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Plasmid#232910PurposePlasmid for marker free integration of E. coli appA at the phac1 locus of Cupriavidus necator.DepositorInsertAppA (appA )
UseSynthetic BiologyTagsExpressionMutationPromoterPj5Available sinceMay 14, 2025AvailabilityAcademic Institutions and Nonprofits only -
pYihI-N-term D-N/E-Q
Plasmid#233095PurposeExpression of GST-YihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)DepositorInsertYihI with N-term aspartic acids (D) mutated to asparagine (N) and glutamic acids (E) mutated to glutamine (Q)
UseTagsGSTExpressionBacterialMutationN-term aspartic acids (D) mutated to asparagine (…PromoterAvailable sinceMay 7, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
pYihI-N-term D-A/E-L
Plasmid#233097PurposeExpression of GST-YihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)DepositorInsertYihI with N-term aspartic acids (D) mutated to alanine (A) and glutamic acids (E) mutated to leucine (L)
UseTagsGSTExpressionBacterialMutationN-term aspartic acids (D) mutated to alanine (A) …PromoterAvailable sinceMay 7, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
GFP-mTSP15
Plasmid#236542PurposeFor expression of Tspan15 in mammalian cellsDepositorInsertTSPAN15 (Tspan15 Mouse)
UseTagsGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
GFP-mTSP33
Plasmid#236541PurposeFor expression of Tspan33 in mammalian cellsDepositorInsertTSPAN33 (Tspan33 Mouse)
UseTagsGFPExpressionMammalianMutationWTPromoterAvailable sinceApril 24, 2025AvailabilityAcademic Institutions and Nonprofits only -
pMaSat-miRFP670 pc3944
Plasmid#201695PurposeExpresses of miRFP-tagged MaSat in mammalian cellsDepositorInsertMaSat Synthetic construct zinc finger
UseTagsmiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceApril 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pNDGG002
Plasmid#231308PurposeVector part for BEVA; BEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpRDepositorInsertBEVA2.0 Position 2 Sp antibiotic resistance via aadA, AmpR/SpR
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
plko_TRE3G_Myc-Sox9HMGonlySTOP_PGKpuroR
Plasmid#203963PurposeDox inducible myc tag truncated SOX9 without trans-activating domain (noTA)DepositorInsertSOX9-noTA (Sox9 Mouse)
UseLentiviralTagsmycExpressionMutationthe TA domain is truncatedPromoterTRE3GAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
plko_TRE3G_nlsMyc-Sox9HMGtruncation_PGKpuroR
Plasmid#203964PurposeDox inducible myc tag truncated SOX9 without DNA-binding domain (noHMG)DepositorInsertSOX9-noHMG (Sox9 Mouse)
UseLentiviralTagsmycExpressionMutationthe HMG domain is truncatedPromoterTRE3GAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
3xMS-TR-100-PURO HRD
Plasmid#207582Purposehomologous recombination donor for the insertion of the 3xMS2-tag at the endogenous locusDepositorInsert3xMS2-TR and 100 bp of downstream sequence followed by a PuroR and flanked by endogenous TR sequences
UseTags3xMS2ExpressionMammalianMutationPromoterSV40Available sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
mCherry-Dyskerin
Plasmid#207577PurposeN-terminal mCherry-tag using SalI/EcoRIDepositorInsertgenerated by restriction cloning of gene into a modified pBABE-puro vector containing the N-terminal mCherrytag using SalI/EcoRI
UseTagsmCherryExpressionMammalianMutationPromoterLTRAvailable sinceJan. 16, 2025AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(711-800)_L786A
Plasmid#228838PurposeInsect cell expression of the mutant C-terminus of BICD2DepositorInsertBICD2 (Bicd2 Mouse)
UseTagsStrepExpressionInsectMutationaa 711-800, with L786APromoterAvailable sinceDec. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(711-800)
Plasmid#228833PurposeInsect cell expression of CC3 domain of BICD2 (aa 711-800)DepositorInsertBICD2 (Bicd2 Mouse)
UseTagsStrepExpressionInsectMutationaa 711-800PromoterAvailable sinceDec. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(342-800)
Plasmid#228832PurposeInsect cell expression of C-terminal portion of BICD2 (aa 342-800)DepositorInsertBICD2 (Bicd2 Mouse)
UseTagsStrepExpressionInsectMutationaa 342-800PromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pACEBac1_Strep-Psc-BICD2(711-800)_L742A
Plasmid#228839PurposeInsect cell expression of the mutant C-terminus of BICD2DepositorInsertBICD2 (Bicd2 Mouse)
UseTagsStrepExpressionInsectMutationaa 711-800, with L742APromoterAvailable sinceDec. 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh13CDS
Plasmid#192853PurposePlasmid to produce ISH probe for mMyh13 gene with CDS sequenceDepositorInsertmyosin heavy chain 13 (Myh13 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pGEMT-mMyh13UTR
Plasmid#192854PurposePlasmid to produce ISH probe for mMyh13 gene with UTR sequenceDepositorInsertmyosin heavy chain 13 (Myh13 Mouse)
UseTagsExpressionMutationPromoterAvailable sinceDec. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM59
Plasmid#227655PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, Δ78–81)DepositorUseTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, Δ78–81PromoterT7Available sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM57
Plasmid#227654PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, V80A)DepositorUseTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, V80APromoterT7Available sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAM54
Plasmid#227653PurposepETDuet-1: Rpn8(1-179)-PreScission-Strep, H6-PreScission-Rpn11(2-239, L132A)DepositorUseTagsH6-PreScission and PreScission-StrepExpressionBacterialMutation1-179 and amino acids 2-239, L132APromoterT7Available sinceDec. 4, 2024AvailabilityAcademic Institutions and Nonprofits only