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[#GS24 -LV] Syn1-GFP-OMP25

Plasmid

#233566

Purpose

Lentiviral construct of mitochondrial localized GFP to be expressed in the donor cells under the Synapsin1 promotor

Depositor

Simon Grelet

Publication

Hoover et al Nature. 2025 Jun 25. doi: 10.1038/s41586

Insert

eGFP (eGFP Synthetic, Aequorea victoria)

Use

Lentiviral and Synthetic Biology

Expression

Mammalian

Available since

June 5, 2025

Availability

Academic Institutions and Nonprofits only

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eGFP-SUN1

Plasmid

#213515

Purpose

Expresses human eGFP-SUN1 in mammalian cells

Depositor

Karim Mekhail

Publication

Shokrollahi et al Nat Struct Mol Biol. 2024 Sep;31(9):1319

Insert

SUN1 (SUN1 Human)

Expression

Mammalian

Mutation

SUN1 has a silent mutation on its gene (nucleotid…

Promoter

CMV

Available since

May 15, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV_EF1a-fDIO-PdCO-mScarlet-miniWPRE

Plasmid

#198515

Purpose

Expresses optimized PdCO in frame with mScarlet under control of eukaryotic Ef1a promoter after Flp-dependent recombination.

Depositor

Ofer Yizhar

Publication

Wietek et al Nat Methods. 2024 May 29. doi: 10.1038/s

Insert

PdCO

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Promoter

EF-1a

Available since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti6.3 GCN1 R2312A-3xFlag_IRES-iRFP

Plasmid

#198389

Purpose

GCN1 R2312A expresson

Depositor

Jack Taunton

Publication

Oltion et al Cell. 2023 Jan 19;186(2):346-362.e17. do

Insert

GCN1 (GCN1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Mutation

R2312A mutation in RWDBD (RWD binding domain)

Promoter

CMV

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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pEGFP-mKOR

Plasmid

#179285

Purpose

Express mouse kappa opioid receptor (GFP tagged) in mammalian cells

Depositor

Christian Gruber

Publication

Muratspahic et al J Med Chem. 2021 Jul 8;64(13):9042-9055.

Insert

kappa opioid receptor (Oprk1 Mouse)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available since

Feb. 7, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac Dual α1B/β2B Tubulin

Plasmid

#170553

Purpose

Encodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.

Depositor

Tarun Kapoor

Publication

Ti et al Dev Cell. 2018 Oct 22;47(2):175-190.e5.

Inserts

TUBA1B (TUBA1B Human)
TUBB2B (TUBB2B Human)

Tags

AP linker, GS linker (GGSGG), His10 tag, L21 enha…

Expression

Insect

Available since

June 15, 2021

Availability

Academic Institutions and Nonprofits only

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pIRES-Ap1g1-FKBP

Plasmid

#46945

Purpose

Co-expresses siRNA-resistant, FKBP-tagged AP-1 gamma subunit and G418 resistance gene in mammalian cells

Depositor

Margaret Robinson

Publication

Robinson et al Dev Cell. 2010 Feb 16;18(2):324-31. doi:

Insert

Ap1g1-FKBP

Tags

Mainly mouse Ap1g1 cDNA, with a fragment of brain…

Expression

Mammalian

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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HOXB7 (human) pCMV

Plasmid

#8539

Depositor

Corey Largman

Publication

Shen et al Proc Natl Acad Sci U S A 1989 Nov;86(21)

Insert

HOXB7 (HOXB7 Human)

Tags

FLAG

Expression

Mammalian

Mutation

starts at AA#1 preceeded by FLAG and Ser-Arg-Ile…

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
pDONR221_SLC51A

Plasmid

#132240

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Publication

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC51A (SLC51A Human)

Expression

Mammalian

Available since

Nov. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article

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pFGL822

Plasmid

#58225

Purpose

For fungal transformation via ATMT. Transformants to be selected by bialaphos resistance.

