We narrowed to 11,766 results for: nsf

1. pAAV-UbC-mEmerald Rab7a

   Plasmid

   #115238

   Purpose

   To express in mammalian cells the endocytic protein Rab7a fused to mEmerald by transfection or by generating AAV virions.

   Depositor

   Pantelis Tsoulfas

   Insert

   Rab7a fused to mEmerald (RAB7A Human)
   

   Use

   AAV

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   7 Amino Acids (21 Nucleotides) between mEmerald a…

   Promoter

   Human UbC

   Available Since

   Sept. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pmirGLO-3'UTR mouse Il13

   Plasmid

   #207125

   Purpose

   Luciferase vector containing 3'UTR for mouse Il13

   Depositor

   Silvia Monticelli

   Article

   Leoni et al Nat Commun. 2023 Jun 29;14(1):3862. doi:

   Insert

   Interleukin 13 (Il13) 3'UTR (Il13 Mouse)
   

   Use

   Luciferase

   Expression

   Mammalian

   Promoter

   PGK promoter

   Available Since

   Dec. 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GW1-mito-mCherry-roGFP2

   Plasmid

   #107220

   Purpose

   Mammalian expression plasmid to target the mCherry-roGFP2 sensor to the mitochondrial matrix.

   Depositor

   Mathew Tantama

   Article

   Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

   Insert

   mCherry-roGFP2
   

   Tags

   4xCox8

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDEST47-ARL1-GFP

   Plasmid

   #67395

   Purpose

   Mammalian expression of hArl1 with C-term GFP tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL1 (ARL1 Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   bp 231 C to T; silent mutation

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

5. MSCV-human CSFI-R-L301S- Y969F-IRES-GFP

   Plasmid

   #133745

   Purpose

   retroviral expression of CSF1-R mutant

   Depositor

   Martine Roussel

   Article

   Roussel et al Nature. 1987 Feb 5-11;325(6104):549-52.

   Insert

   CSF1-R (CSF1R Human)
   

   Use

   Retroviral

   Expression

   Mammalian

   Mutation

   L301S- Y969F

   Promoter

   MSCV

   Available Since

   Dec. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pGAPDH::H2B-mScarlet

   Plasmid

   #186764

   Purpose

   A plasmid containing planarian H2B fused to a planarian codon-optimized mScarlet, driven by the planarian GAPDH promoter.

   Depositor

   Bo Wang

   Article

   Hall et al Cell Rep Methods. 2022 Sep 20;2(10):1002

   Insert

   H2B-mScarlet
   

   Use

   Overexpression

   Tags

   H2B

   Promoter

   GAPDH

   Available Since

   Oct. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pSUPER-retro-puro-Smad4

   Plasmid

   #89829

   Purpose

   Knockdown human Smad4 expression in human mammalian cells

   Depositor

   Shengyu Yang

   Article

   Sun et al J Biol Chem. 2011 Nov 11;286(45):38865-7

   Insert

   Smad4 shRNA (SMAD4 Mouse, Human)
   

   Use

   Retroviral

   Available Since

   May 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pDSHA-ARL8B-Myc

   Plasmid

   #67362

   Purpose

   Mammalian expression of hArl8b with C-term Myc tag

   Depositor

   Richard Kahn

   Article

   Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

   Insert

   ARL8B (ARL8B Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 5, 2015

   Availability

   Academic Institutions and Nonprofits only

9. GW1-mito-mRuby2-roGFP1

   Plasmid

   #107219

   Purpose

   Mammalian expression plasmid to target the rmRuby1-roGFP1 sensor to the mitochondrial matrix.

   Depositor

   Mathew Tantama

   Article

   Norcross et al ACS Sens. 2017 Nov 22;2(11):1721-1729. d

   Insert

   mRuby2-roGFP1
   

   Tags

   4xCox8

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   March 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pDEST47-ARL8B

    Plasmid

    #67467

    Purpose

    Mammalian expression of hArl8b with stop codon (native expression)

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

11. SpIMPDH

    Plasmid

    #206980

    Purpose

    Streptococcus pyogenes Inosine 5′-Monophosphate Dehydrogenase (SpIMPDH, EC. 1.1.1.205). Contains His6 tag with TEV protease cleavage site in pET-28b(+) plasmid for protein expression.

