We narrowed to 23,150 results for: CRISPR

1. RFN_EGFP_80

   Plasmid

   #60072

   Purpose

   Expresses a pair of control multiplex gRNAs targeting EGFP for use with dimeric hFokI-dCas9 nuclease

   Depositor

   Keith Joung

   Article

   Tsai et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   pair of multiplex gRNAs targeting EGFP
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Oct. 16, 2014

   Availability

   Academic Institutions and Nonprofits only

2. pSpCas9(BB)-2A-Blast

   Plasmid

   #118055

   Purpose

   Used for expression of sgRNA in mammalian cells

   Depositor

   Ken-Ichi Takemaru

   Type

   Empty backbone

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Dec. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. lenti-RfxCas13d-Blast-BFP

   Plasmid

   #196726

   Purpose

   Sortable and selectable RfxCas13d.

   Depositor

   Bernhard Schmierer

   Insert

   RfxCas13d-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX551-miniCMV-SpCas9

   Plasmid

   #107031

   Purpose

   AAV plasmid expressing Cas9 under control of miniCMV promoter

   Depositor

   Alex Hewitt

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti.Cas9.BFP.Blast

   Plasmid

   #196714

   Purpose

   WT SpCas9 with BSD-TagBFP for selection and sorting

   Depositor

   Bernhard Schmierer

   Insert

   Cas9-T2A-BSD-TagBFP
   

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. CROPSeq-PP7-p65-HSF

   Plasmid

   #196719

   Purpose

   CROP-Seq library plasmid for CRISPR-A with SAM system (p65-HSF1 activator recruited to PP7 binding loops in the tracrRNA of the guide).

   Depositor

   Bernhard Schmierer

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Nucleoplasmin NLS and SV40 NLS

   Expression

   Mutation

   Promoter

   Available Since

   April 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pPB-CAG-dCas9-VPR-wpA-pPuroTK

   Plasmid

   #214127

   Purpose

   Gene expression by Piggybac transposase in mammalian cells

   Depositor

   Sho Yoshimatsu

   Insert

   dCas9-VPR
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Codon-optimized for mammalian cells

   Promoter

   Available Since

   Feb. 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. eSpCas9(1.1)-T2A-Puro

   Plasmid

   #101039

   Purpose

   Enhanced SpCas9(1.1) with puromycin resistance gene via T2A linker.

   Depositor

   Andrea Németh

   Type

   Empty backbone

   Use

   CRISPR

   Tags

   3xFLAG and T2A-Puro

   Expression

   Mammalian

   Mutation

   Promoter

   CBh

   Available Since

   Nov. 1, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pTRE3G-VP64-dCas9-VP64-ZsG

   Plasmid

   #192668

   Purpose

   Dox-inducible expression of VP64-dCas9-VP64 with 2A ZsGreen1

   Depositor

   Jeffrey Miner

   Insert

   VP64-dCas9-VP64, ZsGreen1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   D10A and N863A in Cas9

   Promoter

   TRE3G

   Available Since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. pHR Ef1a-dCas9-BFP-KRAB

    Plasmid

    #217304

    Purpose

    lentiviral vector for dCas9-BFP-KRAB on Ef1alpha promoter

    Depositor

    Luke Gilbert

    Insert

    dCas9-tagBFP-KRAB
    

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    Expression

    Mutation

    Promoter

    Ef1alpha

    Available Since

    April 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX551-CMV-CjCas9

    Plasmid

    #107035

    Purpose

    AAV plasmid expressing CjCas9 under control of CMV promoter

    Depositor

    Alex Hewitt

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pY130 (pcDNA3.1-U6-huBsCpf1)

    Plasmid

    #92306

    Purpose

    Expression of humanized BsCpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huBsCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pX551-miniCMV-AsCpf1

    Plasmid

    #107034

    Purpose

    AAV plasmid expressing AsCpf1 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    AsCpf1
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pY128 (pcDNA3.1-U6-huMb2Cpf1)

    Plasmid

    #92304

    Purpose

    Expression of humanized Mb2Cpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huMb2Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pY129 (pcDNA3.1-U6-huMb3Cpf1)

    Plasmid

    #92305

    Purpose

    Expression of humanized Mb3Cpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huMb3Cpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pY127 (pcDNA3.1-U6-huTsCpf1)

    Plasmid

    #92303

    Purpose

    Expression of humanized TsCpf1 and crRNA

    Depositor

    Feng Zhang

    Insert

    huTsCpf1
    

    Use

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX551-miniCMV-CjCas9

    Plasmid

    #107033

    Purpose

    AAV plasmid expressing CjCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    CjCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. pX551-miniCMV-SaCas9

    Plasmid

    #107032

    Purpose

    AAV plasmid expressing SaCas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    SaCas9
    

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pRR2

    Plasmid

    #166097

    Purpose

    Gal inducible expression of dCas9-FokI

    Depositor

    Rebecca Burgess

    Insert

    dCas9-FokI
    

    Use

    CRISPR

    Tags

    Expression

    Yeast

    Mutation

    spCas9-D10A, H840A

    Promoter

    Gal-inducible

    Available Since

    March 31, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV-PE2-pegRNA-tetra-com vector

    Plasmid

    #136271

    Purpose

    Expresses PE2 and Tetra-com modified pegRNA cassette

    Depositor

    Baisong Lu

    Insert

    PE2 and pegRNA cassette
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 23, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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