We narrowed to 19,278 results for: CAL

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EcMBP165-cpGFP.PPYF.T203V.pRSET

Plasmid

#33373

Purpose

a single-wavelength maltose sensor constructed from E. coli MalE and cpGFP

Depositor

Loren Looger

Article

Marvin et al Proteins. 2011 Nov;79(11):3025-36. doi:

Insert

Improved MBP based Maltose Biosensor

Tags

6xHIS

Expression

Bacterial

Mutation

cpGFP146 variant from GCaMP2 inserted between AA1…

Promoter

T7

Available Since

Dec. 21, 2011

Availability

Academic Institutions and Nonprofits only

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pCS2+ MT Tcf3 (Xenopus)

Plasmid

#13439

Depositor

Marc Kirschner

Article

Lee et al J Cell Biol. 2001 Sep 3. 154(5):983-93.

Insert

Tcf3 (tcf7l1.S Frog)

Tags

Myc Tag

Expression

Mammalian

Available Since

Nov. 1, 2006

Availability

Academic Institutions and Nonprofits only
pLViP-LifeAct-iRFP670

Plasmid

#229699

Purpose

Lentiviral expression of LifeAct-iRFP670 in mammalian cells

Depositor

Alpha Yap

Article

Noordstra et al Dev Cell. 2023 Sep 25;58(18):1748-1763.e

Insert

Synthetic construct LifeAct-mTurquoise gene

Use

Lentiviral

Tags

iRFP670

Expression

Mammalian

Promoter

CMV promoter

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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2MLS-HyperNova/pcDNA3

Plasmid

#221457

Purpose

Expressing HyperNova in mitochondria

Depositor

Kiwamu Takemoto

Article

Shidara et al Commun Biol. 2024 Aug 6;7(1):945. doi: 1

Insert

HyperNova fused with a tandem repeat of the mitochondria localization signal from cytochrome c oxidase subunit VIII

Expression

Mammalian

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pCDNA-FRT-FAK20

Plasmid

#59120

Purpose

FAK fret sensor with 20 nm helix

Depositor

Sivaraj Sivaramakrishnan

Article

Ritt et al J Biol Chem. 2013 Mar 29;288(13):8875-86

Insert

Focal Adhesion Kinase

Tags

FLAG, mCerulean, and mCitrine

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-Cep152

Plasmid

#136825

Purpose

Mammalian expression of the centrosomal protein Cep152 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Article

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Insert

SNAP-Cep152 (CEP152 Human, Synthetic)

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available Since

March 26, 2020

Availability

Academic Institutions and Nonprofits only

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pHR-BRD4ΔN-miRFP670-Cry2WT

Plasmid

#122439

Purpose

Expresses disordered protein BRD4(462-1362) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

Insert

BRD4 (BRD4 Human)

Use

Lentiviral

Tags

Cry2WT and miRFP670

Expression

Mammalian

Mutation

Deleted amino acids 1-461

Promoter

SFFV

Available Since

April 10, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-S100A11

Plasmid

#107201

Purpose

Expresses GFP-human S100A11 fusion protein for live cell imaging

Depositor

Volker Gerke, Ursula Rescher

Insert

S100A11 (S100A11 Human)

Tags

Green Fluorescent Protein

Expression

Mammalian

Promoter

CMV

Available Since

June 13, 2018

Availability

Academic Institutions and Nonprofits only

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pCMV-Y-RAN-GFP26

Plasmid

#106423

Purpose

Y-RAN-GFP26 (RANbody GFP26::Chicken IgY)

Depositor

Joshua Sanes

Article

Yamagata et al Proc Natl Acad Sci U S A. 2018 Feb 13. p

Insert

Y-RAN-GFP26

Tags

HA and His

Expression

Mammalian

Available Since

Feb. 20, 2018

Availability

Academic Institutions and Nonprofits only

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3xFLAG-dCas9/MSCV-EGFP

Plasmid

#82613

Purpose

Expresses 3xFLAG-dCas9 in mammalian cells for enChIP analysis to purify specific genomic regions of interest.