    Depositor

    Vern Schramm

    Article

    Glockzin et al Biochemistry. 2022 Oct 4;61(19):2088-210

    Insert

    SpIMPDH (guaB Streptococcus pyogenes)
    

    Tags

    His tag (with TEV cleavage site)

    Expression

    Bacterial

    Available Since

    March 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pET-DEST42-ARF6-V5-His6

    Plasmid

    #67415

    Purpose

    Expression of hArf6 with C-term V5 and His6 tag in E. coli

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARF6 (ARF6 Human)
    

    Tags

    His and V5

    Expression

    Bacterial

    Promoter

    T7/lacO

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

13. pDEST47-ARL6-GFP

    Plasmid

    #67401

    Purpose

    Mammalian expression of hArl6 with C-term GFP tag

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL6 (ARL6 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pDONR221-ARL8B

    Plasmid

    #67334

    Purpose

    hArl8b with stop codon in Gateway Entry vector

    Depositor

    Richard Kahn

    Article

    Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

    Insert

    ARL8B (ARL8B Human)
    

    Use

    Gateway entry vector

    Expression

    Bacterial

    Promoter

    None

    Available Since

    Oct. 5, 2015

    Availability

    Academic Institutions and Nonprofits only

15. pD21-hgAPOAI

    Plasmid

    #124962

    Purpose

    Express ApoAI in the liver

    Depositor

    Kazuhiro Oka

    Article

    Belalcazar et al Circulation. 2003 Jun 3;107(21):2726-32.

    Insert

    human APOAI gene (LPA Human)
    

    Use

    Adenoviral

    Promoter

    human APOAI

    Available Since

    July 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. GFP-IP3KC

    Plasmid

    #134603

    Purpose

    Expresses IP3KC GFP tagged

    Depositor

    Christophe Erneux

    Article

    Dewaste et al Biochem J. 2003 Aug 15;374(Pt 1):41-9.

    Insert

    IP3KC (ITPKC Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    Nov. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. ALARM NMR

    Plasmid

    #90209

    Purpose

    Expresses the human La antigen for use in ALARM NMR

    Depositor

    Michael Walters

    Article

    Dahlin et al Nat Commun. 2017 Nov 15;8(1):1527. doi:

    Insert

    La antigen (SSB Human)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    contains amino acids 100-324

    Promoter

    T7 promoter

    Available Since

    Feb. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pCAG-hNme2Cas9-NLS(SV40)-3xFLAG (KAC582)

    Plasmid

    #134331

    Purpose

    CAG promoter expression plasmid for human codon optimized Nme2Cas9 nuclease with C-terminal NLS (SV40) and 3x FLAG tag

    Depositor

    Joseph Bondy-Denomy, Benjamin Kleinstiver

    Article

    Mahendra et al Nat Microbiol. 2020 Apr;5(4):620-629. do

    Insert

    human codon optimized Nme2Cas9
    

    Tags

    NLS(SV40)-3xFLAG

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    March 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. gh136

    Plasmid

    #106806

    Purpose

    expression of gRNA targeting EXT2

    Depositor

    Eric-Paul Bennett

    Article

    Narimatsu et al Glycobiology. 2018 Jan 5. pii: 4791732.

    Insert

    EXT2 (EXT2 Human)
    

    Expression

    Mammalian

    Available Since

    Nov. 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pcDNA3.1-mGnptg

    Plasmid

    #78106

    Purpose

    Mammalian expression of mouse Gnptg with C-terminal His tag

    Depositor

    Thomas Braulke

    Article

    Encarnacao et al J Biol Chem. 2011 Feb 18;286(7):5311-8.

    Insert

    Gnptg (Gnptg Mouse)
    

    Tags

    His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 19, 2016

    Availability

    Academic Institutions and Nonprofits only