We narrowed to 27,700 results for: CAL;
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Plasmid#100855PurposeAAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the CAG promoterDepositorInsertjRGECO1b
UseAAVExpressionMammalianPromoterCAGAvailable SinceFeb. 28, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDule2-3-nitroTyrosine (5B)
Plasmid#85499PurposePlasmid for incorporating the non-canonical amino acid 3-nitroTyrosine with the Mj 3NY (5B) synthetase and cognate amber suppressing tRNA in Ecoli.DepositorInsert3-nitroTyrosine tRNA synthetase and cognate amber suppressing tRNA derived from M. jannaschii Tyrosine synthetase/tRNA system
ExpressionBacterialMutationY32H H70C D158S I159A L162RPromoterlpp (constitutive)Available SinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTol2-CreLite
Plasmid#131783PurposeTol2 destination vector carrying Ubi:CreLite with mTagBFP2 as a visual marker.DepositorInsertPhyBCreC, PIF6CreN, and mTagBFP2
UseCre/Lox and Synthetic Biology; Zebrafish tol2 tra…TagsmTagBFP2ExpressionMammalianPromoterUbiAvailable SinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
LITE2.0 EF1a_TALE(Neurog2)-GS-CIB1(NLS*,∆318-334)_WPRE_hGHpA
Plasmid#47458PurposeLITE2.0 TALE-CIB1. Changes from LITE1.0: glycine-serine linker, mutated endogenous NLS, aa318-334 deletion. This particular TALE targeted to Neurog2. EF1-alpha promoter for ubiquitous mammalian exp.DepositorInsertTALE (Neurog2)-GS-CIB1(NLS*,∆318-334)
TagsHAExpressionMammalianPromoterEF1-alphaAvailable SinceOct. 22, 2013AvailabilityAcademic Institutions and Nonprofits only -
Cav2.2 BBS pcDNA3
Plasmid#206057Purposeexpression of rabbit Cav2.2 calcium channel with an exofacial double Bungarotoxin Binding Site motif in domain IIDepositorAvailable SinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
SspB-HaloTag-DHPH-TIAM1
Plasmid#176114PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
ExpressionMammalianAvailable SinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2-IRES-dOrai-IRES-GFP
Plasmid#72893PurposeMammalian expression of dmBACCS2, a Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorInsertdmBACCS2-IRES-dOrai-IRES-GFP
TagsIRES-GFPExpressionMammalianPromoterCMVAvailable SinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJRoC30_Th3.14
Plasmid#188068PurposeFuncLib-designed high-redox potential laccase from Trametes hirsuta (Th3.14)DepositorInsertFuncLib-designed high-redox potential laccase from Trametes hirsuta
TagsS. cerevisiae evolved α-factor prepro-leader sign…ExpressionYeastMutation20 PROSS + 4 FuncLib mutations, described in publ…Available SinceOct. 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
SspB-mCherry-DHPH-TIAM1
Plasmid#176104PurposeHeterodimerization with iLID, Rac1 activationDepositorInsertTIAM1 (TIAM1 Human)
ExpressionMammalianAvailable SinceOct. 27, 2021AvailabilityAcademic Institutions and Nonprofits only -
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pDisplay-GACh1.0
Plasmid#106072Purposeexpress the genetically-encoded fluorescent ACh indicator GACh1.0 in mammalian cellsDepositorInsertGPCR activation based ACh sensor (GACh) 1.0
ExpressionMammalianPromoterCMVAvailable SinceApril 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAAV.Syn.Flex.NES-jRGECO1b.WPRE.SV40
Plasmid#100856PurposeAAV expression of Cre recombinase-activated jRGECO1b, a red fluorescent calcium sensor protein, from the Synapsin promoterDepositorInsertjRGECO1b
UseAAVExpressionMammalianPromoterSynapsinAvailable SinceJuly 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
dmBACCS2NS-IRES-dOrai-IRES-GFP
Plasmid#72895PurposeMammalian expression of dmBACCS2NS, a modified Drosophila melanogaster blue light-activated Ca2+ channel switch, along with dOrai and GFPDepositorInsertdmBACCS2NS-IRES-dOrai-IRES-GFP
TagsIRES-GFPExpressionMammalianPromoterCMVAvailable SinceJan. 27, 2016AvailabilityAcademic Institutions and Nonprofits only -
mTiam1-mcherry-sspB in pcDNA3.1
Plasmid#85221PurposeRole of Rac selective GEF domain from Tiam1 in immune cell migrationDepositorAvailable SinceJan. 5, 2017AvailabilityAcademic Institutions and Nonprofits only -
3_T7-mScarlet3-KRas6R
Plasmid#225930PurposeIn vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras6R membrane localisation signalDepositorInsertmScarlet3-KRas6R
UseIn vitro transcriptionPromoterT7Available SinceNov. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pICE-HA-CtIP-siR-WT
Plasmid#82030PurposePlasmid for constitutive or doxycycline-inducible expression of human CtIP resistant to a siRNA. Confers resistance to puromycin. Use T-REx cells for doxycycline-inducible expression.DepositorInsertCtIP (RBBP8 Human)
TagsHAExpressionMammalianMutationMutations making the cDNA resistant to a siRNAPromoterCMV-tetAvailable SinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSUC2::GNSI (NrsR)
Plasmid#121537PurposeThis plasmid contains a CYC1pr driven GNSI reporter and flanking homology to SUC2. Digestion with NotI, SacI, and EcoRV, allows integration at the SUC2 locus.DepositorInsertsCYC1 Promoter (CYC1 Budding Yeast)
Nyv1 Cytosolic Domain (aa 6-230)
Snc1 Transmembrane Domain (TMD)
SUC2 CDS
SUC2 terminator
NrsR
TagsmGFP5ExpressionYeastMutationM109I (Naturally occurring SNP) and The first 21 …Available SinceFeb. 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPKm-105
Plasmid#104853PurposepcDNA3 - pCMV - PhyB NT - GBD, expressing Phytochrome B (PhyB) with Gal Binding Domain (GBD), under CMV promoterDepositorInsertPhyB NT GBD
ExpressionMammalianPromoterCMVAvailable SinceMarch 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pHR-TAF15N-miRFP670-Cry2WT
Plasmid#122440PurposeExpresses disordered protein TAF15(1-208) fused with fluorescent protein miRFP670 and optogenetic protein Cry2WTDepositorInsertTAF15 (TAF15 Human)
UseLentiviralTagsCry2WT and miRFP670ExpressionMammalianMutationContains only amino acids 1-208PromoterSFFVAvailable SinceMarch 11, 2019AvailabilityAcademic Institutions and Nonprofits only