164,577 results

1. pCEFLmGFP-Gli2

   Plasmid

   #37672

   Depositor

   Philip Beachy

   Article

   Kim et al Proc Natl Acad Sci U S A. 2009 Dec 22;10

   Insert

   Gli2 (Gli2 Mouse)
   

   Use

   Tags

   mGFP

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   July 10, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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2. Bxb1-GA_AIO-TetOn_mScarlet_Divergent

   Plasmid

   #229791

   Purpose

   Bxb1-GA donor plasmid with divergent syntax for all-in-one doxycycline-inducible mScarlet expression

   Depositor

   Richard Davis

   Article

   Blanch-Asensio et al bioRxiv [Preprint]. 2024 Oct 17:2024.10.

   Insert

   TRE-mScarlet; rtTA-T2A-mTagBFP2
   

   Use

   Synthetic Biology

   Tags

   NLS

   Expression

   Mammalian

   Mutation

   Promoter

   TRE and CAG

   Available Since

   Feb. 19, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLenti_Ef1a:PE2-P2A-hMLH1dn

   Plasmid

   #184444

   Purpose

   Single EF1a-driven PE2-P2A-hMLH1dn (Addgene #174828) and PaqCI restriction sites for pegRNA cloning. Lentiviral backbone and AmpR.

   Depositor

   Bobby Koeleman

   Article

   Dirkx et al BMC Biol. 2023 Jul 13;21(1):156. doi: 10

   Type

   Empty backbone

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6 and Ef1A

   Available Since

   Oct. 18, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pSLQ10704

   Plasmid

   #183957

   Purpose

   U6-SKSKSO(crRNA array)-EF1a-PuroR-T2A-BFP

   Depositor

   Stanley Qi

   Article

   Guo et al Nat Cell Biol. 2022 Apr;24(4):590-600. d

   Insert

   SKSKSO array
   

   Use

   Lentiviral

   Tags

   EF1a-Puro-T2A-TagBFP

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available Since

   Aug. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAct-dCBEevoCDA1

   Plasmid

   #195515

   Purpose

   Ubiquitous expression of dCBEevoCDA1 in Drosophila

   Depositor

   Michael Boutros

   Article

   Doll et al Sci Adv. 2023 Sep;9(35):eadj1568. doi: 1

   Insert

   dCBE-evoCDA1
   

   Use

   CRISPR

   Tags

   Expression

   Insect

   Mutation

   Promoter

   act5C

   Available Since

   Sept. 12, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. 4xHRE-MinTK-CRE-ODD

   Plasmid

   #141147

   Purpose

   lentiviral expression vector to generate a hypoxia fate-mapping system

   Depositor

   Daniele Gilkes

   Article

   Godet et al Nat Commun. 2019 Oct 24;10(1):4862. doi:

   Insert

   4xHRE-MinTK-CRE-ODD
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   contains an altered Cre gene modified by the addi…

   Promoter

   Available Since

   Sept. 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pmGFP-ADAR1-p150

   Plasmid

   #117927

   Purpose

   ADAR1-p150 expression plasmid.

   Depositor

   Kumiko Ui-Tei

   Article

   Galipon et al Genes (Basel). 2017 Feb 11;8(2). pii: ge

   Insert

   ADAR1-p150 (ADAR Human)
   

   Use

   Tags

   myc-GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-RSV-SpCas9

   Plasmid

   #85450

   Purpose

   Express SpCas9 in mammalian cells

   Depositor

   Hetian Lei

   Article

   Duan et al J Biol Chem. 2016 May 31. pii: jbc.M116.

   Insert

   pRSV
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   pRSV

   Available Since

   Jan. 26, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHAGE-EGFR-E746_T751delinsA

   Plasmid

   #116246

   Purpose

   Lentiviral expression of EGFR E746_T751delinsA

   Depositor

   Kenneth Scott, Gordon Mills

   Article

   Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

   Insert

   EGFR (EGFR Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   E746_T751delinsA

   Promoter

   Available Since

   March 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAG416GAL-ccdB

    Plasmid

    #14147

    Depositor

    Susan Lindquist

    Article

    Alberti et al Yeast. 2007 Oct . 24(10):913-9.

    Has Service

    Cloning Grade DNA

    Type

    Empty backbone

    Use

    Gateway destination

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    March 16, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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11. ∆DUSP6

    Plasmid

    #27977

    Depositor

    Igor Astsaturov

    Article

    Bagnyukova et al Br J Cancer. 2013 Aug 20;109(4):1063-71.

