We narrowed to 10,320 results for: plasmids 101

1. pDDGFP-Leu2d-GgKDELR2 R47K

   Plasmid

   #123621

   Purpose

   Expresses GgKDELR2 with Arg47Lys mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Arg47Lys

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDDGFP-Leu2d-GgKDELR2 E127A

   Plasmid

   #123622

   Purpose

   Expresses GgKDELR2 with Glu127Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Glu127Ala

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pDDGFP-Leu2d-GgKDELR2 E127Q

   Plasmid

   #123623

   Purpose

   Expresses GgKDELR2 with Glu127Gln mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Glu127Gln

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDDGFP-Leu2d-GgKDELR2 Y158F

   Plasmid

   #123624

   Purpose

   Expresses GgKDELR2 with Tyr158Phe mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Tyr158Phe

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pDDGFP-Leu2d-GgKDELR2 R159A

   Plasmid

   #123625

   Purpose

   Expresses GgKDELR2 with Arg159Ala mutation in S. cerevisiae cells using Galactose induction, -URA and -Leu selection

   Depositor

   Simon Newstead

   Article

   Brauer et al Science. 2019 Mar 8;363(6431):1103-1107.

   Insert

   KDELR2 (KDELR2 Chicken)
   

   Use

   Tags

   TEV-GFP-His

   Expression

   Yeast

   Mutation

   Arg159Ala

   Promoter

   Available Since

   May 20, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV hPGRN

   Plasmid

   #213682

   Purpose

   Expresses human progranulin (PGRN) with an N-terminal twin-Strep-V5 tag

   Depositor

   Thomas Kukar

   Article

   Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

   Insert

   Human Progranulin (hPGRN) (GRN Human)
   

   Use

   AAV

   Tags

   Twin-Strep tag and V5 tag (after signal peptide)

   Expression

   Mutation

   Promoter

   cytomegalovirus enhancer/chicken β-actin promoter

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. hSyn-HA-PGRN-LAMP1TM-IRES-GFP

   Plasmid

   #234877

   Purpose

   Lentiviral plasmid expressing HA-tagged human progranulin that is fused to the transmembrane domain and cytosolic tail of LAMP-1. Enables lysosomal delivery of progranulin without secretion.

   Depositor

   Andrew Arrant

   Article

   Davis et al J Biol Chem. 2021 Sep;297(3):100993. doi

   Insert

   Progranulin (GRN Human)
   

   Use

   Lentiviral

   Tags

   HA tag and LAMP-1 transmembrane domain and cytoso…

   Expression

   Mammalian

   Mutation

   Promoter

   hSYn

   Available Since

   April 24, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTRIP-hPGK-Blast-2A-STING-mNeonGreen

   Plasmid

   #227186

   Purpose

   Lentiviral expression plasmid encoding STING-mNeonGreen under hPGK promoter

   Depositor

   Nir Hacohen

   Article

   Gentili et al Cell Syst. 2024 Dec 18;15(12):1264-1277.

   Insert

   STING1 (STING1 Human)
   

   Use

   Lentiviral

   Tags

   mNeonGreen

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   July 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV hGRN4

   Plasmid

   #213684

   Purpose

   Expresses human granulin-4 with an N-terminal twin-Strep-FLAG tag

   Depositor

   Thomas Kukar

   Article

   Root et al Cell Rep. 2024 Nov 19;43(12):114985. doi

   Insert

   Human granulin-4 (hGRN4) (GRN Human)
   

   Use

   AAV

   Tags

   Twin-Strep tag and FLAG tag (after signal peptide…

   Expression

   Mutation

   Promoter

   cytomegalovirus enhancer/chicken β-actin promoter

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. Lenti-RNF2-ngRNA+41_EF1a-puroR

    Plasmid

    #207358

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a RNF2 nicking guide RNA and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    RNF2 +41 ngRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. 6xHis-MBP-BrCas12b-RFND