Depositor

Hodaka Fujii

Article

Fujita et al Genes Cells. 2018 Feb 26. doi: 10.1111/g

Insert

3xFLAG-dCas9

Use

Retroviral

Tags

3xFLAG tag and NLS (nuclear localization signal)

Mutation

human codon-optimized, D10A + H840A

Promoter

LTR

Available Since

Sept. 16, 2016

Availability

Academic Institutions and Nonprofits only

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AIP(WT)-mChF-giantin

Plasmid

#61523

Purpose

Expression of human golbin B1 and FK506 binding protein 1A (FKBP1A) tagged with mCherry and AIP

Depositor

Takanari Inoue

Article

Miyamoto et al Cell Rep. 2015 Apr 28;11(4):657-70. doi:

Inserts

FK506 binding protein 1A (FKBP1A Human)
golgin B1 (GOLGB1 Human)

Tags

AIP and mCherry

Expression

Mammalian

Promoter

CMV

Available Since

April 17, 2015

Availability

Academic Institutions and Nonprofits only
pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)

Plasmid

#48296

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

His6 and MBP

Expression

Insect

Available Since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA-mito-ZifCY1

Plasmid

#36317

Depositor

Amy Palmer

Article

Dittmer et al J Biol Chem. 2009 Jun 12;284(24):16289-9

Insert

mito-ZifCY1 genetically encoded Zn(II) sensor

Tags

see comment

Expression

Mammalian

Promoter

CMV

Available Since

July 31, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat FL

Plasmid

#32449

Depositor

Anthony Means

Article

Anderson et al Cell Metab. 2008 May;7(5):377-88.

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S3L, G96R, and P108L

Promoter

SV40

Available Since

Feb. 1, 2012

Availability

Academic Institutions and Nonprofits only
pSF11-wNIR-GECO2-T2A-HO1

Plasmid

#159606

Purpose

Codon-optimized near-infrared fluorescent calcium indicator NIR-GECO2 with codon-optimized heme-oxygenase in C.elegans expression plasmid

Depositor

Robert Campbell

Article

Qian et al PLoS Biol. 2020 Nov 24;18(11):e3000965.

Insert

wNIR-GECO2-T2A-HO1

Expression

Worm

Promoter

tag-168

Available Since

Sept. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 S472E

Plasmid

#73053

Purpose

Human AKT3 S472E

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 S472E (AKT3 Human)

Expression

Mammalian

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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pCIG AKT3 K177M

Plasmid

#73051

Purpose

Human AKT3 K177M

Depositor

Joseph Gleeson

Article

Baek et al Nat Med. 2015 Dec;21(12):1445-54. doi: 1

Insert

AKT3 K177M (AKT3 Human)

Expression

Mammalian

Mutation

K177M

Available Since

March 11, 2016

Availability

Academic Institutions and Nonprofits only

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spa-GCaMP6s

Plasmid

#67556

Purpose

photoactivatable fluorescent calcium reporter

Depositor

Ehud Isacoff

Article

Berlin et al Nat Methods. 2015 Jul 13. doi: 10.1038/n

Insert

spa-GCaMP6s

Tags

HIS-tag

Expression

Mammalian

Mutation

K65T, I83V, G87S, Y92D, V115H, K118V, S187R, Y196…

Available Since

July 28, 2015

Availability

Academic Institutions and Nonprofits only
pH6HTC_CCT2-Halo

Plasmid

#175337

Purpose

Production of HaloTag fusion protein

Depositor

Claudia Kutter

Article

Atanasoai et al bioRxiv 2021.11.08.467743

Insert

CCT2 (CCT2 Human)

Use

Cell free expression

Tags

HaloTag-6xHis

Expression

Bacterial

Available Since

Oct. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBW2663_pCAG-PV1-PYL-L1-iCre-271C-NLS-BGHpA

Plasmid

#108746

Purpose

Expresses aa271 to C-terminus of Cre recombinase fused to PYL CID

Depositor

Wilson Wong

Article

Weinberg et al Nat Commun. 2019 Oct 24;10(1):4845. doi:

Insert

Cre recombinase fragment aa271 to C-terminus

Use

Cre/Lox and Synthetic Biology

Tags

NLS and PYL

Expression

Mammalian

Promoter

pCAG

Available Since

Aug. 28, 2019

Availability

Academic Institutions and Nonprofits only

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