of GFP-Ska1 and Ska2

    Depositor

    Iain Cheeseman

    Article

    Monda et al Curr Biol. 2017 Dec 4;27(23):3666-3675.e

    Insert

    His-GFP-human Ska1; human Ska2
    

    Use

    Tags

    GFP

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAT005

    Plasmid

    #171632

    Purpose

    2nd generation lentiviral transfer plasmid encoding a U6 promoter expressing a spyCas9 sgRNA targeting B2M and an EF1-a promoter expressing mNeonGreen

    Depositor

    Jennifer Doudna

    Article

    Hamilton et al Cell Rep. 2021 Jun 1;35(9):109207. doi:

    Inserts

    spyCas9 sgRNA-B2M
    mNeon
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1-a and U6

    Available since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA3.1-SARS-CoV-2-E

    Plasmid

    #215814

    Purpose

    Expresses SARS-CoV-2 Envelope protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Envelope (E )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3.1-SARS-CoV-2-M

    Plasmid

    #215813

    Purpose

    Expresses SARS-CoV-2 Membrane protein in mammalian cells

    Depositor

    Thomas Gallagher

    Article

    Qing et al mBio. 2021 Aug 31;12(4):e0159021. doi: 1

    Insert

    SARS-CoV-2 Membrane (M )
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 20, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. FEMP

    Plasmid

    #191973

    Purpose

    FRET-based indicator of ER-mitochondria proximity (FEMP)

    Depositor

    Luca Scorrano

    Article

    Naon et al Proc Natl Acad Sci U S A. 2016 Oct 4;113

    Insert

    AkapYFP-Tav2a-CFP-sac1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pcDNA3.1-hLbCpf1(TYCV) (pY230)

    Plasmid

    #89355

    Purpose

    Expresses humanized LbCpf1 variant that recognizes TYCV PAMs

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1, RR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    G532R/K595R

    Promoter

    CMV

    Available since

    June 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pEK36

    Plasmid

    #210598

    Purpose

    Reporter gene expression in B. dendrobatidis

    Depositor

    Lillian Fritz-Laylin

    Article

    Kalinka et al Proc Natl Acad Sci U S A. 2024 Jan 23;12

    Insert

    SpH2Bpro-Hshph-G4S-HsmClover3-ScADH1ter
    

    Use

    Tags

    mClover3

    Expression

    Bacterial and Yeast

    Mutation

    Promoter

    SpH2Bpro

    Available since

    Jan. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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