Depositor

Naweed Naqvi

Publication

Sweigard et al Fungal Genetics Reports, 1997. Vol. 44,

Insert

phosphinothricin acetyl transferase

Expression

Bacterial

Promoter

Aspergillus nidulans trpC promoter

Available since

June 11, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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pEMG-RIS

Plasmid

#225132

Purpose

For genome editing in P. putida KT2440, rhamnose is used for the induction of I-sceI gene. Km.

Depositor

Lars Blank

Publication

Meng et al Microb Biotechnol. 2024 Jul;17(7):e14531

Type

Empty backbone

Expression

Bacterial

Available since

Oct. 10, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pKMV-VioD

Plasmid

#65350

Purpose

systhesis of vioD gene in the violacein pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioD

Use

Synthetic Biology

Available since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pKMV-VioC

Plasmid

#65349

Purpose

systhesis of vioC gene in the violacein pathway

Depositor

Junbiao Dai

Publication

Guo et al Nucleic Acids Res. 2015 May 8. pii: gkv4

Insert

vioC

Use

Synthetic Biology

Available since

Sept. 17, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pICH47802

Plasmid

#48007

Depositor

Sylvestre Marillonnet

Publication

Weber et al PLoS One. 2011 Feb 18;6(2):e16765. doi:

Type

Empty backbone

Use

Unspecified

Available since

Dec. 3, 2013

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

3 Articles

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EF1α-TetOn3G_2x cHS4_pTRE3G-loxP-EGFP-lox2272 [AAVS1]

Plasmid

#227246

Purpose

All-in-one ROSE LP donor construct for TetOn3G-inducible cell line development in HEK293 cell

Depositor

Jae Seong Lee

Publication

Shin et al Metab Eng. 2024 Sep 5;86:12-28. doi: 10.

Inserts

Tet-On 3G transactivator protein
Enhanced green fluorescent protein

Use

Cre/Lox and Synthetic Biology

Expression

Mammalian

Promoter

EF1α and pTRE3G

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pMIR-CDH1 mutUTR

Plasmid

#25039

Depositor

Bob Weinberg

Publication

Ma et al Nat Cell Biol. 2010 Mar . 12(3):247-56.

Insert

CDH1 mutant 3'UTR (CDH1 Human)

Use

Luciferase

Expression

Mammalian

Mutation

substitution of 6nt within the miR-9 binding site…

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article
pLX304_zeo_eGFP

Plasmid

#160095

Purpose

Expresses eGFP in mammalian cells

Depositor

Rizwan Haq

Publication

Gstalder et al Cancer Discov. 2020 May 5. pii: 2159-829

Insert

eGFP

Use

Lentiviral

Expression

Mammalian

Available since

Oct. 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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2XDR4

Plasmid

#32615

Depositor

Connie Cepko

Publication

Billings et al PLoS One. 2010 . 5(10):e13739.

Insert

2XDR4

Expression

Mammalian

Available since

July 30, 2013

Availability

Academic Institutions and Nonprofits only

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pAG35

Plasmid

#35123

Depositor

John McCusker

Publication

Goldstein et al Yeast. 1999 Oct;15(14):1541-53.

Insert

natMX3

Use

Yeast replicating

Available since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

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MADR pDonor-H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

Plasmid

#131461

Purpose

MADR donor plasmid for FlpO+Cre-mediated insertion of WT H3F3A and oncogenic PDGFRA D842V and TRP53

Depositor

Joshua Breunig

Publication

Kim et al Cell. 2019 Sep 19;179(1):251-267.e24. do

Insert

H3F3A-WT-EGFP pTV1 Pdgfra D842V COTv1 Trp53-V5 WPRE

Use

Cre/Lox and Synthetic Biology; Mosaic analysis fo…

Tags

Trp53-V5, H3F3A-EGFP-AU1

Expression

Mammalian

Mutation

Pdgfra D842V

Promoter

none (4x polyA to mitigate episomal expression)

Available since

July 13, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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