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pWZL Neo Myr Flag PIP5K2A

Plasmid

#20530

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

PIP5K2A (PIP4K2A Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pWZL Neo Myr Flag MAP3K14

Plasmid

#20640

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

MAP3K14 (MAP3K14 Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pmCherry-T2A-moxMaple3

Plasmid

#120875

Purpose

Mammalian expression of mCherry and moxMaple3

Depositor

Vladislav Verkhusha

Article

Kaberniuk et al Sci Rep. 2018 Oct 3;8(1):14738. doi: 10.

Inserts

mCherry
moxMaple3

Expression

Mammalian

Mutation

from mMaple3, moxMaple3 was engineered by replaci…

Available Since

Jan. 28, 2019

Availability

Academic Institutions and Nonprofits only

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TC9pAB

Plasmid

#71310

Purpose

used as a template for in vitro transcription of Cas9 mRNA after linearizing with BbsI

Depositor

Karl Degenhardt

Insert

Cas9

Use

In vitro transcription

Tags

NLS

Promoter

T7

Available Since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pHA1

Plasmid

#85709

Purpose

This plasmid provides a BAC vector backbone, which can be used for cloning of BAC inserts, for instance, herpesvirus genomes

Depositor

Ulrich Koszinowski, Martin Messerle

Article

Adler et al J Virol. 2000 Aug;74(15):6964-74.

Type

Empty backbone

Use

Cre/Lox; Herpesvirus cloning

Expression

Bacterial

Available Since

June 28, 2019

Availability

Academic Institutions and Nonprofits only

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TCF7L2_pLX307

Plasmid

#98373

Purpose

Lentiviral expression of TCF7L2

Depositor

William Hahn, Sefi Rosenbluh

Article

Rosenbluh et al Cell Syst. 2016 Sep 28;3(3):302-316.e4.

Insert

TCF7L2 (TCF7L2 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Isoform X25

Promoter

E1Fa

Available Since

Aug. 24, 2017

Availability

Academic Institutions and Nonprofits only

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pBT312_pH11-U-attP-FRT5-pSV40-Neo-pA-pVasa-PhiC31o-pA-FRT5

Plasmid

#52545

Purpose

Targeting vector for homologous recombination in mouse ES cells; Hipp11 locus

Depositor

Liqun Luo

Article

Tasic et al Proc Natl Acad Sci U S A. 2011 May 10;10

Inserts

PhiC31o attP (full length)
Neo
PhiC31o integrase

Expression

Mammalian

Available Since

Aug. 20, 2014

Availability

Academic Institutions and Nonprofits only

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pVC228 VEGF Site#3

Plasmid

#47507

Purpose

human gRNA expression vector targeting VEGF

Depositor

Keith Joung

Article

Fu et al Nat Biotechnol. 2013 Jun 23. doi: 10.103

Insert

gRNA-VEGF site 3 (VEGFA Human)

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Aug. 29, 2013

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-RRR

Plasmid

#48636

Purpose

Expression of TALEN proteins with FokI-RRR domain

Depositor

David Grunwald

Article

Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

Type

Empty backbone

Use

TALEN

Tags

HA-1 and HA-2

Promoter

CMV

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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EC64420

Plasmid

#209517

Purpose

Cloning in complete guide expression cassettes, with Cas9

Depositor

Wendy Harwood, Cristobal Uauy

Article

Lawrenson et al Plant Methods. 2024 Aug 13;20(1):123. do

Type

Empty backbone

Use

CRISPR

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pCS2TAL3-DDD

Plasmid

#48637

Purpose

Expression of TALEN proteins with FokI-DDD domain

Depositor

David Grunwald

Article

Hu et al PLoS One. 2013 May 7;8(5):e63074. doi: 1

Type

Empty backbone

Use

TALEN

Tags

Flag

Promoter

CMV

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pMM534

Plasmid

#112530

Purpose

pZE2-PLhrtO-luxCDABE - Tandem LuxCDABE expressed constitutively from promoter PLhrtO, ColE1 origin, KanR

Depositor

Timothy Lu

Article

Mimee et al Science. 2018 May 25;360(6391):915-918.

Insert

luxCDABE

Expression

Bacterial

Promoter

PL(HrtO)

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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N370S GBA pcDNA3.1

Plasmid

#188583

Purpose

Ectopic expression N370S GBA

Depositor

Anthony Schapira

Article

Smith et al Hum Mol Genet. 2022 Sep 20:ddac233. doi:

Insert

GBA (GBA1 Human)

Expression

Mammalian

Mutation

N370S

Promoter

CMV

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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eGFP-KIF5B-Rigor

Plasmid

#172204

Purpose

Expresses KIF5B G234A rigor mutant tagged with eGFP in mammalian cells

Depositor

Juan Bonifacino

Article

Guardia et al Cell Rep. 2016 Nov 15;17(8):1950-1961. d

Insert

KIF5B (Kif5b Mouse)

Tags

eGFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pAC92-pmax-dCas9VP96

Plasmid

#48224

Purpose

dCas9VP96 on pmax expression vector

Depositor

Rudolf Jaenisch

Article

Cheng et al Cell Res. 2013 Aug 27. doi: 10.1038/cr.2

Insert

dCas9(D10A;H840A) fusion with VP96 activation domain

Use

CRISPR

Tags

HA Tag and VP96

Expression

Mammalian

Mutation

D10A;H840A

Promoter

CAGGS

Available Since

Sept. 17, 2013

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT PUR mGSK3AWT

Plasmid

#35159

Depositor

Jim Woodgett

Article

Doble et al Dev Cell. 2007 Jun;12(6):957-71.

Insert

GSK-3α (Gsk3a Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

May 18, 2012

Availability

Industry, Academic Institutions, and Nonprofits
pWZL Neo Myr Flag CAMK2B

Plasmid

#20439

Depositor

William Hahn, Jean Zhao

Article

Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

Insert

CAMK2B (CAMK2B Human)

Use

Retroviral

Tags

Flag and Myr

Expression

Mammalian

Available Since

Oct. 27, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA3-myc-his-BACH1 P47A

Plasmid

#17644

Depositor

Ronny Drapkin

Article

Cantor et al Proc Natl Acad Sci U S A. 2004 Feb 24. 1

Insert

BACH1 (BRIP1 Human)

Tags

His and Myc

Expression

Mammalian

Mutation

To generate the tumor-associated mutant, the prol…

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only
U6-BbsI-GGsite-mCherry-Puro

Plasmid

#221232

Purpose

Cloning vector for sgRNA with mCherry and Puro

Depositor

Fei Chen

Article

Chen et al Science. 2024 Oct 11;386(6718):eadn5876.

Type

Empty backbone

Expression

Mammalian

Available Since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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pCB24 pDEST crybb1:TagBFP

Plasmid

#195987

Purpose

Transgenesis backbone

Depositor

Markus Affolter, Christian Mosimann

Article

Kemmler et al Development. 2023 Mar 28:dev.201531. doi

Type

Empty backbone

Use

Other

Available Since

Feb. 13, 2023

Availability

Academic Institutions and Nonprofits only

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