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We narrowed to 8,624 results for: FIE

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Showing: 7801 - 7840 of 8624 results

  3. pCRISPR/eCas9-GPIBY55

    Plasmid
    #213709
    Purpose
    To express a single-guide RNA under a U6 promoter, accompanied by the expression of eCas9 and the affinity sorting tag TST-EGFP-GPIBY55 under separate promoters.
    Depositor
    Qilai Huang
    Article
    Yang et al Front Bioeng Biotechnol. 2022 Jun 16;10:
    Insert
    EGFP
    Use
    CRISPR
    Tags
    3XFLAG and Twin-strep-tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Feb. 16, 2024
    Availability
    Academic Institutions and Nonprofits only
    213709-plasmid-map-sequence-id-422949

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  4. pJL1-sfGFP-MOORmut

    Plasmid
    #198213
    Purpose
    pJL1 plasmid encoding superfolder GFP modified at the C-terminus with 32 amino acids containing a non-permissible minimum optimal O-linked recognition site (MOORmut) followed by a 6x-His tag
    Depositor
    Michael Jewett
    Article
    Hershewe et al Nat Commun. 2021 Apr 22;12(1):2363. doi:
    Insert
    sfGFP_MOORmut
    Tags
    6x His Tag and mutated (non-permissible) MOORmut …
    Expression
    Bacterial
    Promoter
    T7
    Available Since
    May 23, 2023
    Availability
    Academic Institutions and Nonprofits only
    198213-plasmid-map-sequence-id-393674

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  6. kiCAP-AAV-PAL1D

    Plasmid
    #196686
    Purpose
    Rep/Cap plasmid for the production of PAL1D, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    PTQGTFR insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196686-plasmid-map-sequence-id-388011

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  7. kiCAP-AAV-M.Fas.1

    Plasmid
    #196687
    Purpose
    Rep/Cap plasmid for the production of M.Fas.1, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    TDALTTK insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196687-plasmid-map-sequence-id-388008

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  8. kiCAP-AAV-PAL1B

    Plasmid
    #196684
    Purpose
    Rep/Cap plasmid for the production of PAL1B, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    PSQGTLR insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196684-plasmid-map-sequence-id-389747

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  9. kiCAP-AAV-PAL1A

    Plasmid
    #196683
    Purpose
    Rep/Cap plasmid for the production of PAL1A, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    PTQGTVR insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196683-plasmid-map-sequence-id-389740

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  10. kiCAP-AAV-M.Fas.4

    Plasmid
    #196690
    Purpose
    Rep/Cap plasmid for the production of M.Fas.4, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    VVSDYTV insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196690-plasmid-map-sequence-id-389719

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  11. kiCAP-AAV-M.Fas.3

    Plasmid
    #196689
    Purpose
    Rep/Cap plasmid for the production of M.Fas.3, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    RVDPSGL insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    April 7, 2023
    Availability
    Academic Institutions and Nonprofits only
    196689-plasmid-map-sequence-id-389717

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  13. kiCAP-AAV-M.Fas.2

    Plasmid
    #196688
    Purpose
    Rep/Cap plasmid for the production of M.Fas.2, an AAV capsid with CNS tropism in macaques.
    Depositor
    Pardis Sabeti
    Article
    Stanton et al Med (N Y). 2022 Nov 15:S2666-6340(22)004
    Insert
    AAV9 VP1 modified with 7mer insertion between amino acids 588 and 589
    Use
    AAV
    Mutation
    STIPTMK insert between amino acids 588 and 589 of…
    Promoter
    p41
    Available Since
    March 8, 2023
    Availability
    Academic Institutions and Nonprofits only
    196688-plasmid-map-sequence-id-386840

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  15. pEPQDKN0760

    Plasmid
    #185629
    Purpose
    Modified tobacco rattle virus RNA2 vector expressing an sgRNA fused to trucated flowering locus T (BsaI-domesticated) which targets phytoene desaturase (PDS) of Nicotiana benthamiana
    Depositor
    Nicola Patron
    Article
    Dudley et al Commun Biol. 2022 Sep 10;5(1):949. doi:
    Insert
    sgRNA
    Use
    CRISPR and Synthetic Biology
    Available Since
    Aug. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    185629-plasmid-map-sequence-id-368559

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  19. pRSET-TorA-6xHis-M13-pHCountdown-Calmodulin

    Plasmid
    #182305
    Purpose
    E. coli plasmid encoding a fusion between pH-Countdown and the calcium-binding domains of GECO 1.2 with a hexahistidine tag, targeted to the periplasm via the TorA presequence.
    Depositor
    Andrew York
    Article
    Seidel et al (2021), doi: 10.5281/zenodo.5810930
    Insert
    pH-Countdown insertion into GECO 1.2 calcium sensing domains
    Tags
    A modified TorA periplasmic targeting sequence pl…
    Expression
    Bacterial
    Mutation
    Spontaneous mutation of calmodulin's penulti…
    Promoter
    t7
    Available Since
    April 28, 2022
    Availability
    Academic Institutions and Nonprofits only
    182305-plasmid-map-sequence-id-360536

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  23. EGFP-acVHH (Y34W)-PB

    Plasmid
    #171072
    Purpose
    A COSMO mutant (Y34W) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y34W
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171072-plasmid-map-sequence-id-338143

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  24. EGFP-acVHH (Y34F)-PB

    Plasmid
    #171071
    Purpose
    A COSMO mutant (Y34F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y34F
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    171071-plasmid-map-sequence-id-338141

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  25. EGFP–acVHH (Y104F)–PB

    Plasmid
    #169683
    Purpose
    A COSMO mutant (Y104F) responds to caffeine to induce dimerization of a phospholipid-binding polybasic domain with subsequent plasma membrane translocation.
    Depositor
    Yubin Zhou
    Article
    Wang et al Adv Sci (Weinh). 2020 Dec 13;8(3):200214
    Insert
    anti-caffeine nanobody variant
    Tags
    modified STIM1–PB tag
    Expression
    Mammalian
    Mutation
    Y104F
    Promoter
    CMV
    Available Since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    169683-plasmid-map-sequence-id-338137

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Showing: 7801 - 7840 of 8624 results