    Insert

    DUSP6 (DUSP6 Human)
    

    Use

    Lentiviral

    Tags

    HA and MYC

    Expression

    Mammalian

    Mutation

    C293S: mutation in the phosphatase domain

    Promoter

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

12. M31: pHR-SFFVp- TDP-43C::mCherry::SspB

    Plasmid

    #122669

    Purpose

    C terminal domain of TDP43 (218-414) tagged with SspB and mCherry.

    Depositor

    Clifford Brangwynne

    Article

    Bracha et al Cell. 2018 Nov 29;175(6):1467-1480.e13.

    Insert

    TDP43 (TARDBP Human)
    

    Use

    Lentiviral

    Tags

    mCherry-SspB

    Expression

    Mammalian

    Mutation

    Contains only amino acids 218-414

    Promoter

    SFFV

    Available Since

    April 1, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDmAct5C::Cas9-2A-Neo

    Plasmid

    #176679

    Purpose

    Expression of Drosophila codon optimized spCas9 under Drosophila Act5C promoter

    Depositor

    Norbert Perrimon

    Article

    Viswanatha et al Nat Commun. 2021 Nov 24;12(1):6825. doi:

    Insert

    spCas9; NeoR (aminoglycoside phosphotransferase from Tn5)
    

    Use

    Crispr

    Tags

    Expression

    Insect

    Mutation

    Drosophila codon optimized

    Promoter

    D. melanogaster Act5C

    Available Since

    Nov. 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pU6-sgRNA EF1Alpha-puro-T2A-BFP

    Plasmid

    #60955

    Purpose

    Parental vector for the CRISPRi/a libraries. Expresses an sgRNA from the U6 promoter and a puromycin resistance cassette and BFP from the EF1Alpha promoter

    Depositor

    Jonathan Weissman

    Article

    Gilbert et al Cell. 2014 Oct 23;159(3):647-61. doi: 10

    Inserts

    sgGFP-NT2
    puro-T2A-BFP
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 9, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV PIG Fli1

    Plasmid

    #66983

    Purpose

    Retroviral expression plasmid for mouse Fli1 expression; containing bicistronic IRES-GFP

    Depositor

    Christopher Vakoc

    Article

    Roe et al Mol Cell. 2015 May 12. pii: S1097-2765(1

    Insert

    Fli1 (Fli1 Mouse)
    

    Use

    Retroviral

    Tags

    Expression

    Mutation

    Promoter

    LTR

    Available Since

    July 15, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pcDNA-puro-kozak-scFv-GCN4-HAtag-msfGFP(V206K)-GBI

    Plasmid

    #205162

    Purpose

    express scFv-GCN4-HAtag-msfGFP(V206K)-GBI

    Depositor

    Weirui Ma

    Article

    Hu et al Elife. 2023 Mar 3;12:e82178. doi: 10.755

    Insert

    scFv-GCN4-HAtag-msfGFP(V206K)-GBI
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Oct. 18, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. p(HA)HIF1alpha (P402A,P564A)

    Plasmid

    #52636

    Purpose

    mammalian expression of HA tagged HIF1alpha (full length) with P402A,P564A mutation

    Depositor

    Eric Huang

    Article

    Kageyama et al FASEB J. 2004 Jun;18(9):1028-30. Epub 20

    Insert

    HIF1alpha P402A, P564A (HIF1A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    P402A, P564A

    Promoter

    CMV

    Available Since

    April 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCI-His-hUbi

    Plasmid

    #31815

    Depositor

    Astar Winoto

    Article

    Young et al J Biol Chem. 2011 Feb 25;286(8):6521-31.

    Insert

    Ubiquitin (UBB Human)
    

    Use

    Tags

    HIS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available Since

    Sept. 6, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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19. VCP(wt)-EGFP

    Plasmid

    #23971

    Depositor

    Nico Dantuma

    Article

    Tresse et al Autophagy. 2010 Feb 22. 6(2):.

    Insert

    Valosin containing protein (VCP Human)
    

    Use

    Tags

    6xHis, EGFP, and HA

    Expression

    Mammalian

    Mutation

    none

    Promoter

    Available Since

    Feb. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-hSyn-EGFP

    Plasmid

    #50465

    Purpose

    EGFP under the control of human synapsin promoter

    Depositor

    Bryan Roth

    Has Service

    AAV PHP.eB, AAV Retrograde, AAV Retrograde trial size, AAV1, AAV1 trial size, AAV11, AAV11 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, and AAV9 trial size

    Insert

    EGFP
    

    Use

    AAV

    Tags

    N/A

    Expression

    Mutation

    Promoter

    human Synapsin 1

    Available Since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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