    Plasmid

    #195339

    Purpose

    Expresses Engineered BrCas12b in Bacteria

    Depositor

    Piyush Jain

    Article

    Nguyen et al Cell Rep Med. 2023 May 16;4(5):101037. d

    Insert

    BrCas12b
    

    Use

    CRISPR and Synthetic Biology

    Tags

    6xHis and 6xHis, MBP, TEV cleavage site

    Expression

    Bacterial

    Mutation

    R160E, F208W, N524V, D868V

    Promoter

    T7

    Available Since

    May 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. Lenti-N1303K-ngRNA+14_EF1a-puroR

    Plasmid

    #207359

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of N1303K-CFTR via prime editing and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    N1303K-CFTR +14 ngRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. Lenti-L227R-ngRNA+15_EF1a-puroR

    Plasmid

    #207360

    Purpose

    Lentiviral transfer plasmid encoding hU6-driven expression of a ngRNA for correction of L227R-CFTR via prime editing and EF1a-driven puromycin resistance gene.

    Depositor

    Marianne Carlon

    Article

    Bulcaen et al Cell Rep Med. 2024 May 21;5(5):101544. d

    Insert

    L227R-CFTR +15 ngRNA
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    April 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pAAV-EGFP.T2A.NCLX.3'UTR

    Plasmid

    #181872

    Purpose

    Expresses EGFP and murine NCLX (separated by a T2A cleavage site) under control of the synthetic CAG promoter (includes a large portion of the Slc8b1 3'UTR)

    Depositor

    Hilmar Bading

    Article

    Hagenston et al J Biol Chem. 2021 Dec 20:101508. doi: 10

    Inserts

    solute carrier family 8 member B1 (Slc8b1 Mouse)
    EGFP
    3'UTR of solute carrier family 8 member B1
    

    Use

    AAV and Adenoviral

    Tags

    HA, T2A, and myc

    Expression

    Mammalian

    Mutation

    includes a large portion of the Slc8b1 3'UTR…

    Promoter

    synthetic hybrid CAG promoter

    Available Since

    March 7, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. DNA-CARzeta-GFP

    Plasmid

    #89344

    Purpose

    2nd generation lentiviral vector which expresses DNA-CAR receptor fused to GFP

    Depositor

    Ron Vale

    Article

    Taylor et al Cell. 2017 Mar 23;169(1):108-119.e20. do

    Insert

    DNA-CARzeta (CD247, CD86 Human)
    

    Use

    Lentiviral

    Tags

    SNAPf tag and mEGFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. Gal4-TET1-878-911-WT(pL84)

    Plasmid

    #124400

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of WT TET1 SID.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911 wildtype (TET1 Human)
    

    Use

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. psicheck-2-LPPRC

    Plasmid

    #48167

    Purpose

    contains Renilla Luciferase gene preceded by an intact (ATG) upstream open reading frame element

    Depositor

    Vamsi Mootha

    Article

    Calvo et al Proc Natl Acad Sci U S A. 2009 May 5;106

    Insert

    LRPPRC leucine rich pentatricopeptide repeat containing (Lrpprc Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Oct. 16, 2013

    Availability

    Academic Institutions and Nonprofits only

18. Gal4-TET1-878-911-I894A(pL84)

    Plasmid

    #124404

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with I894A mutation.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911(I894A mutation) (TET1 Human)
    

    Use

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    I894A

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. Gal4-TET1-878-911-T898A(pL84)

    Plasmid

    #124403

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with T898A mutation.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911(T898A mutation) (TET1 Human)
    

    Use

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    T898A

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Gal4-TET1-878-911-L900A(pL84)

    Plasmid

    #124402

    Purpose

    Gal4/VP16 luciferase reporter assay. Gal4 DBD fusion of TET1 SID with L900A mutation.

    Depositor

    Shaun Cowley

    Article

    Chandru et al Sci Rep. 2018 Oct 2;8(1):14689. doi: 10.

    Insert

    Gal4-TET1-878-911(L900A mutation) (TET1 Human)
    

    Use

    Tags

    gal4 dna binding domain

    Expression

    Mammalian

    Mutation

    L900A

    Promoter

    Available Since

    May 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge