We narrowed to 22,382 results for: Sis

1. B52_puro_gRNA_3418711_KCNB2

   Plasmid

   #197558

   Purpose

   gRNA targeting upstream region of KCNB2 (site 3418711, control for epigenetic targeting)

   Depositor

   Darius Bägli

   Insert

   upstream KCNB2 promoter gRNA (Kcnb2 Rat)
   

   Use

   Grna with puromycin selection

   Tags

   puromycin resistance cassette under CMV promotion

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   March 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. Coexpressed Free NES

   Plasmid

   #182482

   Purpose

   Yeast integrative plasmid for expressing ERG20 (GAL10 promoter) and nerolidol synthase from Actinidia chinensis (AcNES1; GAL7 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

   Depositor

   Claudia Vickers

   Article

   Cheah et al Metab Eng. 2023 May;77:143-151. doi: 10.

   Inserts

   FPPS
   NES
   

   Use

   Synthetic Biology; Metabolic engineering

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   GAL10 and GAL7

   Available since

   Nov. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pNOC_dCas9_sgRNA Td2

   Plasmid

   #176258

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged with Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   dead spCas9
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   H840A and D10A mutations on spCas9 to inactivate …

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pNOC_dfnCas12a_crRNA Td2

   Plasmid

   #176255

   Purpose

   pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged with Nlux and crRNA targeting the fluorescent gene tdtomato with spacer 2

   Depositor

   John van der Oost

   Article

   Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

   Insert

   humanized fnCas12a
   

   Use

   CRISPR, Luciferase, and Synthetic Biology ; Expre…

   Tags

   luciferase

   Expression

   Mutation

   D917A and E1006A mutations to inactivate the endo…

   Promoter

   Available since

   Nov. 16, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pENTR/D-Topo genomic A. thaliana IMPORTIN ALPHA 4

   Plasmid

   #175815

   Purpose

   pENTR plasmid with genomic sequence of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 4 (AT1G09270) without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller, Mark Banfield

   Article

   Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

   Insert

   IMPORTIN ALPHA ISOFORM 4 (IMPA-4 Mustard Weed)
   

   Use

   Pentr plasmid for gateway cloning

   Tags

   none

   Expression

   Mutation

   Promoter

   none

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 2

   Plasmid

   #175816

   Purpose

   pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 2 (AT4G16143.1) without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller, Mark Banfield

   Article

   Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

   Insert

   IMPORTIN ALPHA ISOFORM 2 (IMPA-2 Mustard Weed)
   

   Use

   Pentr plasmid for gateway cloning

   Tags

   none

   Expression

   Mutation

   natural polymorphism P65S, annotated in Genbank f…

   Promoter

   none

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pENTR/D-Topo CDS A. thaliana IMPORTIN ALPHA 9

   Plasmid

   #175817

   Purpose

   pENTR plasmid with CDS of Arabidopsis thaliana IMPORTIN ALPHA ISOFORM 9 (AT5G03070.1) without stop codon for C-terminal epitope tags

   Depositor

   Lennart Wirthmueller, Mark Banfield

   Article

   Wirthmueller et al Plant J. 2015 Jan;81(1):40-52. doi: 10.1

   Insert

   IMPORTIN ALPHA ISOFORM 9 (IMPA-9 Mustard Weed)
   

   Use

   Pentr plasmid for gateway cloning

   Tags

   none

   Expression

   Mutation

   Promoter

   none

   Available since

   Oct. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a-ybbr-ELP-ddFLN4-XMod-Doc-HIS (BMB-KO)

   Plasmid

   #153441

   Purpose

   E. coli expression of Rc. XDocB binding mode B knock-out construct containing ybbr tag, ELP linker and ddFLN4 fingerprint domain. This construct was designed for AFM measurements.

   Depositor

   Michael Nash

   Article

   Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

   Insert

   Rc. XDocB binding mode B knock-out mutant
   

   Use

   Tags

   3xELP linker, 6xHis, ddFLN4 fingerprint domain, a…

   Expression

   Bacterial

   Mutation

   Arginine 140 and methionine 144 were mutated to a…

   Promoter

   T7 promoter

   Available since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMXS-IRES-Blast ACSL4

   Plasmid

   #173164

   Purpose

   Retroviral vector to express sgRNA resistant human ACSL4

   Depositor

   Kivanc Birsoy

   Article

   Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

   Insert

   Acyl-CoA Synthetase Long Chain Family Member 4 (ACSL4 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Synonymous mutations at sgRNA sites

   Promoter

   MMLV

   Available since

   Aug. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMXS-IRES-Blast HA-GPAT4

    Plasmid

    #173168

    Purpose

    Retroviral vector to express sgRNA resistant HA tagged human GPAT4

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Mol Cell. 2019 Apr 4;74(1):45-58.e7. doi

    Insert

    Glycerol-3-Phosphate Acyltransferase 4 (GPAT4 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mutation

    Synonymous mutations at sgRNA sites

    Promoter

    MMLV

    Available since

    Aug. 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lenti-RNF2-ngRNA+41_EF1a-puroR

    Plasmid

    #207358

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 +41 ngRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAGGS-PHFF-sh-hBVRA

    Plasmid

    #100285

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    

    Use

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCROPseq-VEX-optimized-scaffold

    Plasmid

    #203321

    Purpose

    Modified CROPseq vector for efficient CRISPR screens in hematopoiesis

    Depositor

    Vijay Sankaran

    Article

    Martin-Rufino et al Cell. 2023 Apr 27:S0092-8674(23)00332-X.

    Insert

    sgRNA
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNOC_dfnCas12a-KRAB_crRNA NC

    Plasmid

    #176262

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized fnCas12a gene sequence tagged to the KRAB domain and Nlux and spacer sequence is replaced by the type IIS restriction site for endonucleas

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    humanized fnCas12a
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Expression

    Mutation

    D917A and E1006A mutations to inactivate the endo…

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pNOC_dCas9-KRAB_sgRNA Td2

    Plasmid

    #176264

    Purpose

    pNOC episomal plasmid harboring the dead version of humanized spCas9 gene sequence tagged to the KRAB domain and Nlux and sgRNA targeting the fluorescent gene tdtomato with spacer 2

    Depositor

    John van der Oost

    Article

    Naduthodi et al ACS Synth Biol. 2021 Dec 17;10(12):3369-

    Insert

    dead spCas9
    

    Use

    CRISPR, Luciferase, and Synthetic Biology ; Expre…

    Tags

    Kruppel associated box domain and luciferase

    Expression

    Mutation

    H840A and D10A mutations on spCas9 to inactivate …

    Promoter

    Available since

    Nov. 16, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUC19-Top2A-5X Gly-Venus-T2A-Hygro

    Plasmid

    #211115

    Purpose

    For tagging the C-term of Top2A with Venus

    Depositor

    Job Dekker

    Article

    Hildebrand et al Mol Cell. 2024 Mar 13:S1097-2765(24)0014

    Inserts

    5' C-term Top2A homology arm (TOP2A Human)
    Venus
    T2A
    Blastocidin Resistance
    5' C-term Top2A homology arm (TOP2A Human)
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    n/a

    Available since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pcDNA3-N-HA-NEK7 K64M

    Plasmid

    #75143

    Purpose

    Expresses N-terminal HA-tagged NEK7 (with a K64M substitution; kinase dead) in mammalian cells

    Depositor

    Bruce Beutler

    Article

    Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

    Insert

    NIMA (never in mitosis gene a)-related expressed kinase 7 (Nek7 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Lys 64 mutated to Met; kinase inactive and F236L …

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. hEF1α-H2B-mOrange2-IRES-EGFP PGK-Puromycin

    Plasmid

    #99277

    Purpose

    Dual fluorescent reporter - constitutive expression of mOrange2 (549/565) localized in the nucleus, EGFP (488/507) untargeted and visualized in the cytoplasm, and Puromycin resistance

    Depositor

    Jordan Miller

    Article

    Calderon et al Biomater Sci. 2017 Jun 29. doi: 10.1039/

    Inserts

    H2B-mOrange2-IRES-EGFP
    Puro
    

    Use

    Lentiviral

    Tags

    EGFP, H2B, IRES, PGK, and mOrange2

    Expression

    Mutation

    Promoter

    EF1 alpha-human and PGK-mouse

    Available since

    Nov. 13, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCW-GemininΔDbox-HA-Puro

    Plasmid

    #193770

    Purpose

    Doxycyline-inducible (TetOn) human Geminin (C-terminal HA tag) with Dbox removed to prevent degradation by APC/C, expressed from all-in-one pCW vector (TetOn + rtTA)

    Depositor

    Tobias Meyer

    Article

    Ratnayeke et al Mol Cell. 2023 Jan 5;83(1):26-42.e13. do

    Insert

    GemininΔDbox-HA (GMNN Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mutation

    Dbox removed (R23A, L26A). Codons switched at siR…

    Promoter

    TRE

    Available since

    Jan. 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMDC32B-AtTAS1c-D2-B/c-AtMIR173

    Plasmid

    #137885

    Purpose

    Plant expression vector for direct cloning of synthetic trans-acting siRNAs into Arabidopsis thaliana TAS1c precursor downstream 3'D1[+]. Contains AtMIR173 for syntasi expression in any plant species.

    Depositor

    Alberto Carbonell

    Article

    Lopez-Dolz et al Nucleic Acids Res. 2020 May 12. pii: 583

    Inserts

    AtTAS1c-D2-B/c
    2x35S-AtMIR173-Tnos
    

    Use

    Tags

    Expression

    Plant

    Mutation

    A. thaliana TAS1c precursor sequence including a …

    Promoter

    Available since

    May 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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